Nucleosomes influence multiple steps during replication initiation [PDF]
eLife, 2017 Eukaryotic replication origin licensing, activation and timing are influenced by chromatin but a mechanistic understanding is lacking. Using reconstituted nucleosomal DNA replication assays, we assessed the impact of nucleosomes on replication initiation.
Ishara F Azmi +7 more
doaj +5 more sources
Identification of the Unwinding Region in the Clostridioides difficile Chromosomal Origin of Replication [PDF]
Frontiers in Microbiology, 2020 Faithful DNA replication is crucial for viability of cells across all kingdoms. Targeting DNA replication is a viable strategy for inhibition of bacterial pathogens.
Ana M. Oliveira Paiva +6 more
doaj +2 more sources
E2 protein is the major determinant of specificity at the human papillomavirus origin of replication. [PDF]
PLoS ONE, 2019 The replication of human papillomavirus (HPV) genomes requires E1 and E2 proteins as the viral trans-factors and the replication origin, located in the URR, as a cis-element.
Airiin Laaneväli +3 more
doaj +2 more sources
LSD1 is required for euchromatic origin firing and replication timing [PDF]
Signal Transduction and Targeted Therapy, 2022 The chromatin-based rule governing the selection and activation of replication origins remains to be elucidated. It is believed that DNA replication initiates from open chromatin domains; thus, replication origins reside in open and active chromatin ...
Yue Wang +9 more
doaj +2 more sources
Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe. [PDF]
Genome Biol, 2015 Background DNA replication initiates on defined genome sites, termed origins. Origin usage appears to follow common rules in the eukaryotic organisms examined to date: all chromosomes are replicated from multiple origins, which display variations in ...
Marques CA +4 more
europepmc +4 more sources
Genome Replication in Thermococcus kodakarensis Independent of Cdc6 and an Origin of Replication [PDF]
Frontiers in Microbiology, 2017 The initiation of DNA replication is typically tightly regulated by proteins that form initiation complexes at specific sequences known as replication origins.
Alexandra M. Gehring +7 more
doaj +2 more sources
On the Choice of the Right Plasmid Vector(s) in the Times of Synthetic Biology [PDF]
Microbial BiotechnologyPlasmid vectors are to this day the fundamental tools in molecular biology, but their selection is often guided by convenience rather than informed choice. This article revisits the architectural and functional features that determine plasmid performance
Víctor deLorenzo +1 more
doaj +2 more sources
The Replicative Consequences of Papillomavirus E2 Protein Binding to the Origin Replication Factor ORC2. [PDF]
PLoS Pathogens, 2016 The origin recognition complex (ORC) coordinates a series of events that lead to initiation of DNA strand duplication. As a nuclear double stranded DNA plasmid, the papillomavirus (PV) genome resembles a mini-chromosome in infected cells. To initiate its
Marsha DeSmet +5 more
doaj +5 more sources
Role for RNA:DNA hybrids in origin-independent replication priming in a eukaryotic system. [PDF]
Proc Natl Acad Sci U S A, 2015 DNA replication initiates at defined replication origins along eukaryotic chromosomes, ensuring complete genome duplication within a single S-phase. A key feature of replication origins is their ability to control the onset of DNA synthesis mediated by ...
Stuckey R +3 more
europepmc +3 more sources
Functional characterization of the origin of replication of pRN1 from Sulfolobus islandicus REN1H1. [PDF]
PLoS ONE, 2013 Plasmid pRN1 from Sulfolobus islandicus REN1H1 is believed to replicate by a rolling circle mechanism but its origin and mechanism of replication are not well understood.
Chijioke J Joshua +2 more
doaj +2 more sources