Results 31 to 40 of about 4,091 (157)

Self-establishing communities enable cooperative metabolite exchange in a eukaryote [PDF]

, 2015
Metabolite exchange among co-growing cells is frequent by nature, however, is not necessarily occurring at growth-relevant quantities indicative of non-cell-autonomous metabolic function.
Baker Brachmann, Bilsland, Blaser, Bonuccelli, Braakman, Buescher, Bélanger, Caetano-Anollés, Canelas, Castrillo, Christianson, Costerton, Dobay, Findley, Foster, Freilich, Ghosh, Harcombe, Hom, Horai, Jeffares, Johnson, Kahm, Liti, Manor, Mee, Momeni, Mumberg, Mülleder, Müller, Nigam, Oliveira, Paczia, Pande, Piedrafita, Pronk, Ramsey, Sheff, Shou, Sikorski, Silva, Sivakumar, Smukalla, Stahl, Teng, Vetsigian, Volkenhoff, Wintermute, Yazaki, Zelezniak   +49 more
core   +3 more sources

DNA demethylation pathways: Additional players and regulators. [PDF]

, 2017
DNA demethylation can occur passively by "dilution" of methylation marks by DNA replication, or actively and independently of DNA replication. Direct conversion of 5-methylcytosine (5mC) to cytosine (C), as originally proposed, does not occur.
Abdouni, An, Arakawa, Barreto, Bellon, Bhutani, Bhutani, Blaschke, Boorstein, Bourc'his, Bransteitter, Brown, Buganim, Chakraborty, Chen, Chen, Chen, Chowdhury, Chung, Cortazar, Cortellino, Costa, Dai, Dai, Dang, Dawlaty, Dawlaty, Dawlaty, Delhommeau, Doege, Dominguez, Engel, Fortini, Foshay, Fousteri, Franchini, Fritz, Fritz, Gao, Ge, Gehring, Gjini, Grin, Gu, Guo, Guo, Habib, Hajkova, Hashimoto, Hashimoto, Hashimoto, Hashimoto, Hata, Hata, He, Henson, Hildrestrand, Hogenbirk, Hollander, Hon, Hu, Hu, Iiams, Inoue, Iqbal, Ito, Iyer, Jin, Jin, Jiricny, Jones, Kagiwada, Kaneda, Kellinger, Ko, Kosmider, Kou, Kriaucionis, Krokan, Kumar, Langemeijer, Larijani, Laukka, Lenz, Li, Li, Li, Li, Liu, Liutkeviciute, Lorsbach, Lu, Ma, Maiti, Maiti, Marusawa, Mayer, Moran-Crusio, Muller, Muramatsu, Muramatsu, Nabel, Nakanishi, Nemec, Niehrs, Nilsen, Ohno, Okano, Ooi, Pais, Pastor, Perez, Petersen-Mahrt, Pfaffeneder, Pollard, Popp, Rai, Raiber, Ramiro, Rangam, Revy, Rommel, Rusmintratip, Sabag, Sajadian, Santos, Schiesser, Schiesser, Schmitz, Schomacher, Sciacovelli, Sejersted, Shen, Shen, Shibutani, Shimamoto, Shimoda, Silverman, Spruijt, Stier, Szulik, Tahiliani, Tamura, Tan, Vartanian, Verdine, Wagner, Wang, Weber, Wijesinghe, Wojciechowski, Wu, Xiao, Xu, Xu, Xu, Xue, Yamazaki, Yin, Young, Zanotti, Zhao, Zhu   +162 more
core   +1 more source

Purification and characterization of orotidine-5'-phosphate decarboxylase from Escherichia coli K-12. [PDF]

Journal of Bacteriology, 1983
Using blue Sepharose affinity chromatography, we purified orotidine-5'-phosphate decarboxylase over 600-fold, to near homogeneity, from strains of Escherichia coli harboring the cloned pyrF gene on the multicopy plasmid pDK26. The purified enzyme has a subunit molecular weight of 27,000 but appears to be catalytically active as a dimer.
W P, Donovan, S R, Kushner
openaire   +2 more sources

Construction of a flocculent saccharomyces cerevisiae fermenting lactose [PDF]

, 1999
A flocculent Saccharomyces cerevisiae strain with the ability to express both the LAC4 (coding for b-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus was constructed.
Domingues, Lucília, Lima, Nelson, Teixeira, J. A.   +2 more
core   +1 more source

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker. [PDF]

, 2011
Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon selection of single-crossover clones followed by counter-selection of double-crossover clones.
Cartman, ST, Cooksley, CM, Ehsaan, M, Heap, JT, Minton, NP, Ng, YK, Winzer, K   +6 more
core   +5 more sources

Quantitative Analysis of Candida Cell Wall Components by Flow Cytometrywith Triple-Fluorescence Staining [PDF]

, 2017
This work was supported by the European Commission within the FP7 Framework Programme [Fungitect-Grant No 602125]. We also thank Thomas Sauer, Vienna Biocenter Campus (VBC), Austria, for technical support at the FACS facility of the MFPL, Karl Kuchler ...
Gow, N, Istel, F, Jenull, S, Lion, T, Nogueira, MF, Walker, L A   +5 more
core   +1 more source

Orotic Aciduria [PDF]

, 2018
Orotic acid is an intermediate found in the pathway for pyrimidine synthesis. The mitochondrial enzyme dihydroorotate dehydrogenase (DHODH) catalyzes the production of orotic acid by the conversion of the compound dihydroorotate to orotic acid.
Fonteh, Aliah L
core   +1 more source

Purification and Kinetic Properties of Brain Orotidine 5′-Phosphate Decarboxylase

Journal of Biological Chemistry, 1968
Abstract Orotidine 5'-phosphate decarboxylase has been purified approximately 600-fold from cow brain. The purified enzyme catalyzes the irreversible decarboxylation of orotidine 5'-phosphate to form uridine 5'-phosphate and is inhibited by cytidine monophosphate g uridine monophosphate g cytidine diphosphate g uridine diphosphate g cytidine ...
openaire   +2 more sources

Centromeric Sequences in Ogataea polymorpha Genome Enable Development of Stable Multigene Expression Plasmid Tools

Microbial Biotechnology, Volume 18, Issue 11, November 2025.
The centromeric sequences of Ogataea polymorpha, a methylotrophic non‐conventional yeast, were characterised for the first time and support the development of new genetically stable centromeric plasmids with high genetic stability and compatibility, providing powerful genetic toolboxes for the synthetic biology and metabolic engineering‐driven ...
Yanfei Cheng, Yuanyuan Shi, Ning Sun, Xin Zhang, Mengwei Sun, Xiuping He   +5 more
wiley   +1 more source

The purification of orotidine-5'-phosphate decarboxylase from yeast by affinity chromatography.

Journal of Biological Chemistry, 1979
We have prepared an affinity column for the purification of orotidine-5'-phosphate decarboxylase from yeast. The column effects a 3200-fold purification from yeast homogenate in one pass; simple additional steps produce enzyme that has been purified 6700-fold and is not contaminated by any other protein that can be detected by sodium dodecyl sulfate ...
R S, Brody, F H, Westheimer
openaire   +2 more sources