Results 131 to 140 of about 14,181 (179)
Investigation of the active center of rat pancreatic elastase
BBA - Proteins and Proteomics, 1989 We have isolated rat pancreatic elastase I (EC 3.4.21.36) using a fast two-step procedure and we have investigated its active center with p-nitroanilide substrates and trifluoroacetylated inhibitors. These ligands were also used to probe porcine pancreatic elastase I whose amino acid sequence is 84% homologous to rat pancreatic elastase I as reported ...
Christian Boudier +2 more
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Digestion of elastin by porcine pancreatic elastase I and elastase II
International Journal of Peptide and Protein Research, 1985Elastin was fully solubilized by digestion with elastase I or elastase II. Each digest was separated into high‐molecular weight and low‐molecular weight fractions that were characterized by the correspondence to their amino acid content, N‐terminal sequence and C‐terminal amino acids. It was found that although the relative amount of amino acids in the
M, Vered, Y, Burstein, A, Gertler
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The effect of pancreatic elastase on diabetic nephropathy
Diabetes Research and Clinical Practice, 1994Pancreatic elastase has been shown to inhibit the thickening of glomerular basement membranes in experimental diabetic animals. We explored the clinical significance of the prolonged administration of pancreatic elastase on diabetic nephropathy, in patients whose blood glucose levels and blood pressure were controlled.
exaly +3 more sources
Purification and characterization of ovine pancreatic elastase
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 1998Elastase was isolated from ovine pancreas and purified to homogeneity by two different procedures. One involved precipitation with ammonium sulphate, p-aminobenzamidine-Sepharose chromatography, CM-Sepharose ion exchange chromatography and S-300 Sephacryl chromatography.
L S, Erlendsson, H, Filippusson
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Distribution of pancreatic elastase and metalloproteinase in vertebrates
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1986Elastase-like enzymes were detected as zymogens in all of the pancreatic extracts from the gummy shark, bullhead shark, angel shark, smooth hammerhead, bestel, rainbow trout, carp, eel, Japanese mackerel, yellowtail, sea bass, parrotfish, bullfrog, chicken, bluewhite dolphin, hog, rat, cat, and dog.
R, Yoshinaka +3 more
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Elastase Activity in Human Pancreatic Secretions
Nature, 1962IN 1949, Balo and Banga1 first reported the isolation of the pancreatic enzyme elastase, which was unique in its ability to render soluble elastin (an important structural component of arteries, veins, skin and lung). This enzyme was first believed to be mucolytic in action, but it is now established that it is also a potent proteolytic agent2.
S, TINDEL +3 more
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ELISA for human pancreatic elastase 1
Clinical Biochemistry, 1989A sandwich-type ELISA for human pancreatic elastase 1 was developed and assessed for clinical feasibility. Using highly purified reagents, the test seems to be as sensitive and specific as the well-documented Radio-immunoassay (RIA) for the detection of elevated elastase 1 serum levels and, consequently, would have the same indications described for ...
A, Sziegoleit, H, Knäpler, B, Peters
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Inhibition of Pancreatic Elastase by Polyphenolic Compounds
Journal of Agricultural and Food Chemistry, 2010Polyphenols are plant secondary metabolites commonly present in the human diet that possess the ability to bind and inhibit digestive proteins. In the present study, kinetic measurements of porcine pancreatic elastase (PPE) activity were determined using Suc-(Ala)(3)-p-nitroanilide as substrate and polyphenolic compounds as inhibitors.
Natercia F Bras +5 more
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Stabilization of Lyophilized Porcine Pancreatic Elastase
Pharmaceutical Research, 1993Porcine pancreatic elastase, a well-characterized serine protease, has been used as a model to assess the effects of excessive humidity on solid-state stability of the lyophilized protein. Elastase lyophilized without excipients retained full activity immediately after freeze-drying but became denatured upon continued storage at 40 degrees C, 75 ...
B S, Chang, C S, Randall, Y S, Lee
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Comparison of the Elastolytic Effects of Human Leukocyte Elastase and Porcine Pancreatic Elastase
Biochemical and Biophysical Research Communications, 1976Porcine and bovine elastins were digested by human leukocyte elastase and porcine pancreatic elastase. The enzymes showed similarities in the extent to which they digested elastin and the pattern and quantitative distribution of N-terminal amino acids in the digests.
R M, Senior +2 more
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