A modern automated patch-clamp approach for high throughput electrophysiology recordings in native cardiomyocytes [PDF]
Communications Biology, 2022 Crucial conventional patch-clamp approaches to investigate cellular electrophysiology suffer from low-throughput and require considerable experimenter expertise.
Fitzwilliam Seibertz +10 more
doaj +2 more sources
Adventures and Advances in Time Travel With Induced Pluripotent Stem Cells and Automated Patch Clamp [PDF]
Frontiers in Molecular Neuroscience, 2022 In the Hollywood blockbuster “The Curious Case of Benjamin Button” a fantastical fable unfolds of a man’s life that travels through time reversing the aging process; as the tale progresses, the frail old man becomes a vigorous, vivacious young man, then ...
Kadla R. Rosholm +9 more
doaj +2 more sources
A Hybrid Model for Safety Pharmacology on an Automated Patch Clamp Platform: Using Dynamic Clamp to Join iPSC-Derived Cardiomyocytes and Simulations of Ik1 Ion Channels in Real-Time [PDF]
Frontiers in Physiology, 2018 An important aspect of the Comprehensive In Vitro Proarrhythmia Assay (CiPA) proposal is the use of human stem cell-derived cardiomyocytes and the confirmation of their predictive power in drug safety assays.
Birgit Goversen +7 more
doaj +2 more sources
Development of ASIC1a ligand-gated ion channel drug screening assays across multiple automated patch clamp platforms [PDF]
Frontiers in Molecular Neuroscience, 2022 Human acid-sensing ion channels (ASIC) are ligand-gated ionotropic receptors expressed widely in peripheral tissues as well as sensory and central neurons and implicated in detection of inflammation, tissue injury, and hypoxia-induced acidosis.
John Ridley +8 more
doaj +2 more sources
Automated Planar Patch-Clamp Recording of P2X Receptors [PDF]
, 2019 P2X receptors are a structurally and functionally distinctive family of ligand-gated ion channels that play important roles in mediating extracellular adenosine 5'-triphosphate (ATP) signaling in diverse physiological and pathophysiological processes.
Milligan, CJ, Jiang, L-H
openaire +6 more sources
Computer modeling of whole-cell voltage-clamp analyses to delineate guidelines for good practice of manual and automated patch-clamp [PDF]
Scientific Reports, 2021 The patch-clamp technique and more recently the high throughput patch-clamp technique have contributed to major advances in the characterization of ion channels.
Jérôme Montnach +9 more
doaj +2 more sources
Stem Cell Research, 2019 Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have evolved into widely used and reliable cell sources for modeling cardiovascular channelopathies and for drug safety pharmacology.
Wener Li +6 more
doaj +3 more sources
Deep Learning‐Based Ion Channel Kinetics Analysis for Automated Patch Clamp Recording [PDF]
Advanced ScienceThe patch clamp technique is a fundamental tool for investigating ion channel dynamics and electrophysiological properties. This study proposes the first artificial intelligence framework for characterizing multiple ion channel kinetics of whole‐cell ...
Shengjie Yang +7 more
doaj +2 more sources
Development of an Automated Cell-Based Assay for the Detection of the Functional Activity of Saxitoxin [PDF]
ToxinsSaxitoxin (STX) is one of the most potent natural neurotoxins known and is the only marine toxin to be declared a chemical weapon. In both marine and freshwater systems filter feeding organisms can accumulate saxitoxin and human consumption of toxin ...
Rachel Whiting +5 more
doaj +2 more sources
Automated Patch-Clamp and Induced Pluripotent Stem Cell-Derived Cardiomyocytes: A Synergistic Approach in the Study of Brugada Syndrome. [PDF]
Int J Mol Sci, 2023 The development of high-throughput automated patch-clamp technology is a recent breakthrough in the field of Brugada syndrome research. Brugada syndrome is a heart disorder marked by abnormal electrocardiographic readings and an elevated risk of sudden ...
Melgari D +6 more
europepmc +3 more sources