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Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples [PDF]
Marine DrugsTetrodotoxin (TTX) is a marine toxin responsible for many intoxications around the world. Its presence in some pufferfish species and, as recently reported, in shellfish, poses a serious health concern. Although TTX is not routinely monitored, there is a
Mònica Campàs +5 more
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Stem Cell Research, 2019 Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have evolved into widely used and reliable cell sources for modeling cardiovascular channelopathies and for drug safety pharmacology.
Wener Li +6 more
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Development of ASIC1a ligand-gated ion channel drug screening assays across multiple automated patch clamp platforms [PDF]
Frontiers in Molecular Neuroscience, 2022 Human acid-sensing ion channels (ASIC) are ligand-gated ionotropic receptors expressed widely in peripheral tissues as well as sensory and central neurons and implicated in detection of inflammation, tissue injury, and hypoxia-induced acidosis.
John Ridley +8 more
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Computer modeling of whole-cell voltage-clamp analyses to delineate guidelines for good practice of manual and automated patch-clamp [PDF]
Scientific Reports, 2021 The patch-clamp technique and more recently the high throughput patch-clamp technique have contributed to major advances in the characterization of ion channels.
Jérôme Montnach +9 more
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Deep Learning‐Based Ion Channel Kinetics Analysis for Automated Patch Clamp Recording [PDF]
Advanced ScienceThe patch clamp technique is a fundamental tool for investigating ion channel dynamics and electrophysiological properties. This study proposes the first artificial intelligence framework for characterizing multiple ion channel kinetics of whole‐cell ...
Shengjie Yang +7 more
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Stem Cell Research, 2021 Recently, there have been great advances in cardiovascular channelopathy modeling and drug safety pharmacology using human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs).
Wener Li +3 more
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Development of an Automated Cell-Based Assay for the Detection of the Functional Activity of Saxitoxin [PDF]
ToxinsSaxitoxin (STX) is one of the most potent natural neurotoxins known and is the only marine toxin to be declared a chemical weapon. In both marine and freshwater systems filter feeding organisms can accumulate saxitoxin and human consumption of toxin ...
Rachel Whiting +5 more
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Neuron Contact Detection Based on Pipette Precise Positioning for Robotic Brain-Slice Patch Clamps
Sensors, 2023 A patch clamp is the “gold standard” method for studying ion-channel biophysics and pharmacology. Due to the complexity of the operation and the heavy reliance on experimenter experience, more and more researchers are focusing on patch-clamp automation ...
Ke Li +6 more
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Wellcome Open Research, 2021 Automated patch-clamp platforms are widely used and vital tools in both academia and industry to enable high-throughput studies such as drug screening. A leak current to ground occurs whenever the seal between a pipette and cell (or internal solution and
Chon Lok Lei +7 more
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Automatic deep learning-driven label-free image-guided patch clamp system
Nature Communications, 2021 Patch clamp recording of neurons is slow and labor-intensive. Here the authors present a method for automated deep learning driven label-free image guided patch clamp physiology to perform measurements on hundreds of human and rodent neurons.
Krisztian Koos +13 more
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