Results 121 to 130 of about 1,291 (163)

A disector-based framework for the automatic optical fractionator

Journal of Chemical Neuroanatomy, 2022
Stereology-based methods provide the current state-of-the-art approaches for accurate quantification of numbers and other morphometric parameters of biological objects in stained tissue sections. The advent of artificial intelligence (AI)-based deep learning (DL) offers the possibility of improving throughput by automating the collection of stereology ...
Palak Dave, Dmitry B Goldgof, Lawrence O Hall   +2 more
exaly   +3 more sources

Practical considerations for the use of the optical disector in estimating neuronal number

Journal of Neuroscience Methods, 1994
The aim of this study was to establish methodological variability in the estimation of the total number of neurones using the optical disector. Variations in the 3 dimensions of the disector probe were analysed under uniform sampling conditions in 50-microns-thick frozen sections of the human mediodorsal thalamic nucleus.
A J Harding, Glenda M Halliday
exaly   +3 more sources

Software for counting cells and estimating structural volumes with the optical disector and fractionator

Microscopy Research and Technique, 1993
AbstractWe describe MS‐DOS software for the optical volume fractionator (OVF), a stereological method combining the principles of the optical disector (Gundersen et al.: Acta Pathol. Microbiol. Immunol. Scand., 96:857–881, 1988) and fractionator (Gundersen: J. Microsc., 143:3–45, 1986).
R P Bolender, Jay S Charleston
exaly   +3 more sources

Differential tissue shrinkage and compression in the z-axis: implications for optical disector counting in vibratome-, plastic- and cryosections

Journal of Neuroscience Methods, 2003
The optical disector is among the most efficient cell counting methods, but its accuracy depends on an undistorted particle distribution in the z-axis of tissue sections. Because the optical disector samples particle densities exclusively in the center of sections, it is essential for unbiased estimates of particle numbers that differential shrinkage ...
William J Hatton, Glenn D Rosen, Christopher S Von Bartheld   +2 more
exaly   +3 more sources

A brief update on physical and optical disector applications and sectioning-staining methods in neuroscience

Journal of Chemical Neuroanatomy, 2018
A quantitative description of a three-dimensional (3D) object based on two-dimensional images can be made using stereological methods These methods involve unbiased approaches and provide reliable results with quantitative data. The quantitative morphology of the nervous system has been thoroughly researched in this context.
Kıymet Kübra Yurt, Elfide Gizem Kivrak, Gamze Altun, Hamza Mohamed, Fathelrahman Ali, Hosam Eldeen Gasmalla, Suleyman Kaplan   +6 more
openaire   +3 more sources

Use of frozen sections to determine neuronal number in the murine hippocampus and neocortex using the optical disector and optical fractionator

Brain Research Protocols, 2004
Stereology is an important technique for the quantification of neurons in subregions of the central nervous system. A commonly used method of stereology relies upon embedment of tissue in glycol methacrylates to allow production of sections that are resistant to shrinkage in thickness. However, the use of glycol methacrylates for stereology has several
Daniel J Bonthius, Bahri Karacay, Nicholas J Pantazis   +2 more
exaly   +3 more sources

An Improved Method for the Production of Slides from Oversized Samples of Glycol Methacrylate-Embedded Tissues: Application for Optical Disector Based Stereology

Journal of Histotechnology, 2003
AbstractAn improvement in embedment and sectioning methodology of large tissue samples in glycol methacrylate resin is presented. The method improves efficiency by allowing relatively large tissue slabs, or multiple sets of smaller tissue samples to be embedded in large 2 × 3 inch blocks.
Angela C Thyer, Michael R Soules, Jay S Charleston   +2 more
exaly   +2 more sources

Physical and optical disectors

In morphometric studies, two-dimensional histological sections are evaluated using designbased stereological methods in order to obtain three-dimensional structural information about the tissue. The stereology is a combination of geometric measurements and statistical sampling principles.
Altun, Gamze, Elamin, Abdalla Ahmed Eldaw, Kaplan, Arife Ahsen, Kipanyula, Maulilio J., Kaya, Sefa Ersan, Kaplan, Süleyman   +5 more
openaire   +2 more sources

Loss of primary sensory neurons in the very old rat: Neuron number estimates using the disector method and confocal optical sectioning

Journal of Comparative Neurology, 1998
Loss of neurons has been considered to be a prime cause of nervous disturbances that occur with advancing age. However, the notion of a constitutive aging-related loss of neurons has been challenged recently in several studies that used up-to-date methods for counting neurons.
Esbjörn Bergman, Brun Ulfhake
exaly   +3 more sources

Analysis of cell death in the trochlear nucleus of the chick embryo: Calibration of the optical disector counting method reveals systematic bias

Journal of Comparative Neurology, 1999
Detection of changes in numbers of neurons is essential for an understanding of neuronal development, function, and death. Optical disector counting is claimed to be the most efficient technique to estimate accurate numbers of neurons in microscopic sections.
William J Hatton, Christopher S Von Bartheld   +1 more
exaly   +3 more sources