Estimates of RelSeq, Mesh1, and SAHMex Hydrolysis of (p)ppGpp and (p)ppApp by Thin Layer Chromatography and NADP/NADH Coupled Assays [PDF]
Frontiers in Microbiology, 2020 The Mesh1 class of hydrolases found in bacteria, metazoans and humans was discovered as able to cleave an intact pyrophosphate residue esterified on the 3′hydroxyl of (p)ppGpp in a Mn2+ dependent reaction.
Katarzyna Potrykus +2 more
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Bacteria export alarmone synthetases that produce (p)ppApp and (p)ppGpp [PDF]
MBioGuanosine penta- and tetraphosphate [(p)ppGpp] and their adenosine analogs [(p)ppApp] are bacterial second messengers known as alarmones. Members of the RelA-SpoT homolog (RSH) family synthesize (p)ppGpp to mediate the stringent response during nutrient ...
Shehryar Ahmad +10 more
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Identification of a broadly conserved family of enzymes that hydrolyze (p)ppApp [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2023 Bacteria produce a variety of nucleotide second messengers to adapt to their surroundings. Although chemically similar, the nucleotides guanosine penta- and tetraphosphate [(p)ppGpp] and adenosine penta- and tetraphosphate [(p)ppApp] have distinct functions in bacteria. (p)ppGpp mediates survival under nutrient-limiting conditions and its intracellular
Shehryar Ahmad +2 more
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Structure-function comparisons of (p)ppApp vs (p)ppGpp for Escherichia coli RNA polymerase binding sites and for rrnB P1 promoter regulatory responses in vitro [PDF]
Biochimica Et Biophysica Acta - Gene Regulatory Mechanisms, 2018 Precise regulation of gene expression is crucial for bacteria to respond to changing environmental conditions. In addition to protein factors affecting RNA polymerase (RNAP) activity, second messengers play an important role in transcription regulation, such as well-known effectors of the stringent response: guanosine 5'triphosphate-3'diphosphate and ...
Bozena Bruhn-Olszewska +2 more
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An interbacterial toxin inhibits target cell growth by synthesizing (p)ppApp [PDF]
Nature, 2019 Bacteria have evolved sophisticated mechanisms to inhibit the growth of competitors1. One such mechanism involves type VI secretion systems, which bacteria can use to inject antibacterial toxins directly into neighbouring cells. Many of these toxins target the integrity of the cell envelope, but the full range of growth inhibitory mechanisms remains ...
Shehryar Ahmad +2 more
exaly +6 more sources
Small Alarmone Synthetase SasA Expression Leads to Concomitant Accumulation of pGpp, ppApp, and AppppA in Bacillus subtilis [PDF]
Frontiers in Microbiology, 2020 (p)ppGpp is a highly conserved bacterial alarmone which regulates many aspects of cellular physiology and metabolism. In Gram-positive bacteria such as B.
Danny K Fung +2 more
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Dual role of a (p)ppGpp‐ and (p)ppApp‐degrading enzyme in biofilm formation and interbacterial antagonism [PDF]
Molecular Microbiology, 2021 AbstractThe guanosine nucleotide‐based second messengers ppGpp and pppGpp (collectively: (p)ppGpp) enable adaptation of microorganisms to environmental changes and stress conditions. In contrast, the closely related adenosine nucleotides (p)ppApp are involved in type VI secretion system (T6SS)‐mediated killing during bacterial competition.
Wieland Steinchen +2 more
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Nonhydrolysable Analogues of (p)ppGpp and (p)ppApp Alarmone Nucleotides as Novel Molecular Tools [PDF]
ACS Chemical Biology, 2021 While alarmone nucleotides guanosine-3',5'-bisdiphosphate (ppGpp) and guanosine-5'-triphosphate-3'-diphosphate (pppGpp) are archetypical bacterial second messengers, their adenosine analogues ppApp (adenosine-3',5'-bisdiphosphate) and pppApp (adenosine-5'-triphosphate-3'-diphosphate) are toxic effectors that abrogate bacterial growth. The alarmones are
Viktor Mojr +2 more
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Emerging and divergent roles of pyrophosphorylated nucleotides in bacterial physiology and pathogenesis. [PDF]
PLoS Pathogens, 2021 Bacteria inhabit diverse environmental niches and consequently must modulate their metabolism to adapt to stress. The nucleotide second messengers guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp) (collectively referred to as (p ...
N Y Elizabeth Chau +3 more
doaj +2 more sources
How to quantify magic spots - a brief overview of (p)ppGpp detection and quantitation methods [PDF]
Frontiers in Molecular BiosciencesGuanosine tetra- and penta-phosphates, collectively known as (p)ppGpp, are well-known second messengers of cellular stress responses in bacteria and plants.
Filip Gąsior +2 more
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