Results 21 to 30 of about 1,031,034 (270)

A modern automated patch-clamp approach for high throughput electrophysiology recordings in native cardiomyocytes [PDF]

Communications Biology, 2022
Crucial conventional patch-clamp approaches to investigate cellular electrophysiology suffer from low-throughput and require considerable experimenter expertise.
Fitzwilliam Seibertz, Markus Rapedius, Funsho E. Fakuade, Philipp Tomsits, Aiste Liutkute, Lukas Cyganek, Nadine Becker, Rupamanjari Majumder, Sebastian Clauß, Niels Fertig, Niels Voigt   +10 more
doaj   +2 more sources

Adventures and Advances in Time Travel With Induced Pluripotent Stem Cells and Automated Patch Clamp [PDF]

Frontiers in Molecular Neuroscience, 2022
In the Hollywood blockbuster “The Curious Case of Benjamin Button” a fantastical fable unfolds of a man’s life that travels through time reversing the aging process; as the tale progresses, the frail old man becomes a vigorous, vivacious young man, then ...
Kadla R. Rosholm, Beatrice Badone, Stefania Karatsiompani, David Nagy, Fitzwilliam Seibertz, Fitzwilliam Seibertz, Niels Voigt, Niels Voigt, Niels Voigt, Damian C. Bell   +9 more
doaj   +2 more sources

Automated Planar Patch-Clamp Recording of P2X Receptors [PDF]

, 2019
P2X receptors are a structurally and functionally distinctive family of ligand-gated ion channels that play important roles in mediating extracellular adenosine 5'-triphosphate (ATP) signaling in diverse physiological and pathophysiological processes.
Milligan, CJ, Jiang, L-H
openaire   +6 more sources

A Hybrid Model for Safety Pharmacology on an Automated Patch Clamp Platform: Using Dynamic Clamp to Join iPSC-Derived Cardiomyocytes and Simulations of Ik1 Ion Channels in Real-Time [PDF]

Frontiers in Physiology, 2018
An important aspect of the Comprehensive In Vitro Proarrhythmia Assay (CiPA) proposal is the use of human stem cell-derived cardiomyocytes and the confirmation of their predictive power in drug safety assays.
Birgit Goversen, Nadine Becker, Sonja Stoelzle-Feix, Alison Obergrussberger, Marc A. Vos, Toon A. B. van Veen, Niels Fertig, Teun P. de Boer   +7 more
doaj   +2 more sources

Immediate reuse of patch-clamp pipettes after ultrasonic cleaning [PDF]

Scientific Reports
The patch-clamp technique has revolutionized neurophysiology by allowing to study single neuronal excitability, synaptic connectivity, morphology, and the transcriptomic profile.
Kevin Jehasse, Jean-Sébastien Jouhanneau, Sophie Wetz, Alexander Schwedt, James F. A. Poulet, Peter Neumann-Raizel, Björn M. Kampa   +6 more
doaj   +3 more sources

Automated Patch-Clamp and Induced Pluripotent Stem Cell-Derived Cardiomyocytes: A Synergistic Approach in the Study of Brugada Syndrome. [PDF]

Int J Mol Sci, 2023
The development of high-throughput automated patch-clamp technology is a recent breakthrough in the field of Brugada syndrome research. Brugada syndrome is a heart disorder marked by abnormal electrocardiographic readings and an elevated risk of sudden ...
Melgari D, Calamaio S, Frosio A, Prevostini R, Anastasia L, Pappone C, Rivolta I.   +6 more
europepmc   +3 more sources

Development of Automated Patch Clamp Technique to Investigate CFTR Chloride Channel Function. [PDF]

Front Pharmacol, 2017
International audienceThe chloride (Cl-) channel cystic fibrosis transmembrane conductance regulator (CFTR) is defective in cystic fibrosis (CF), and mutation of its encoding gene leads to various defects such as retention of the misfolded protein in the
Billet A, Froux L, Hanrahan JW, Becq F.
europepmc   +2 more sources

Force-controlled patch clamp of beating cardiac cells. [PDF]

, 2015
From its invention in the 1970s, the patch clamp technique is the gold standard in electrophysiology research and drug screening because it is the only tool enabling accurate investigation of voltage-gated ion channels, which are responsible for action ...
Mohamed-Yassine Amarouch (1640302), János Vörös (1640305), Amarouch, Mohamed Yassine, Ossola, Dario, Pascal Behr (1640299), Dario Ossola (293103), Behr, Pascal, Vörös, János, Abriel, Hugues, Hugues Abriel (149294), Tomaso Zambelli (293109), Zambelli, Tomaso, Amarouch, Mohamed-Yassine, Vörös J, Abriel H, Ossola D, Amarouch MY, Zambelli T, Behr P   +18 more
core   +2 more sources

Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts

Bio-Protocol, 2021
The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and ...
Corey Landry, Mighten Yip, Ilya Kolb, William Stoy, Mercedes Gonzalez, Craig Forest   +5 more
doaj   +1 more source

Transfection methods for high-throughput cellular assays of voltage-gated calcium and sodium channels involved in pain.

PLoS ONE, 2021
Chemical transfection is broadly used to transiently transfect mammalian cells, although often associated with cellular stress and membrane instability, which imposes challenges for most cellular assays, including high-throughput (HT) assays.
Md Mahadhi Hasan, Lotten Ragnarsson, Fernanda C Cardoso, Richard J Lewis   +3 more
doaj   +1 more source