Results 21 to 30 of about 326,151 (193)
In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi [PDF]
Microsystems & NanoengineeringStudying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing pathogens.
Tanja Pajić +10 more
doaj +2 more sources
Frontiers in Molecular Neuroscience, 2022 Fluoride has been used in the internal recording solution for manual and automated patch clamp experiments for decades because it helps to improve the seal resistance and promotes longer lasting recordings.
Markus Rapedius +20 more
doaj +1 more source
Bio-Protocol, 2021 The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and ...
Corey Landry +5 more
doaj +1 more source
Frontiers in Physiology, 2023 Advances in next-generation sequencing have been exceptionally valuable for identifying variants in medically actionable genes. However, for most missense variants there is insufficient evidence to permit definitive classification of variants as benign ...
Joanne G. Ma +5 more
doaj +1 more source
Patch-clamp studies of macroscopic and single-channel currents in mouse neuroblastoma cells.
, 1988 Cells from the clonal cell line N1E115, a mouse tumour of sympathetic origin, differentiate both morphologically and electrophysiologically when cultured in medium containing dimethylsulphoxide.
Rosalind Anita Jane. Smith (7657352)
core +6 more sources
, 2021 Understanding of the relationship between cellular function and molecular composition holds a key to next-generation therapeutics but requires measurement of all types of molecules in cells.
Abigail M. Polter (11885712) +5 more
core +2 more sources
Perforated Patch Clamp Recordings in ex vivo Brain Slices from Adult Mice
Bio-Protocol, 2023 Intracellular signaling pathways directly and indirectly regulate neuronal activity. In cellular electrophysiological measurements with sharp electrodes or whole-cell patch clamp recordings, there is a great risk that these signaling pathways are ...
Simon Hess +2 more
doaj +1 more source
Neuron Contact Detection Based on Pipette Precise Positioning for Robotic Brain-Slice Patch Clamps
Sensors, 2023 A patch clamp is the “gold standard” method for studying ion-channel biophysics and pharmacology. Due to the complexity of the operation and the heavy reliance on experimenter experience, more and more researchers are focusing on patch-clamp automation ...
Ke Li +6 more
doaj +1 more source
Biophysical Journal, 2002 To use one of the new benchtop patch-clamp systems, you first harvest the cells from a tissue-culture dish and wash by pelleting them in a microcentrifuge. Then you open the lid of the patch clamp box and pipette a few microliters of the cell suspension into the recording well. After a few minutes the cells settle onto the electrode array at the bottom
Sigworth, Fred J., Klemic, Kathryn G.
openaire +2 more sources
Automatic deep learning-driven label-free image-guided patch clamp system
Nature Communications, 2021 Patch clamp recording of neurons is slow and labor-intensive. Here the authors present a method for automated deep learning driven label-free image guided patch clamp physiology to perform measurements on hundreds of human and rodent neurons.
Krisztian Koos +13 more
doaj +1 more source