Results 141 to 150 of about 41,121 (277)

PacBio Iso-Seq transcriptome sequencing of Diorhabda sublineata tissues

open access: yes, 2022
PacBio Iso-Seq transcriptome sequencing of Diorhabda sublineata (Mediterranean tamarisk beetle) head, thorax, and abdomen of three adult females was conducted by the USDA-ARS Ag100Pest ...
United States Department of Agriculture (17854922)
core  

Recent advances in the genomics of sugarcane (Saccharum)

open access: yesJournal of Integrative Plant Biology, EarlyView.
This review systematically summarizes advances in sugarcane genomics, evolution, classification, and breeding practices. It identifies key research bottlenecks and proposes an integrated research framework to facilitate predictive sugarcane breeding, which provides valuable references for genetic improvement of other complex polyploid crops.
Tianyou Wang   +5 more
wiley   +1 more source

T2T genome assembly and CRISPR/Cas9 editing of Medicago lupulina

open access: yesJournal of Integrative Plant Biology, EarlyView.
A complete, gap‐free genome of Medicago lupulina, a tough, high‐protein forage legume, and a gene‐editing tool for this species, which was used to alter its leaf shape, provide key resources for improving alfalfa and breeding better forage crops for high‐altitude environments.
Qing Yuan   +9 more
wiley   +1 more source

Unlocking the Potential of Metagenomics with the PacBio High-Fidelity Sequencing Technology

open access: yesMicroorganisms
Traditional methods for studying microbial communities have been limited due to difficulties in culturing and sequencing all microbial species. Recent advances in third-generation sequencing technologies, particularly PacBio’s high-fidelity (HiFi) sequencing, have significantly advanced metagenomics by providing accurate long-read sequences.
Yanhua Han   +7 more
openaire   +3 more sources

Assembly statistics per SMRT cell# for PacBio data using HGAP3.0 and Quiver.

open access: yes, 2019
Assembly statistics per SMRT cell# for PacBio data using HGAP3.0 and Quiver.
Narjol González-Escalona (280578)   +4 more
core   +1 more source

Crawling under the radar: Two novel Paulinella species expand knowledge about the ecology and evolution of a primary plastid‐containing amoeba lineage

open access: yesJournal of Phycology, EarlyView.
Abstract The genus Paulinella represents a rare, independent case of primary endosymbiosis, providing a unique system to study the early stages of organelle evolution. Here, we expand current understanding of primary plastid endosymbiosis through the discovery and characterization of two novel photosynthetic amoebae, Paulinella marae sp. nov.
Julia Van Etten   +9 more
wiley   +1 more source

Maize Genome Assembly with PacBio Reads

open access: yesBIO-PROTOCOL, 2022
Ying Hu, Marcio Resende Jr.
openaire   +1 more source

Genome‐scale insights into metabolic streamlining and photosynthetic energy balance in the extremophile green alga Picocystis salinarum (Picocystophyceae, Chlorophyta)

open access: yesJournal of Phycology, EarlyView.
Abstract Picocystis salinarum is an early‐diverging chlorophyte and the sole described member of the Picocystophyceae, frequently dominating hypersaline and alkaline lakes despite extreme physicochemical constraints. To elucidate the genomic foundations of its ecological success, we generated a fully annotated, chromosome‐scale nuclear genome assembly ...
Claude Lemieux   +2 more
wiley   +1 more source

Jointly benchmarking small and structural variant calls with vcfdist

open access: yesGenome Biology
In this work, we extend vcfdist to be the first variant call benchmarking tool to jointly evaluate phased single-nucleotide polymorphisms (SNPs), small insertions/deletions (INDELs), and structural variants (SVs) for the whole genome. First, we find that
Tim Dunn   +3 more
doaj   +1 more source

PBSIM3 simulated PacBio and ONT R10.4.1 reads for fusion transcripts

open access: yes
<p>Reads simulated for fusion transcripts using PBSIM3 based on PacBio and ONT R10.4.1 profiles.</p ...
Qin, Alvin, Haas, Brian, Popic, Victoria
core   +1 more source

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