Results 1 to 10 of about 338,917 (289)

Progress in automating patch clamp cellular physiology [PDF]

Brain and Neuroscience Advances, 2018
Patch clamp electrophysiology has transformed research in the life sciences over the last few decades. Since their inception, automatic patch clamp platforms have evolved considerably, demonstrating the capability to address both voltage- and ligand ...
Simon R Schultz, Luca A Annecchino
exaly   +3 more sources

Immediate reuse of patch-clamp pipettes after ultrasonic cleaning [PDF]

Scientific Reports
The patch-clamp technique has revolutionized neurophysiology by allowing to study single neuronal excitability, synaptic connectivity, morphology, and the transcriptomic profile.
Kevin Jehasse, Jean-Sébastien Jouhanneau, Sophie Wetz, Alexander Schwedt, James F. A. Poulet, Peter Neumann-Raizel, Björn M. Kampa   +6 more
doaj   +4 more sources

Optogenetics meets automated patch clamp [PDF]

iScience
Summary: Channelrhodopsins have revolutionized the rapid, contactless modulation of action potentials, driving major advances in both neuroscience and cardiac research.
Reetta Penttinen, Ariel Coli, Florian Hintermaier, Nicoletta Murciano, Stephan Holzhauser, Shiqiang Gao, Maria Giustina Rotordam, Michael George, Niels Fertig, Lars Kaestner   +9 more
doaj   +2 more sources

There is no F in APC: Using physiological fluoride-free solutions for high throughput automated patch clamp experiments

Frontiers in Molecular Neuroscience, 2022
Fluoride has been used in the internal recording solution for manual and automated patch clamp experiments for decades because it helps to improve the seal resistance and promotes longer lasting recordings.
Alison Obergrussberger, Niels Fertig, Markus Rapedius   +2 more
exaly   +3 more sources

A robotic patch-clamp system with real-time localization and phase-synchronized capture of dynamic in vivo cells using micropipette resistance modelling [PDF]

Microsystems & Nanoengineering
In vivo patch clamp is the gold standard for probing neuronal function in the intact brain of living animals through recordings of membrane potentials and picoampere-level ionic currents.
Ruimin Li, Hao Chen, Bo Hu, Jinyu Qiu, Yuzhu Liu, Zijian Guo, Shouguo Wu, Mengya Liu, Ran Ding, Shengkai Ding, Yuanyuan Li, Xiaogang Pang, Hui Shen, Qili Zhao, Xin Zhao   +14 more
doaj   +2 more sources

Robotic Fast Patch Clamp in Brain Slices Based on Stepwise Micropipette Navigation and Gigaseal Formation Control [PDF]

Sensors
The patch clamp technique has become the gold standard for neuron electrophysiology research in brain science. Brain slices have been widely utilized as the targets of the patch clamp technique due to their higher optical transparency compared to a live ...
Jinyu Qiu, Qili Zhao, Ruimin Li, Yuzhu Liu, Biting Ma, Xin Zhao   +5 more
doaj   +2 more sources

Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections [PDF]

eLife
Significant technical challenges exist when measuring synaptic connections between neurons in living brain tissue. The patch clamping technique, when used to probe for synaptic connections, is manually laborious and time-consuming.
Mighten C Yip, Mercedes M Gonzalez, Colby F Lewallen, Corey R Landry, Ilya Kolb, Bo Yang, William M Stoy, Ming-fai Fong, Matthew JM Rowan, Edward S Boyden, Craig R Forest   +10 more
doaj   +2 more sources

Protocol for whole-cell patch-clamp assay for HBV-mediated sodium influx in THP-1 macrophages [PDF]

STAR Protocols
Summary: Sodium influx induced by hepatitis B virus (HBV) enhances activation of the NLRP3 inflammasome, in synergy with potassium efflux signals in macrophages. Here, we present a protocol for conducting patch-clamp assays to measure HBV-mediated sodium
Yingbao Tan, Penghui Chen, Xiaojuan Xin, Jingxue Wang   +3 more
doaj   +2 more sources

In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi [PDF]

Microsystems & Nanoengineering
Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing pathogens.
Tanja Pajić, Katarina Stevanović, Nataša V. Todorović, Aleksandar J. Krmpot, Miroslav Živić, Svetlana Savić-Šević, Steva M. Lević, Marina Stanić, Dejan Pantelić, Brana Jelenković, Mihailo D. Rabasović   +10 more
doaj   +2 more sources

Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts

Bio-Protocol, 2021
The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and ...
Corey Landry, Mighten Yip, Ilya Kolb, William Stoy, Mercedes Gonzalez, Craig Forest   +5 more
doaj   +1 more source