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Absolute Membrane Potential Recording with ASAP-Type Genetically Encoded Voltage Indicators Using Fluorescence Lifetime Imaging. [PDF]

open access: yesACS Chem Neurosci
Nair AG   +7 more
europepmc   +1 more source

Optical Recordings of Unitary Synaptic Connections Reveal High and Random Local Connectivity between CA3 Pyramidal Cells. [PDF]

open access: yesJ Neurosci
Layous R   +9 more
europepmc   +1 more source
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The Patch Clamp Technique

Neurosurgery, 1995
The introduction of the patch clamp technique less than two decades ago revolutionized the study of cellular physiology by providing a high-resolution method of observing the function of individual ionic channels in a variety of normal and pathological cell types.
L K, Liem   +3 more
openaire   +2 more sources

[Patch-clamp technique].

Yakubutsu, seishin, kodo = Japanese journal of psychopharmacology, 1992
We first describe how to set up the system for patch-clamp recording and carry out the experiments with 4 different modes of conventional patch-clamp technique: cell-attached, whole-cell, inside-out and outside-out configurations. Thereafter, we mention about an improved 'nystatin perforated' patch-clamp technique which dissolves the fault of ...
N, Akaike, T, Shirasaki
openaire   +1 more source

Patch-clamp technique

1995
In this chapter, I shall explain how to set-up a patch-clamp technique. The aim is to guide researchers in their initial steps in the field of electrophysiology, describing the instrumentation needed, as well as the technique itself. This chapter has been written assuming that the reader has a basic knowledge of electrophysiology.
openaire   +2 more sources

Conventional Micropipette-Based Patch Clamp Techniques

2013
The patch clamp technique revolutionized the study of ion channels and is considered the gold standard of measuring ion channel activity, from the academic laboratory to industrial-scale drug screening. This technique enables the study of ion channels, from single molecules up to the whole-cell ion channel population, and in their native environment ...
Jonathan D, Lippiat, David C, Wrighton
openaire   +2 more sources

Patch-clamp technique in renal physiology

American Journal of Physiology-Renal Physiology, 1986
The patch-clamp technique of Neher and Sakmann and their colleagues has been widely used over the last 5 years to investigate ion channels in excitable tissues. More recently, it has become useful as a tool to study channels involved in transepithelial ion transport.
openaire   +2 more sources

Patch clamp: non-traditional techniques

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 1988
Femtofarad changes in cell capacitance associated with exo- and endocytosis have been recorded. These measurements have generally confirmed electron-microscopic evidence of exocytotic vesicle size and have provided direct evidence for the intracellular fusion of secretory granules and a dynamic measurement of membrane trafficking.
openaire   +1 more source

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