Results 41 to 50 of about 326,151 (193)

Transfection methods for high-throughput cellular assays of voltage-gated calcium and sodium channels involved in pain.

PLoS ONE, 2021
Chemical transfection is broadly used to transiently transfect mammalian cells, although often associated with cellular stress and membrane instability, which imposes challenges for most cellular assays, including high-throughput (HT) assays.
Md Mahadhi Hasan, Lotten Ragnarsson, Fernanda C Cardoso, Richard J Lewis   +3 more
doaj   +1 more source

A Temperature-Controlled Patch Clamp Platform Demonstrated on Jurkat T Lymphocytes and Human Induced Pluripotent Stem Cell-Derived Neurons

Bioengineering, 2020
Though patch clamping at room temperature is a widely disseminated standard procedure in the electrophysiological community, it does not represent the biological system in mammals at around 37 °C.
Jann Harberts, Max Kusch, John O’Sullivan, Robert Zierold, Robert H. Blick   +4 more
doaj   +1 more source

Scaled, high fidelity electrophysiological, morphological, and transcriptomic cell characterization

eLife, 2021
The Patch-seq approach is a powerful variation of the patch-clamp technique that allows for the combined electrophysiological, morphological, and transcriptomic characterization of individual neurons.
Brian R Lee, Agata Budzillo, Kristen Hadley, Jeremy A Miller, Tim Jarsky, Katherine Baker, DiJon Hill, Lisa Kim, Rusty Mann, Lindsay Ng, Aaron Oldre, Ram Rajanbabu, Jessica Trinh, Sara Vargas, Thomas Braun, Rachel A Dalley, Nathan W Gouwens, Brian E Kalmbach, Tae Kyung Kim, Kimberly A Smith, Gilberto Soler-Llavina, Staci Sorensen, Bosiljka Tasic, Jonathan T Ting, Ed Lein, Hongkui Zeng, Gabe J Murphy, Jim Berg   +27 more
doaj   +1 more source

Preparation of Giant Escherichia coli spheroplasts for Electrophysiological Recordings

Bio-Protocol, 2021
Prokaryotic ion channels have been instrumental in furthering our understanding of many fundamental aspects of ion channels’ structure and function. However, characterizing the biophysical properties of a prokaryotic ion channel in a native membrane ...
Yihao Jiang, Vinay Idikuda, Baron Chanda
doaj   +1 more source

A Hybrid Model for Safety Pharmacology on an Automated Patch Clamp Platform: Using Dynamic Clamp to Join iPSC-Derived Cardiomyocytes and Simulations of Ik1 Ion Channels in Real-Time

Frontiers in Physiology, 2018
An important aspect of the Comprehensive In Vitro Proarrhythmia Assay (CiPA) proposal is the use of human stem cell-derived cardiomyocytes and the confirmation of their predictive power in drug safety assays.
Birgit Goversen, Nadine Becker, Sonja Stoelzle-Feix, Alison Obergrussberger, Marc A. Vos, Toon A. B. van Veen, Niels Fertig, Teun P. de Boer   +7 more
doaj   +1 more source

Whole-cell and Perforated Patch-clamp Recordings from Acutely-isolated Murine Sino-atrial Node Cells

Bio-Protocol, 2020
Cardiac pacemaker cells of the sino-atrial node are responsible for the initiation of the heart beat and express an array of ion channels. The patch-clamp technique is the gold standard method for investigating the function of ion channels expressed in ...
Qadeer Aziz, Muriel Nobles, Andrew Tinker   +2 more
doaj   +1 more source

Computer modeling of whole-cell voltage-clamp analyses to delineate guidelines for good practice of manual and automated patch-clamp

Scientific Reports, 2021
The patch-clamp technique and more recently the high throughput patch-clamp technique have contributed to major advances in the characterization of ion channels.
Jérôme Montnach, Maxime Lorenzini, Adrien Lesage, Isabelle Simon, Sébastien Nicolas, Eléonore Moreau, Céline Marionneau, Isabelle Baró, Michel De Waard, Gildas Loussouarn   +9 more
doaj   +1 more source

Scorpion Venom peptide, AGAP inhibits TRPV1 and potentiates the analgesic effect of lidocaine

Heliyon, 2021
The current study was designed to test the hypothesis that BmK AGAP (AGAP) potentiates the analgesic effect of lidocaine. The chronic constrictive injury was performed on 72 rats to induce a rapid onset and long-lasting pain.
Sylvanus Kampo, Yong Cui, Jiachuan Yu, Thomas Winsum Anabah, Aglais Arredondo Falagán, Marcel Tunkumgnen Bayor, Qing-Ping Wen   +6 more
doaj   +1 more source

Exploring the Coordination of Cardiac Ion Channels With Action Potential Clamp Technique

Frontiers in Physiology, 2022
The patch clamp technique underwent continual advancement and developed numerous variants in cardiac electrophysiology since its introduction in the late 1970s. In the beginning, the capability of the technique was limited to recording one single current
Balázs Horváth, Norbert Szentandrássy, Norbert Szentandrássy, Csaba Dienes, Zsigmond M. Kovács, Péter P. Nánási, Péter P. Nánási, Ye Chen-Izu, Leighton T. Izu, Tamas Banyasz   +9 more
doaj   +1 more source

The application of the Escherichia coli giant spheroplast for drug screening with automated planar patch clamp system

Biotechnology Reports, 2015
Kv2.1, the voltage-gated ion channel, is ubiquitously expressed in variety of tissues and dysfunction of this ion channel is responsible for multiple diseases. Electrophysiological properties of ion channels are so far characterized with eukaryotic cells
Kyoko Kikuchi, Mika Sugiura, Chizuko Nishizawa-Harada, Tadashi Kimura   +3 more
doaj   +1 more source