Results 151 to 160 of about 47,490 (285)

Oxford Nanopore bests PacBio [PDF]

Nature Biotechnology, 2019
openaire   +1 more source

Genome and Single‐Cell Transcriptome Reveal the Evolution of Holoparasitic Plants: A Case Study of Cistanche deserticola

Plant Biotechnology Journal, EarlyView.
ABSTRACT The Orobanchaceae family, the largest group of parasitic plants, spans a complete spectrum from autotrophic to holoparasitic species. As a typical endangered holoparasitic species within this family, Cistanche deserticola is a parasitic plant that is widely harvested for traditional medicine in desertic regions, and of growing importance as a ...
Xinke Zhang, Yujing Miao, Yuanyuan Xing, Guoshuai Zhang, Meiqiao Zhang, Chris J. Thorogood, Shilin Chen, Linfang Huang   +7 more
wiley   +1 more source

Haplotype‐Resolved Genome Assembly and Population Genomics Reveal Evolutionary History and Agronomic Traits of Mulberry

Plant Biotechnology Journal, EarlyView.
ABSTRACT Mulberry is a representative economic tree species valued for both poverty alleviation and medicinal use. To advance the understanding of mulberry genomics and demography, we assembled high‐quality haploid genomes of two widely cultivated mulberry varieties NS14 and QS1, and analysed 376 accessions from 12 countries, including 39 ancient trees
Zhifeng Wang, Peigang Liu, Chengzhi Jiao, Yazhou Zhang, Zilong Xu, Tianbao Lin, Yan Zhu, Xin Jing, Yuan Huang, Nan Chao, Mengjie Zhao, Yan Liu, Huizi Liu, Bo Sun, Fen Zhang, Quanzi Li, Zhiqiang Lv, Xuepeng Sun, Ying‐Chung Jimmy Lin, Jia Wei   +19 more
wiley   +1 more source

Structural Variations Contribute to Subspeciation and Yield Heterosis in Rice

Plant Biotechnology Journal, EarlyView.
ABSTRACT Yield heterosis has been extensively exploited in hybrid breeding, with intersubspecific hybrids often exhibiting the most pronounced effects. However, developing elite hybrids remains a laborious and time‐consuming process. The genetic basis of heterosis has been debated for over a century, hindered largely by the lack of high‐quality genomes.
Zhiwu Dan, Yunping Chen, Wenchao Huang
wiley   +1 more source

DNA extraction protocol for yeast PacBio sequencing v1

, 2018
A very detailed four-day protocol for DNA extraction for yeast PacBio sequencing modified from Promega's protocol for yeast p13-14.
openaire   +1 more source

Two Indel Variations in the Regulated Carotenoid Pigment Gene Hold the Key to the Interspecies Flower Colour Differentiation in Liriodendron Plants

Plant Biotechnology Journal, EarlyView.
ABSTRACT Flower colour variation is pivotal for plant evolution, ecological adaptation, and pollinator attraction. In the Liriodendron genus, L. tulipifera features a distinct orange petal stripe which is absent in L. chinense, driven by differential carotenoid accumulation, yet the underlying genetic mechanism remains unclear.
Lichun Yang, Hainan Wu, Manli Bu, Huanhuan Liu, Yufang Shen, Hui Xia, Yaxian Zong, Jing Wang, Huogen Li   +8 more
wiley   +1 more source

Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in human microbiome samples

BMC Genomics
Background Sequencing variable regions of the 16S rRNA gene (≃300 bp) with Illumina technology is commonly used to study the composition of human microbiota.
Elena Buetas, Marta Jordán-López, Andrés López-Roldán, Giuseppe D’Auria, Llucia Martínez-Priego, Griselda De Marco, Miguel Carda-Diéguez, Alex Mira   +7 more
doaj   +1 more source

Pangenome Analysis Reveals Structural Variations Associated With Citric Acid Accumulation in Prunus mume

Plant Biotechnology Journal, EarlyView.
ABSTRACT Pangenome can reveal a large number of variations, providing a more comprehensive view of the genetic diversity of species that a single reference genome cannot surpass. Here, we assembled the haplotype telomere‐to‐telomere genome and 10 chromosome‐level genomes, integrated with two previously reported genomes, and constructed a graph ...
Xiao Huang, Ximeng Lin, Pengyu Zhou, Wei Tan, Feng Gao, Zhaojun Ni, Ting Shi, Yang Bai, Chengdong Ma, Yufan Ma, Minglu Li, Faisal Hayat, Qixiang Zhang, Ryutaro Tao, Jianmin Wan, Zhihong Gao   +15 more
wiley   +1 more source

Haploid Mutation Mapping Identifies a Homoeologous Non‐Reciprocal Translocation Linked to Reduced Fibre and Enhanced Protein in Brassica napus

Plant Biotechnology Journal, EarlyView.
ABSTRACT A key challenge for the genetic improvement of canola (Brassica napus), one of the world's most important oilseeds, is the limited natural variation for commercially important traits. The creation of new variation is hindered by the lack of functional knowledge about genes controlling these traits.
Morgan W. Kirzinger, Sarika Saini, Andrea T. Todd, Ushan Alahakoon, Kevin C. Koh, Justin B. Nichol, HaiYing Yuan, Kevin Fengler, Victor Llaca, Dustin Cram, Sampath Perumal, Wali Soomro, Magda Konopka, Tancey Melchkart, Venkat Bandi, Yasmina Bekkaoui, Yifang Tan, Chad Matsalla, Andrew G. Sharpe, Carl Gutwin, Fred Thoonen, Igor Falak, Chad Koscielny, Stuart Gardner, Isobel A. P. Parkin, Marcus A. Samuel, Alison M. R. Ferrie, Dave Charne, Daoquan Xiang, Jetty S. S. AmmiRaju, Sateesh Kagale   +30 more
wiley   +1 more source

P600: pb-StarPhase: A phase-aware pharmacogenomic diplotyper for long-read sequencing data

Genetics in Medicine Open
James Holt, John Harting, Nina Gonzaludo, Zev Kronenberg, Christopher Saunders, Michael Eberle   +5 more
doaj   +1 more source