Results 141 to 150 of about 1,409,521 (314)

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Chromatin’s Influence on Pre-Replication Complex Assembly and Function

Biology
In all eukaryotes, the initiation of DNA replication requires a stepwise assembly of factors onto the origins of DNA replication. This is pioneered by the Origin Recognition Complex, which recruits Cdc6.
Hina Ahmad, Neha Chetlangia, Supriya G. Prasanth   +2 more
doaj   +1 more source

Control of DNA Rereplication via Cdc2 Phosphorylation Sites in the Origin Recognition Complex [PDF]

, 2001
A Vas, Winnie Mok, Janet Leatherwood
openalex   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Diagnostic value of serum origin recognition complex protein 1 in the progression of HBVrelated liver fibrosis

Guangxi Yike Daxue xuebao
Objective To explore the diagnostic value and significance of human serum origin recognition complex protein 1 (ORC1) in the degree of hepatitis B (HBV)-related liver fibrosis. Methods A total of 269 patients with HBV infection admitted to the Department
LIANG Hongqian, HUANG Long, REN Aoli, SU Minghua, HU Bobin, LI Qingmei, SU Tumei, YIN Qianbing, LI Aining, JIANG Jianning   +9 more
doaj   +1 more source

EBNA1-mediated recruitment of a histone H2B deubiquitylating complex to the Epstein-Barr virus latent origin of DNA replication.

PLoS Pathogens, 2009
The EBNA1 protein of Epstein-Barr virus (EBV) plays essential roles in enabling the replication and persistence of EBV genomes in latently infected cells and activating EBV latent gene expression, in all cases by binding to specific recognition sites in ...
Feroz Sarkari, Teresa Sanchez-Alcaraz, Shan Wang, Melissa N Holowaty, Yi Sheng, Lori Frappier   +5 more
doaj   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

BAHCC1 binds H4K20me1 to facilitate the MCM complex loading and DNA replication

Nature Communications
Mono-methylation of histone H4 lysine 20 (H4K20me1) regulates DNA replication, cell cycle progression and DNA damage repair. How exactly H4K20me1 regulates these biological processes remains unclear.
Dongxu Li, Zhi-Min Zhang, Liu Mei, Yao Yu, Yiran Guo, Samuel G. Mackintosh, Jianbin Chen, David F. Allison, Arum Kim, Aaron J. Storey, Ricky D. Edmondson, Stephanie D. Byrum, Alan J. Tackett, Ling Cai, Jeanette G. Cook, Jikui Song, Gang Greg Wang   +16 more
doaj   +1 more source

latheo Encodes a Subunit of the Origin Recognition Complex and Disrupts Neuronal Proliferation and Adult Olfactory Memory When Mutant [PDF]

, 1999
Shirly Pinto, David G. Quintana, Patrick Smith, Robert Mihalek, Zhi-hui Hou, S Boynton, Christopher J. Jones, Marvin Hendricks, Klara Velinzon, James A. Wohlschlegel, Richard J. Austin, William S. Lane, Tim Tully, Anindya Dutta   +13 more
openalex   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source