Results 51 to 60 of about 62,519 (299)

PLGA Nanofiber/PDMS Microporous Composite Membrane-Sandwiched Microchip for Drug Testing

Micromachines, 2020
Lung-on-a-chip devices could provide new strategies for a biomimetic lung cell microenvironment and construction of lung disease models in vitro, and are expected to greatly promote the development of drug evaluation, toxicological detection, and disease
Wei Li, Xindi Sun, Bing Ji, Xingyuan Yang, Bingpu Zhou, Zhanjun Lu, Xinghua Gao   +6 more
doaj   +1 more source

Alveolus Lung-on-a-Chip Platform: A Proposal

Chemosensors, 2021
Respiratory diseases are top-ranked causes of deaths and disabilities around the world, making new approaches to the treatment necessary. In recent years, lung-on-a-chip platforms have emerged as a potential candidate to replace animal experiments ...
Noelia Campillo, Vinicius Rosa Oliveira, Renata Kelly da Palma   +2 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Living Cell Microarrays: An Overview of Concepts [PDF]

, 2016
Living cell microarrays are a highly efficient cellular screening system. Due to the low number of cells required per spot, cell microarrays enable the use of primary and stem cells and provide resolution close to the single-cell level.
Jonczyk, Rebecca, Kurth, Tracy, Lavrentieva, Antonina, Scheper, Thomas, Stahl, Frank, Walter, Johanna-Gabriela   +5 more
core   +3 more sources

Somatic mutational landscape in von Hippel–Lindau familial hemangioblastoma

Molecular Oncology, EarlyView.
The causes of central nervous system (CNS) hemangioblastoma in Von Hippel–Lindau (vHL) disease are unclear. We used Whole Exome Sequencing (WES) on familial hemangioblastoma to investigate events that underlie tumor development. Our findings suggest that VHL loss creates a permissive environment for tumor formation, while additional alterations ...
Maja Dembic, Anne Nørremølle, Lilian Bomme Ousager, Lars van Brakel Andersen, Marie Louise Mølgaard Binderup, Mads Thomassen   +5 more
wiley   +1 more source

Current approaches and future role of high content imaging in safety sciences and drug discovery [PDF]

, 2014
High content imaging combines automated microscopy with image analysis approaches to simultaneously quantify multiple phenotypic and/or functional parameters in biological systems.
Beilmann M., DANESIAN Mardas, DAVIES Anthony, FAVA Eugenio, FLECK Roland, Hartung T., JULÉ Yvon, KANSY Manfred, KUSTERMANN Stefan, LEIST Marcel, MACKO Peter, MUNDY William, ROTH Adrian, SHAH Imran, UTENG Marianne, VAN DE WATER Bob, VAN VLIET Erwin   +16 more
core   +1 more source

Organ on a chip

Colloids and Surfaces B: Biointerfaces
Organ-on-a-chip systems, also referred to as microphysiological systems (MPS), represent an advance in bioengineering microsystems designed to mimic key aspects of human organ physiology and function. Drawing inspiration from the intricate and hierarchical architecture of the human body, these innovative platforms have emerged as invaluable in vitro ...
Aarif Ali, Ishteyaq Majeed Shah, Mohammad Saleem Bhat, Zainab Manzoor, Aadil Ayaz, Muneeb U. Rehman, Sheikh Bilal Ahmad   +6 more
openaire   +2 more sources

Organ-On-A-Chip: An Emerging Research Platform

Organogenesis, 2023
In drug development, conventional preclinical and clinical testing stages rely on cell cultures and animal experiments, but these methods may fall short of fully representing human biology. To overcome this limitation, the emergence of organ-on-a-chip (OOC) technology has sparked interest as a transformative approach in drug testing research.
Nithin R, Ayushi Aggarwal, Anne Boyina Sravani, Pooja Mallya, Shaila Lewis   +4 more
openaire   +3 more sources

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source