Results 71 to 80 of about 617,702 (267)
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
In this paper, the Pby (Zr1-xTix) O3 piezoelectric compounds prepared by the hydrothermal- autoclave and the effect of calcination in thermal furnace and microwave furnace on optical and dielectric properties has been studied.
Mohammad Hossein Doust +1 more
doaj +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
This work presents a systematic first-principles study of the crystal structure, mechanical, electrical, optical, and thermodynamic properties of lithium- and calcium-based catalysts (Li _3 N, Ca _3 N _2 , Li _3 BN _2 , and Ca _3 B _2 N _4 ) for the ...
Meizhe Lv, Xiaofei Guo, Lichao Cai
doaj +1 more source
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source
The objective of this research is to provide a detailed examination of the ways in which perovskite Ca3PCl3 can be improved for its efficiency in optoelectronic and solar cell field.
Chakshu Malan +2 more
doaj +1 more source
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley +1 more source
Marco Lombardo,1,2 Marianna Rosati,1 Marco Pileri,3 Domenico Schiano-Lomoriello,1 Sebastiano Serrao11Fondazione G.B. Bietti IRCCS, 2Vision Engineering Italy Srl, 3Azienda Ospedaliera San Giovanni-Addolorata, Rome, ItalyBackground: The purpose of this ...
Lombardo M +4 more
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