Results 11 to 20 of about 47,490 (285)
PacBio Sequencing and Its Applications
Genomics, Proteomics & Bioinformatics, 2015 Single-molecule, real-time sequencing developed by Pacific BioSciences offers longer read lengths than the second-generation sequencing (SGS) technologies, making it well-suited for unsolved problems in genome, transcriptome, and epigenetics research ...
Anthony Rhoads, Kin Fai Au
doaj +3 more sources
PaSS: a sequencing simulator for PacBio sequencing [PDF]
BMC Bioinformatics, 2019 Background Third-generation sequencing platforms, such as PacBio sequencing, have been developed rapidly in recent years. PacBio sequencing generates much longer reads than the second-generation sequencing (or the next generation sequencing, NGS ...
Wenmin Zhang, Ben Jia, Chaochun Wei
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Large-scale and significant expression from pseudogenes in Sodalis glossinidius – a facultative bacterial endosymbiont [PDF]
, 2020 The majority of bacterial genomes have high coding efficiencies, but there are some genomes of intracellular bacteria that have low gene density. The genome of the endosymbiont Sodalis glossinidius contains almost 50 % pseudogenes containing mutations ...
Beynon, R +9 more
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HISEA: HIerarchical SEed Aligner for PacBio data [PDF]
BMC Bioinformatics, 2017 The next generation sequencing (NGS) techniques have been around for over a decade. Many of their fundamental applications rely on the ability to compute good genome assemblies. As the technology evolves, the assembly algorithms and tools have to continuously adjust and improve.
Nilesh Khiste, Lucian Ilie
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“polishCLR: a Nextflow workflow for polishing PacBio CLR genome assemblies” [PDF]
Genome Biology and Evolution, 2022 Abstract Long-read sequencing has revolutionized genome assembly, yielding highly contiguous, chromosome-level contigs. However, assemblies from some third generation long read technologies, such as Pacific Biosciences (PacBio) Continuous Long Reads (CLR), have a high error rate.
Jennifer Chang +5 more
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Single-molecule real-time sequencing combined with optical mapping yields completely finished fungal genome [PDF]
, 2015 Next-generation sequencing (NGS) technologies have increased the scalability, speed, and resolution of genomic sequencing and, thus, have revolutionized genomic studies.
Datema, Erwin +6 more
core +2 more sources
PACMAN: PacBio Methylation Analyzer
EMBnet.journal, 2015 We developed a tool written in Perl and an associated web site called PACMAN (PacBio Methylation Analyzer) allowing users to easily create images with Circos in a user-friendly interface. From the genome in FASTA format and the motifs in GFF format, PACMAN generates a publication quality image of the selected methylated motifs counts, locations and non-
Laurent Falquet, Alexis Loetscher
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Bi-Level Error Correction for PacBio Long Reads [PDF]
IEEE/ACM Transactions on Computational Biology and Bioinformatics, 2020 The latest sequencing technologies such as the Pacific Biosciences (PacBio) and Oxford Nanopore machines can generate long reads at the length of thousands of nucleic bases which is much longer than the reads at the length of hundreds generated by Illumina machines. However, these long reads are prone to much higher error rates, for example 15%, making
Yuansheng Liu +3 more
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Permanent draft genome sequence of Nocardia sp. BMG111209, an actinobacterium isolated from nodules of Casuarina glauca [PDF]
, 2016 Nocardia sp. strain BMG111209 is a non-Frankia actinobacterium isolated from root nodules of Casuarina glauca in Tunisia. Here, we report the 9.1-Mbp draft genome sequence of Nocardia sp.
Beauchemin, Nicholas +18 more
core +4 more sources
A Gapless, Unambiguous Genome Sequence of the Enterohemorrhagic Escherichia coli O157:H7 Strain EDL933. [PDF]
, 2014 Escherichia coli EDL933 is the prototypic strain for enterohemorrhagic E. coli serotype O157:H7, associated with deadly food-borne outbreaks. Because the publicly available sequence of the EDL933 genome has gaps and >6,000 ambiguous base calls, we ...
Aziz, Ramy K +4 more
core +2 more sources