Results 11 to 20 of about 326,151 (193)

Progress in automating patch clamp cellular physiology [PDF]

Brain and Neuroscience Advances, 2018
Patch clamp electrophysiology has transformed research in the life sciences over the last few decades. Since their inception, automatic patch clamp platforms have evolved considerably, demonstrating the capability to address both voltage- and ligand ...
Simon R Schultz, Luca Annecchino
exaly   +3 more sources

Optogenetics meets automated patch clamp [PDF]

iScience
Summary: Channelrhodopsins have revolutionized the rapid, contactless modulation of action potentials, driving major advances in both neuroscience and cardiac research.
Reetta Penttinen, Ariel Coli, Florian Hintermaier, Nicoletta Murciano, Stephan Holzhauser, Shiqiang Gao, Maria Giustina Rotordam, Michael George, Niels Fertig, Lars Kaestner   +9 more
doaj   +2 more sources

A robotic patch-clamp system with real-time localization and phase-synchronized capture of dynamic in vivo cells using micropipette resistance modelling [PDF]

Microsystems & Nanoengineering
In vivo patch clamp is the gold standard for probing neuronal function in the intact brain of living animals through recordings of membrane potentials and picoampere-level ionic currents.
Ruimin Li, Hao Chen, Bo Hu, Jinyu Qiu, Yuzhu Liu, Zijian Guo, Shouguo Wu, Mengya Liu, Ran Ding, Shengkai Ding, Yuanyuan Li, Xiaogang Pang, Hui Shen, Qili Zhao, Xin Zhao   +14 more
doaj   +2 more sources

Patch-clamp amplifiers on a chip [PDF]

Journal of Neuroscience Methods, 2010
We present the first, fully integrated, two-channel implementation of a patch-clamp measurement system. With this "PatchChip" two simultaneous whole-cell recordings can be obtained with rms noise of 8pA in a 10kHz bandwidth. The capacitance and series-resistance of the electrode can be compensated up to 10pF and 100MΩ respectively under computer ...
Fred Sigworth, Joseph Santos-Sacchi, Eugenio Culurciello   +2 more
exaly   +3 more sources

Robotic Fast Patch Clamp in Brain Slices Based on Stepwise Micropipette Navigation and Gigaseal Formation Control [PDF]

Sensors
The patch clamp technique has become the gold standard for neuron electrophysiology research in brain science. Brain slices have been widely utilized as the targets of the patch clamp technique due to their higher optical transparency compared to a live ...
Jinyu Qiu, Qili Zhao, Ruimin Li, Yuzhu Liu, Biting Ma, Xin Zhao   +5 more
doaj   +2 more sources

History of Electrophysiology and the Patch Clamp

, 2014
We provide a historic outlook on the development of the concept of bioelectricity, with emphasis on the neuromuscular junction as a model that revolutionized our thinking of the nerve, nervous, and muscle tissue excitability. We abridge some crucial experiments in defining the electrical excitability of biological cells. We also provide an insight into
Verkhratsky, Alexei; id_orcid 0000-0003-2592-9898, Parpura, Vladimir   +1 more
openaire   +3 more sources

Dendritic patch-clamp recording

Nature Protocols, 2006
The patch-clamp technique allows investigation of the electrical excitability of neurons and the functional properties and densities of ion channels. Most patch-clamp recordings from neurons have been made from the soma, the largest structure of individual neurons, while their dendrites, which form the majority of the surface area and receive most of ...
Davie, Jenny, Kole, Maarten, Letzkus, Johannes, Rancz, Ede A, Spruston, Nelson, Stuart, Gregory J, Hausser, Michael   +6 more
openaire   +5 more sources

Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections [PDF]

eLife
Significant technical challenges exist when measuring synaptic connections between neurons in living brain tissue. The patch clamping technique, when used to probe for synaptic connections, is manually laborious and time-consuming.
Mighten C Yip, Mercedes M Gonzalez, Colby F Lewallen, Corey R Landry, Ilya Kolb, Bo Yang, William M Stoy, Ming-fai Fong, Matthew JM Rowan, Edward S Boyden, Craig R Forest   +10 more
doaj   +2 more sources

Protocol for whole-cell patch-clamp assay for HBV-mediated sodium influx in THP-1 macrophages [PDF]

STAR Protocols
Summary: Sodium influx induced by hepatitis B virus (HBV) enhances activation of the NLRP3 inflammasome, in synergy with potassium efflux signals in macrophages. Here, we present a protocol for conducting patch-clamp assays to measure HBV-mediated sodium
Yingbao Tan, Penghui Chen, Xiaojuan Xin, Jingxue Wang   +3 more
doaj   +2 more sources

Immediate reuse of patch-clamp pipettes after ultrasonic cleaning [PDF]

Scientific Reports
The patch-clamp technique has revolutionized neurophysiology by allowing to study single neuronal excitability, synaptic connectivity, morphology, and the transcriptomic profile.
Kevin Jehasse, Jean-Sébastien Jouhanneau, Sophie Wetz, Alexander Schwedt, James F. A. Poulet, Peter Neumann-Raizel, Björn M. Kampa   +6 more
doaj   +2 more sources