Results 41 to 50 of about 4,196 (182)
Self-establishing communities enable cooperative metabolite exchange in a eukaryote [PDF]
Metabolite exchange among co-growing cells is frequent by nature, however, is not necessarily occurring at growth-relevant quantities indicative of non-cell-autonomous metabolic function.
Baker Brachmann +49 more
core +3 more sources
DNA demethylation pathways: Additional players and regulators. [PDF]
DNA demethylation can occur passively by "dilution" of methylation marks by DNA replication, or actively and independently of DNA replication. Direct conversion of 5-methylcytosine (5mC) to cytosine (C), as originally proposed, does not occur.
Abdouni +162 more
core +1 more source
Construction of a flocculent saccharomyces cerevisiae fermenting lactose [PDF]
A flocculent Saccharomyces cerevisiae strain with the ability to express both the LAC4 (coding for b-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus was constructed.
Domingues, Lucília +2 more
core +1 more source
Quantitative Analysis of Candida Cell Wall Components by Flow Cytometrywith Triple-Fluorescence Staining [PDF]
This work was supported by the European Commission within the FP7 Framework Programme [Fungitect-Grant No 602125]. We also thank Thomas Sauer, Vienna Biocenter Campus (VBC), Austria, for technical support at the FACS facility of the MFPL, Karl Kuchler ...
Gow, N +5 more
core +1 more source
Orotic acid is an intermediate found in the pathway for pyrimidine synthesis. The mitochondrial enzyme dihydroorotate dehydrogenase (DHODH) catalyzes the production of orotic acid by the conversion of the compound dihydroorotate to orotic acid.
Fonteh, Aliah L
core +1 more source
Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker. [PDF]
Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon selection of single-crossover clones followed by counter-selection of double-crossover clones.
Cartman, ST +6 more
core +5 more sources
The centromeric sequences of Ogataea polymorpha, a methylotrophic non‐conventional yeast, were characterised for the first time and support the development of new genetically stable centromeric plasmids with high genetic stability and compatibility, providing powerful genetic toolboxes for the synthetic biology and metabolic engineering‐driven ...
Yanfei Cheng +5 more
wiley +1 more source
Identification of MscS as a Key L‐Glutamate Exporter in Bacillus methanolicus
l‐Glutamate export in the methylotrophic bacterium Bacillus methanolicus, driven by an MscS‐like mechanosensitive channel. Figure created with BioRender (https://BioRender.com/t99s28). ABSTRACT Small‐conductance mechanosensitive channels (MscS) are established l‐glutamate exporters in industrially relevant bacteria, yet their role in the methylotrophic
Luciana Fernandes Brito +5 more
wiley +1 more source
Deletion of the Zinc Transporter Lipoprotein AdcAII Causes Hyperencapsulation of Streptococcus pneumoniae Associated with Distinct Alleles of the Type I Restriction-Modification System. [PDF]
The capsule is the dominant Streptococcus pneumoniae virulence factor, yet how variation in capsule thickness is regulated is poorly understood. Here, we describe an unexpected relationship between mutation of adcAII, which encodes a zinc uptake ...
Arioli +51 more
core +4 more sources
A successful transformation procedure for the genetic manipulation of the fungus Talaromyces amestolkiae was developed, enabling the forced expression of the transcriptional activator XlnR. A mutant version of XlnR carrying a Val785Phe substitution led to remarkably higher β‐xylosidase and endoxylanase activities compared to the wild‐type strain, which
Ana Pozo‐Rodríguez +4 more
wiley +1 more source

