Results 71 to 80 of about 938,861 (316)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Optical characterization of CuIn5S8 crystals by ellipsometry measurements

, 2016
Optical properties of CuIn5S8 crystals grown by Bridgman method were investigated by ellipsometry measurements. Spectral dependence of optical parameters; real and imaginary parts of the pseudodielectric function, pseudorefractive index, pseudoextinction
IŞIK, MEHMET, Gasanly, Nizami
core   +1 more source

Applicability of mitotic figure counting by deep learning: a development and pan‐cancer validation study

FEBS Open Bio, EarlyView.
In this study, we developed a deep learning method for mitotic figure counting in H&E‐stained whole‐slide images and evaluated its prognostic impact in 13 external validation cohorts from seven different cancer types. Patients with more mitotic figures per mm2 had significantly worse patient outcome in all the studied cancer types except colorectal ...
Joakim Kalsnes, Maria X. Isaksen, Frida Julbø, Manohar Pradhan, Andreas Kleppe, Sepp De Raedt, Ole‐Johan Skrede, Turid Torheim, John Arne Nesheim, Hans Martin Mohn, Hanne A. Askautrud, Karolina Cyll, Wanja Kildal, Emma Rewcastle, Melinda Lillesand, Vebjørn Kvikstad, Emiel Janssen, Robert Jones, Odd Terje Brustugun, Bjørn Brennhovd, Erik Skaaheim Haug, Lill‐Tove Rasmussen Busund, Elin Richardsen, Sigve Andersen, Tom Dønnem, Kristina Lindemann, Gunnar Kristensen, Neil A. Shepherd, Marco Novelli, Knut Liestøl, David Kerr, Håvard E. Danielsen, Tarjei S. Hveem   +32 more
wiley   +1 more source

Nonlinear properties of silicon core optical fibres

, 2013
Silica optical fibres are renowned for the framework they have set in modern communications systems, sensors, and biotechnology. One particular trend in current research aims to investigate materials with enhanced optical functionality, high optical ...
Mehta, Priyanth
core  

Effect of Variation Consentration Ion Nd3+ on the Physical and Optical Properties of TZBN Glasses as a Host Material Glass Laser [PDF]

, 2012
This study aims to determine the effect of Nd ionconcentration on the physical and optical properties of tellurite glass. Tellurite glass used is TZBN (Tellurium Zinc Bismuth Natrium Oxide), with composition (mol%) 60TeO2-(35-x) ZnO-2Bi2O3-3Na2O, and
, Riyatun, Marzuki, Ahmad, Rahmasari, Lita, Nurmalasari, Ika, Riyatun,   +4 more
core  

Deriving inherent optical properties and associated inversion-uncertainties in the Dutch Lakes [PDF]

, 2009
Remote sensing of water quality in inland waters requires reliable retrieval algorithms, accurate atmospheric correction and consistent method for uncertainty estimation.
Dekker, A., C. M. Mannaerts, Salama, M.S., Z. Su, Salama, M.S.; id_orcid, Mannaerts, C.M., A. Dekker, W. Verhoef, Su, Z., M. S. Salama, Verhoef, W., Su, Z.; id_orcid, Dekker, A.G., Mannaerts, C.M.M.   +13 more
core   +1 more source

Importance of the layered structure of leaves on the determination of their scattering and absorption properties

Science of Remote Sensing
The influence of the layered structure of plant leaves on the optical properties obtained with a homogeneous theoretical model was investigated. To this aim, silicone phantoms with different optical properties modeling synthetic leaf layers were ...
Corinna Konrad, Markus Wagner, Florian Foschum, Alwin Kienle   +3 more
doaj   +1 more source

Sensitivity and Contribution of Organic Aerosols to Aerosol Optical Properties Based on Their Refractive Index and Hygroscopicity

Atmosphere, 2016
Organic carbon (OC) accounts for a large fraction of particulate matter. Since many atmospheric organic compounds have different optical properties, it is difficult to determine the optical properties of OC accurately.
Chang Hoon Jung, Hye Jung Shin, Ji Yi Lee, Yong Pyo Kim   +3 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source