We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Control Theory for a Class of 2D Continuous-Discrete Linear Systems
This paper considers a general class of 2D continuous-discrete linear systems of both systems theoretic and applications interest. The focus is on the development of a comprehensive control systems theory for members of this class in a unified manner ...
Owens, D H +4 more
core +1 more source
Application of Optimal Control Theory to Fourier Transform Ion Cyclotron Resonance. [PDF]
Martikyan V +4 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
The Reproductive Value in Distributed Optimal Control Models [PDF]
We show that in a large class of distributed optimal control models (DOCM), where population is described by a McKendrick type equation with an endogenous number of newborns, the reproductive value of Fisher shows up as part of the shadow price of the ...
Michael Kuhn +3 more
core
Mathematical assessment of constant and time-dependent control measures on the dynamics of the novel coronavirus: An application of optimal control theory. [PDF]
Zhang L +4 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Theory of Stochastic Optimal Economic Growth [PDF]
This paper is a survey of the theory of stochastic optimal economic growth.International Development,
Olson, Lars J., Roy, Santanu
core
Online-Computation Approach to Optimal Control of Noise-Affected Nonlinear Systems with Continuous State and Control Spaces [PDF]
22.10.13 KB. Ok to add author version to spiral.A novel online-computation approach to optimal control of nonlinear, noise-affected systems with continuous state and control spaces is presented.
Deisenroth, Marc P +3 more
core
CONTROL "IN THE SMALL" - A NEW DIRECTION IN THE MATHEMATICAL THEORY OF OPTIMAL CONTROL
Background. This article is devoted to the historiography of a new direction in mathematical control theory - control "in the small," discovered and currently being developed by scientists at the Ryazan State Radio Engineering University named after V.F.
Valentin V. Mironov
doaj +1 more source

