Optogenetics meets automated patch clamp [PDF]
iScienceSummary: Channelrhodopsins have revolutionized the rapid, contactless modulation of action potentials, driving major advances in both neuroscience and cardiac research.
Reetta Penttinen +9 more
doaj +2 more sources
A robotic patch-clamp system with real-time localization and phase-synchronized capture of dynamic in vivo cells using micropipette resistance modelling [PDF]
Microsystems & NanoengineeringIn vivo patch clamp is the gold standard for probing neuronal function in the intact brain of living animals through recordings of membrane potentials and picoampere-level ionic currents.
Ruimin Li +14 more
doaj +2 more sources
Patch-clamp amplifiers on a chip [PDF]
Journal of Neuroscience Methods, 2010 We present the first, fully integrated, two-channel implementation of a patch-clamp measurement system. With this "PatchChip" two simultaneous whole-cell recordings can be obtained with rms noise of 8pA in a 10kHz bandwidth. The capacitance and series-resistance of the electrode can be compensated up to 10pF and 100MΩ respectively under computer ...
Fred Sigworth +2 more
exaly +3 more sources
Robotic Fast Patch Clamp in Brain Slices Based on Stepwise Micropipette Navigation and Gigaseal Formation Control [PDF]
SensorsThe patch clamp technique has become the gold standard for neuron electrophysiology research in brain science. Brain slices have been widely utilized as the targets of the patch clamp technique due to their higher optical transparency compared to a live ...
Jinyu Qiu +5 more
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Progress in automating patch clamp cellular physiology
Brain and Neuroscience Advances, 2018 Patch clamp electrophysiology has transformed research in the life sciences over the last few decades. Since their inception, automatic patch clamp platforms have evolved considerably, demonstrating the capability to address both voltage- and ligand ...
Simon R Schultz, Luca Annecchino
exaly +2 more sources
Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections [PDF]
eLifeSignificant technical challenges exist when measuring synaptic connections between neurons in living brain tissue. The patch clamping technique, when used to probe for synaptic connections, is manually laborious and time-consuming.
Mighten C Yip +10 more
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Protocol for whole-cell patch-clamp assay for HBV-mediated sodium influx in THP-1 macrophages [PDF]
STAR ProtocolsSummary: Sodium influx induced by hepatitis B virus (HBV) enhances activation of the NLRP3 inflammasome, in synergy with potassium efflux signals in macrophages. Here, we present a protocol for conducting patch-clamp assays to measure HBV-mediated sodium
Yingbao Tan +3 more
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Immediate reuse of patch-clamp pipettes after ultrasonic cleaning [PDF]
Scientific ReportsThe patch-clamp technique has revolutionized neurophysiology by allowing to study single neuronal excitability, synaptic connectivity, morphology, and the transcriptomic profile.
Kevin Jehasse +6 more
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In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi [PDF]
Microsystems & NanoengineeringStudying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing pathogens.
Tanja Pajić +10 more
doaj +2 more sources
Frontiers in Molecular Neuroscience, 2022 Fluoride has been used in the internal recording solution for manual and automated patch clamp experiments for decades because it helps to improve the seal resistance and promotes longer lasting recordings.
Markus Rapedius +20 more
doaj +1 more source