Results 121 to 130 of about 4,091 (157)

Transformation of Aspergillus niger using the homologous orotidine-5′-phosphate-decarboxylase gene

Current Genetics, 1987
A homologous transformation system for the filamentous fungus Aspergillus niger has been developed, based on the orotidine-5'-phosphate-decarboxylase gene. A. niger Pyr- mutants have been selected from 5-fluoro-orotic acid resistant mutants. These mutants were found to comprise two complementation groups, pyrA and pyrB. The A.
Goosen, T.   +5 more
openaire   +3 more sources

Studies of orotidine 5′-phosphate decarboxylase in Neurospora crassa

Archives of Biochemistry and Biophysics, 1967
Abstract Extracts prepared from wild-type Neurospora crassa, strain 1A, contain an active orotidylate decarboxylase with properties generally similar to those reported for the comparable yeast enzyme (orotidine 5′-phosphate carboxy-lyase, E.C.4.1.1.23). A pyrimidine-requiring mutant, N.
Thomas I. Pynadath, Robert M. Fink
openaire   +1 more source

Contribution of Enzyme−Phosphoribosyl Contacts to Catalysis by Orotidine 5‘-Phosphate Decarboxylase

Biochemistry, 2000
The crystal structure of the complex formed between recombinant yeast orotidine 5'-phosphate decarboxylase and the competitive inhibitor 6-hydroxyuridine 5'-phosphate reveals the presence of four hydrogen bonds between active site residues Tyr-217 and Arg-235 and the phosphoryl group of this inhibitor.
B G, Miller   +3 more
openaire   +2 more sources

An Alternative Explanation for the Catalytic Proficiency of Orotidine 5‘-Phosphate Decarboxylase

Journal of the American Chemical Society, 2001
Orotidine 5'-phosphate decarboxylase (ODCase) is the most proficient enzyme known, enhancing the rate of decarboxylation of orotidine 5'-phosphate (OMP) by a factor of 10(17), which corresponds to a DeltaDeltaG++ of approximately 24 kcal/mol. Ground-state destabilization through local electrostatic stress has been recently proposed as the basis of ...
T S, Lee   +3 more
openaire   +2 more sources

Isolation and characterization of Candida kefyr orotidine‐5′‐phosphate decarboxylase (URA3) gene

Yeast, 2009
AbstractCandida kefyr is a common yeast species that can be found in fermented milk and cheeses. As a first step to developing a gene transfer system for C. kefyr, the orotidine‐5′‐phosphate decarboxylase (URA3) gene was cloned, using degenerate PCR and genome walking. The uninterrupted open reading frame of the C.
Chu, JKM, Tsang, PWK, Wong, KS
openaire   +4 more sources

Cloning of the PYR4 gene encoding orotidine-5?-phosphate decarboxylase in Cephalosporium acremonium

Current Genetics, 1990
We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5'-phosphate decarboxylase (OMPdecase), and most probably does not contain introns ...
A, Vian, M A, Peñalva
openaire   +2 more sources

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