Multitarget Mechanisms of (‒)‒Epigallocatechin-3-Gallate Against MRSA: From SraP L-Lectin Targeting to Synergistic Antibiotic Effects. [PDF]
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A catalog of proteins released from <i>Asaia bogorensis</i> under two growth conditions. [PDF]
Manges AB, Lampe DJ.
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Recent Advances in Heterologous Protein Expression and Natural Product Synthesis by <i>Aspergillus</i>. [PDF]
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Transformation of Aspergillus niger using the homologous orotidine-5′-phosphate-decarboxylase gene
Current Genetics, 1987A homologous transformation system for the filamentous fungus Aspergillus niger has been developed, based on the orotidine-5'-phosphate-decarboxylase gene. A. niger Pyr- mutants have been selected from 5-fluoro-orotic acid resistant mutants. These mutants were found to comprise two complementation groups, pyrA and pyrB. The A.
Goosen, T. +5 more
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Studies of orotidine 5′-phosphate decarboxylase in Neurospora crassa
Archives of Biochemistry and Biophysics, 1967Abstract Extracts prepared from wild-type Neurospora crassa, strain 1A, contain an active orotidylate decarboxylase with properties generally similar to those reported for the comparable yeast enzyme (orotidine 5′-phosphate carboxy-lyase, E.C.4.1.1.23). A pyrimidine-requiring mutant, N.
Thomas I. Pynadath, Robert M. Fink
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Contribution of Enzyme−Phosphoribosyl Contacts to Catalysis by Orotidine 5‘-Phosphate Decarboxylase
Biochemistry, 2000The crystal structure of the complex formed between recombinant yeast orotidine 5'-phosphate decarboxylase and the competitive inhibitor 6-hydroxyuridine 5'-phosphate reveals the presence of four hydrogen bonds between active site residues Tyr-217 and Arg-235 and the phosphoryl group of this inhibitor.
B G, Miller +3 more
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An Alternative Explanation for the Catalytic Proficiency of Orotidine 5‘-Phosphate Decarboxylase
Journal of the American Chemical Society, 2001Orotidine 5'-phosphate decarboxylase (ODCase) is the most proficient enzyme known, enhancing the rate of decarboxylation of orotidine 5'-phosphate (OMP) by a factor of 10(17), which corresponds to a DeltaDeltaG++ of approximately 24 kcal/mol. Ground-state destabilization through local electrostatic stress has been recently proposed as the basis of ...
T S, Lee +3 more
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Isolation and characterization of Candida kefyr orotidine‐5′‐phosphate decarboxylase (URA3) gene
Yeast, 2009AbstractCandida kefyr is a common yeast species that can be found in fermented milk and cheeses. As a first step to developing a gene transfer system for C. kefyr, the orotidine‐5′‐phosphate decarboxylase (URA3) gene was cloned, using degenerate PCR and genome walking. The uninterrupted open reading frame of the C.
Chu, JKM, Tsang, PWK, Wong, KS
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Cloning of the PYR4 gene encoding orotidine-5?-phosphate decarboxylase in Cephalosporium acremonium
Current Genetics, 1990We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5'-phosphate decarboxylase (OMPdecase), and most probably does not contain introns ...
A, Vian, M A, Peñalva
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