Results 11 to 20 of about 326,331 (291)
History of Electrophysiology and the Patch Clamp
, 2014 We provide a historic outlook on the development of the concept of bioelectricity, with emphasis on the neuromuscular junction as a model that revolutionized our thinking of the nerve, nervous, and muscle tissue excitability. We abridge some crucial experiments in defining the electrical excitability of biological cells. We also provide an insight into
Verkhratsky, Alexei; id_orcid 0000-0003-2592-9898 +1 more
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Dendritic patch-clamp recording
Nature Protocols, 2006 The patch-clamp technique allows investigation of the electrical excitability of neurons and the functional properties and densities of ion channels. Most patch-clamp recordings from neurons have been made from the soma, the largest structure of individual neurons, while their dendrites, which form the majority of the surface area and receive most of ...
Davie, Jenny +6 more
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Bio-Protocol, 2021 The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and ...
Corey Landry +5 more
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Frontiers in Physiology, 2023 Advances in next-generation sequencing have been exceptionally valuable for identifying variants in medically actionable genes. However, for most missense variants there is insufficient evidence to permit definitive classification of variants as benign ...
Joanne G. Ma +5 more
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Patch-clamp studies of macroscopic and single-channel currents in mouse neuroblastoma cells.
, 1988 Cells from the clonal cell line N1E115, a mouse tumour of sympathetic origin, differentiate both morphologically and electrophysiologically when cultured in medium containing dimethylsulphoxide.
Rosalind Anita Jane. Smith (7657352)
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, 2021 Understanding of the relationship between cellular function and molecular composition holds a key to next-generation therapeutics but requires measurement of all types of molecules in cells.
Abigail M. Polter (11885712) +5 more
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Perforated Patch Clamp Recordings in ex vivo Brain Slices from Adult Mice
Bio-Protocol, 2023 Intracellular signaling pathways directly and indirectly regulate neuronal activity. In cellular electrophysiological measurements with sharp electrodes or whole-cell patch clamp recordings, there is a great risk that these signaling pathways are ...
Simon Hess +2 more
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Neuron Contact Detection Based on Pipette Precise Positioning for Robotic Brain-Slice Patch Clamps
Sensors, 2023 A patch clamp is the “gold standard” method for studying ion-channel biophysics and pharmacology. Due to the complexity of the operation and the heavy reliance on experimenter experience, more and more researchers are focusing on patch-clamp automation ...
Ke Li +6 more
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Biophysical Journal, 2002 To use one of the new benchtop patch-clamp systems, you first harvest the cells from a tissue-culture dish and wash by pelleting them in a microcentrifuge. Then you open the lid of the patch clamp box and pipette a few microliters of the cell suspension into the recording well. After a few minutes the cells settle onto the electrode array at the bottom
Sigworth, Fred J., Klemic, Kathryn G.
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Automatic deep learning-driven label-free image-guided patch clamp system
Nature Communications, 2021 Patch clamp recording of neurons is slow and labor-intensive. Here the authors present a method for automated deep learning driven label-free image guided patch clamp physiology to perform measurements on hundreds of human and rodent neurons.
Krisztian Koos +13 more
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