Results 131 to 140 of about 4,091 (157)
Some of the next articles are maybe not open access.
Transformation of Endomyces fibuliger based on its gene for orotidine-5′-phosphate decarboxylase
Gene, 2000Endomyces fibuliger is a yeast used in the production of Chinese rice wine. It secretes enzymes such as glucoamylase, alpha-amylase and acid protease. Very little is known of the genetics of E. fibuliger. In order to develop a transformation system for this yeast, orotidine-5'-phosphate decarboxylase mutant strains were obtained and characterized ...
Ngan, W.Y. +4 more
openaire +2 more sources
Biochemie und Physiologie der Pflanzen, 1981
Summary Orotate phosphoribosyltransferase (OPRTase, EC 2.4.2.10 and orotidine-5' -phosphate decarboxylase (ODCase, EC 4.1.1.23) were purified from Euglena gracilis about 120-fold and characterized. Enzyme activities were not separable by ammonium sulphate fractionation and chromatography using Sephadex G-150 and DEAE-Sephadex A-25 suggesting that ...
R. Walther, G.-J. Krauss, H. Reinbothe
openaire +1 more source
Summary Orotate phosphoribosyltransferase (OPRTase, EC 2.4.2.10 and orotidine-5' -phosphate decarboxylase (ODCase, EC 4.1.1.23) were purified from Euglena gracilis about 120-fold and characterized. Enzyme activities were not separable by ammonium sulphate fractionation and chromatography using Sephadex G-150 and DEAE-Sephadex A-25 suggesting that ...
R. Walther, G.-J. Krauss, H. Reinbothe
openaire +1 more source
Biochemical and Biophysical Research Communications, 1983
Relief of an auxotrophic requirement for uridine in Aspergillus nidulans strain G191 has been achieved by transformation with a segment of Neurospora crassa DNA containing the corresponding gene coding for orotidine-5'-phosphate decarboxylase. The mitotic stability of such transformants suggests that the DNA has integrated into the genome.
D J, Ballance, F P, Buxton, G, Turner
openaire +2 more sources
Relief of an auxotrophic requirement for uridine in Aspergillus nidulans strain G191 has been achieved by transformation with a segment of Neurospora crassa DNA containing the corresponding gene coding for orotidine-5'-phosphate decarboxylase. The mitotic stability of such transformants suggests that the DNA has integrated into the genome.
D J, Ballance, F P, Buxton, G, Turner
openaire +2 more sources
Current Genetics, 1980
A mutant yeast constitutive for synthesis of both dihydroorotic acid dehydrogenase and orotidine 5' phosphate decarboxylase has been isolated. This phenotype is due to a single gene unlinked to any of the five genes of the pyrimidine pathway. This gene, called ppr1, induces the unlinked genes ural and ura3. Non-chromosomal cloned ura3 is also under the
G, Loison, R, Losson, F, Lacroute
openaire +2 more sources
A mutant yeast constitutive for synthesis of both dihydroorotic acid dehydrogenase and orotidine 5' phosphate decarboxylase has been isolated. This phenotype is due to a single gene unlinked to any of the five genes of the pyrimidine pathway. This gene, called ppr1, induces the unlinked genes ural and ura3. Non-chromosomal cloned ura3 is also under the
G, Loison, R, Losson, F, Lacroute
openaire +2 more sources
Molecular and General Genetics MGG, 1990
The pyrG gene of Phycomyces was isolated from a Phycomyces genomic library, constructed in the cosmid pHS255, by hybridization with a 170 bp fragment of the pyrG gene of Aspergillus niger. This fragment includes a consensus sequence found in almost all species in which the orotidine-5'-phosphate decarboxylase (OMPdecase) gene has been sequenced.
J M, Díaz-Mínguez +4 more
openaire +2 more sources
The pyrG gene of Phycomyces was isolated from a Phycomyces genomic library, constructed in the cosmid pHS255, by hybridization with a 170 bp fragment of the pyrG gene of Aspergillus niger. This fragment includes a consensus sequence found in almost all species in which the orotidine-5'-phosphate decarboxylase (OMPdecase) gene has been sequenced.
J M, Díaz-Mínguez +4 more
openaire +2 more sources
Current Genetics, 1989
The human pathogen Candida albicans grows either as a yeast or in filamentous form. We have determined the sequence of a 1.365 kb genomic C. albicans fragment that complements Saccharomyces cerevisiae ura3 and Escherichia coli pyrF mutations. An open reading frame within this fragment corresponds to a protein of 270 amino acids that shows homology to ...
C, Losberger, J F, Ernst
openaire +2 more sources
The human pathogen Candida albicans grows either as a yeast or in filamentous form. We have determined the sequence of a 1.365 kb genomic C. albicans fragment that complements Saccharomyces cerevisiae ura3 and Escherichia coli pyrF mutations. An open reading frame within this fragment corresponds to a protein of 270 amino acids that shows homology to ...
C, Losberger, J F, Ernst
openaire +2 more sources
Current Microbiology, 1998
An integrative transformation system was established for a phenol-utilizing strain of Candida tropicalis M4. The system is based on an auxotrophic mutant host of C. tropicalis U-6 that is defective in orotidine-5'-phosphate decarboxylase (ODCase). As a selectable marker, we isolated and characterized the C. tropicalis URA3 gene, which codes for ODCase.
J H, Su, J H, Hsia, M C, Chang
openaire +2 more sources
An integrative transformation system was established for a phenol-utilizing strain of Candida tropicalis M4. The system is based on an auxotrophic mutant host of C. tropicalis U-6 that is defective in orotidine-5'-phosphate decarboxylase (ODCase). As a selectable marker, we isolated and characterized the C. tropicalis URA3 gene, which codes for ODCase.
J H, Su, J H, Hsia, M C, Chang
openaire +2 more sources
Journal of Fermentation and Bioengineering, 1992
Abstract The URA3 gene, encoding orotidine-5′-phosphate decarboxylase, was isolated from a Hansenula anomata genomic library by hybridization with the Saccharomyces cerevisiae URA3 gene. The nucleotide sequence and the transcriptional starting points were determined.
Tomoo Ogata +3 more
openaire +1 more source
Abstract The URA3 gene, encoding orotidine-5′-phosphate decarboxylase, was isolated from a Hansenula anomata genomic library by hybridization with the Saccharomyces cerevisiae URA3 gene. The nucleotide sequence and the transcriptional starting points were determined.
Tomoo Ogata +3 more
openaire +1 more source
Journal of Medicinal Chemistry, 1979
Condensation of 6-azauridine with ethyl levulinate, followed by saponification or phosphorylation, leads to 2',3'-O-[1-(2-carboxyethyl)ethylidene]-6-azauridine and its 5'-monophosphate. The latter was coupled to 6-aminohexylagarose via its carboxylic group. Using the same synthetic route, agarose-linked uridine 5'-monophosphate has been prepared.
H, Rosemeyer, F, Seela
openaire +2 more sources
Condensation of 6-azauridine with ethyl levulinate, followed by saponification or phosphorylation, leads to 2',3'-O-[1-(2-carboxyethyl)ethylidene]-6-azauridine and its 5'-monophosphate. The latter was coupled to 6-aminohexylagarose via its carboxylic group. Using the same synthetic route, agarose-linked uridine 5'-monophosphate has been prepared.
H, Rosemeyer, F, Seela
openaire +2 more sources
Gene, 1994
The pyr4 gene, encoding orotidine-5'-phosphate decarboxylase (OMP decarboxylase) from the biocontrol fungus Trichoderma harzianum, has been isolated by hybridization, using a polymerase chain reaction (PCR)-derived fragment as a probe. The PCR primers corresponded to conserved regions of OMP decarboxylase-encoding genes from other filamentous fungi.
E J, Heidenreich, C P, Kubicek
openaire +2 more sources
The pyr4 gene, encoding orotidine-5'-phosphate decarboxylase (OMP decarboxylase) from the biocontrol fungus Trichoderma harzianum, has been isolated by hybridization, using a polymerase chain reaction (PCR)-derived fragment as a probe. The PCR primers corresponded to conserved regions of OMP decarboxylase-encoding genes from other filamentous fungi.
E J, Heidenreich, C P, Kubicek
openaire +2 more sources