Results 211 to 220 of about 39,769 (255)

Patch-clamp technique in renal physiology

American Journal of Physiology-Renal Physiology, 1986
The patch-clamp technique of Neher and Sakmann and their colleagues has been widely used over the last 5 years to investigate ion channels in excitable tissues. More recently, it has become useful as a tool to study channels involved in transepithelial ion transport.
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Patch clamp techniques: an overview.

Methods in enzymology, 1992
This chapter provides an overview of the patch clamp techniques. The patch clamp technique provides the experimental means for merging the tools of modern molecular and cellular biology with those of electrophysiology. Using various recording configurations, it is possible to dissect the mechanisms of channel modulation.
M, Cahalan, E, Neher
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[Patch-clamp technique].

Yakubutsu, seishin, kodo = Japanese journal of psychopharmacology, 1992
We first describe how to set up the system for patch-clamp recording and carry out the experiments with 4 different modes of conventional patch-clamp technique: cell-attached, whole-cell, inside-out and outside-out configurations. Thereafter, we mention about an improved 'nystatin perforated' patch-clamp technique which dissolves the fault of ...
N, Akaike, T, Shirasaki
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Perforated Patch-Clamp Technique

2003
Application of the conventional whole-cell patch-clamp method leads to a replacement of the intracellular fluid with the intracellular pipet solution. The speed of this replacement or dialysis depends on the cell volume and electrode tip diameter. Although this mechanism can be used advantageously in many experiments, there are conditions where such a ...
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Patch-Clamp Technique in ESC-Derived Cardiomyocytes

2014
ESC-derived cardiomyocytes are excitable cells that express many of the ion channels also found in adult cardiomyocytes. The patch-clamp technique is a powerful technique to characterize both the electrophysiological properties of excitable cells as well as the underlying ion channel currents responsible for these electrophysiological properties.
Jie, Liu, Peter H, Backx
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High-Resolution Patch-Clamp Techniques

1985
Ion channels are integral membrane proteins that catalyze the diffusion of ions across cell membranes. For example, a single ion channel may pass 20 pA of current, which is equivalent to a flow of ~108 ions/s. Conformational changes in the channel can turn this flow on and off, thereby producing pulses of current.
Anthony Auerbach, Frederick Sachs
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Patch-Clamp Technique in Brain Slices

2003
The technique of patch-clamp recording in brain slices is applicable to a large variety of cell types in slices from nearly all areas of the central nervous system (CNS) in animals at many different stages of development (Blanton et al., 1989; Edwards et al., 1989; Konnerth, 1990).
T. D. Plant, J. Eilers, A. Konnerth
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Patch Clamp Technique and Applications

2017
There are two main modes in patch clamp recordings: voltage clamp and current clamp. In the voltage clamp mode, the membrane voltage is controlled by the amplifier through the recording pipette and the corresponding current through the pipette is measured.
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Patch clamp: non-traditional techniques

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 1988
Femtofarad changes in cell capacitance associated with exo- and endocytosis have been recorded. These measurements have generally confirmed electron-microscopic evidence of exocytotic vesicle size and have provided direct evidence for the intracellular fusion of secretory granules and a dynamic measurement of membrane trafficking.
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Patch-Clamp Technique

2011
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