There is no F in APC: Using physiological fluoride-free solutions for high throughput automated patch clamp experiments [PDF]
Frontiers in Molecular Neuroscience, 2022 Fluoride has been used in the internal recording solution for manual and automated patch clamp experiments for decades because it helps to improve the seal resistance and promotes longer lasting recordings.
Markus Rapedius +20 more
doaj +5 more sources
Development of automated patch clamp assays to overcome the burden of variants of uncertain significance in inheritable arrhythmia syndromes [PDF]
Frontiers in Physiology, 2023 Advances in next-generation sequencing have been exceptionally valuable for identifying variants in medically actionable genes. However, for most missense variants there is insufficient evidence to permit definitive classification of variants as benign ...
Joanne G. Ma +5 more
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Early identification of hERG liability in drug discovery programs by automated patch clamp [PDF]
Frontiers in Pharmacology, 2014 Blockade of the cardiac ion channel coded by hERG can lead to cardiac arrhythmia, which has become a major concern in drug discovery and development.
Timm eDanker, Clemens eMöller
doaj +6 more sources
Optogenetics meets automated patch clamp [PDF]
iScienceSummary: Channelrhodopsins have revolutionized the rapid, contactless modulation of action potentials, driving major advances in both neuroscience and cardiac research.
Reetta Penttinen +9 more
doaj +6 more sources
Progress in automating patch clamp cellular physiology [PDF]
Brain and Neuroscience Advances, 2018 Patch clamp electrophysiology has transformed research in the life sciences over the last few decades. Since their inception, automatic patch clamp platforms have evolved considerably, demonstrating the capability to address both voltage- and ligand ...
Luca A. Annecchino, Simon R. Schultz
doaj +5 more sources
Biophysical characterization of light-gated ion channels using planar automated patch clamp [PDF]
Frontiers in Molecular Neuroscience, 2022 Channelrhodopsins (ChRs) are proteins that guide phototaxis in protists and exhibit light-gated channel conductance when their genes are heterologously expressed in mammalian cells.
Elena G. Govorunova +3 more
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Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples [PDF]
Marine DrugsTetrodotoxin (TTX) is a marine toxin responsible for many intoxications around the world. Its presence in some pufferfish species and, as recently reported, in shellfish, poses a serious health concern. Although TTX is not routinely monitored, there is a
Mònica Campàs +5 more
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An Advanced Automated Patch Clamp Protocol Design to Investigate Drug—Ion Channel Binding Dynamics [PDF]
Frontiers in Pharmacology, 2021 Standard high throughput screening projects using automated patch-clamp instruments often fail to grasp essential details of the mechanism of action, such as binding/unbinding dynamics and modulation of gating.
Peter Lukacs +10 more
doaj +2 more sources
Biotechnology Reports, 2015 Kv2.1, the voltage-gated ion channel, is ubiquitously expressed in variety of tissues and dysfunction of this ion channel is responsible for multiple diseases. Electrophysiological properties of ion channels are so far characterized with eukaryotic cells
Kyoko Kikuchi +3 more
doaj +2 more sources
From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom research [PDF]
Frontiers in Pharmacology, 2023 Ion channels play a crucial role in diverse physiological processes, including neurotransmission and muscle contraction. Venomous creatures exploit the vital function of ion channels by producing toxins in their venoms that specifically target these ion ...
Shirin Ahmadi +8 more
doaj +2 more sources