Results 1 to 10 of about 1,044,066 (265)

Immediate reuse of patch-clamp pipettes after ultrasonic cleaning [PDF]

Scientific Reports
The patch-clamp technique has revolutionized neurophysiology by allowing to study single neuronal excitability, synaptic connectivity, morphology, and the transcriptomic profile.
Kevin Jehasse, Jean-Sébastien Jouhanneau, Sophie Wetz, Alexander Schwedt, James F. A. Poulet, Peter Neumann-Raizel, Björn M. Kampa   +6 more
doaj   +3 more sources

State-of-the-art automated patch clamp devices: Heat activation, action potentials and high throughput in ion channel screening

Frontiers in Pharmacology, 2011
Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery.
Sonja eStoelzle, Alison eObergrussberger, Andrea eBruggemann, Claudia eHaarmann, Michael eGeorge, Ralf Franz Kettenhofen, Niels eFertig   +6 more
doaj   +1 more source

High-throughput electrophysiological assays for voltage gated ion channels using SyncroPatch 768PE. [PDF]

PLoS ONE, 2017
Ion channels regulate a variety of physiological processes and represent an important class of drug target. Among the many methods of studying ion channel function, patch clamp electrophysiology is considered the gold standard by providing the ultimate ...
Tianbo Li, Gang Lu, Eugene Y Chiang, Tania Chernov-Rogan, Jane L Grogan, Jun Chen   +5 more
doaj   +1 more source

Blebbistatin protects iPSC-CMs from hypercontraction and facilitates automated patch-clamp based electrophysiological study

Stem Cell Research, 2021
Recently, there have been great advances in cardiovascular channelopathy modeling and drug safety pharmacology using human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs).
Wener Li, Xiaojing Luo, Ying Ulbricht, Kaomei Guan   +3 more
doaj   +1 more source

A Short Guide to Electrophysiology and Ion Channels

Journal of Pharmacy & Pharmaceutical Sciences, 2017
The birth and discovery of electrophysiological science took place in the 18­­th century laying the path for our understanding of nerve membrane ionic currents.
Hussein Nori Rubaiy
doaj   +1 more source

High-throughput evaluation of epilepsy-associated KCNQ2 variants reveals functional and pharmacological heterogeneity

JCI Insight, 2022
Hundreds of genetic variants in KCNQ2 encoding the voltage-gated potassium channel KV7.2 are associated with early onset epilepsy and/or developmental disability, but the functional consequences of most variants are unknown.
Carlos G. Vanoye, Reshma R. Desai, Zhigang Ji, Sneha Adusumilli, Nirvani Jairam, Nora Ghabra, Nishtha Joshi, Eryn Fitch, Katherine L. Helbig, Dianalee McKnight, Amanda S. Lindy, Fanggeng Zou, Ingo Helbig, Edward C. Cooper, Alfred L. George Jr.   +14 more
doaj   +1 more source

From understanding cellular function to novel drug discovery: the role of planar patch-clamp array chip technology

Frontiers in Pharmacology, 2011
All excitable cell functions rely upon ion channels that are embedded in their plasma membrane. Perturbations of ion channel structure or function result in pathologies ranging from cardiac dysfunction to neurodegenerative disorders.
Christophe ePy, Marzia eMartina, Gerardo A Diaz-Quijada, Collin C Luk, Dolores eMartinez, Mike W Denhoff, Anne eCharrier, Tanya eComas, Robert eMonette, Anthony eKrantis, Naweed I Syed, Geoffrey A.R. Mealing   +11 more
doaj   +1 more source

High throughput clone screening on overexpressed hERG1 and Kv1.3 potassium channels using ion channel reader (ICR) label free technology

Heliyon, 2023
Pharmacological studies aimed at the development of newly synthesized drugs directed against ion channels (as well as genetic studies of ion channel mutations) involve the development and use of transfected cells.
Alberto Montalbano, Cesare Sala, Ginevra Chioccioli Altadonna, Andrea Becchetti, Annarosa Arcangeli   +4 more
doaj   +1 more source

A range of voltage-clamp protocol designs for rapid capture of hERG kinetics [version 2; peer review: 3 approved]

Wellcome Open Research
We provide details of a series of short voltage-clamp protocols designed for gathering a large amount of information on hERG (Kv11.1) ion channel gating.
Chon Lok Lei, Dominic J Whittaker, Matthew D Perry, Monique J Windley, Gary R Mirams, Adam P Hill   +5 more
doaj   +1 more source

ACQ4: an open-source software platform for data acquisition and analysis in neurophysiology research

Frontiers in Neuroinformatics, 2014
The complexity of modern neurophysiology experiments requires specialized software to coordinate multiple acquisition devices and analyze the collected data.
Luke eCampagnola, Megan B. Kratz, Paul B. Manis   +2 more
doaj   +1 more source