Results 31 to 40 of about 1,031,034 (270)
CFTR on an Automated Patch Clamp System [PDF]
Biophysical Journal, 2016 One of the significant challenges for Automated Patch Clamp (APC) instruments has been to measure leak ion channels such as Cystic Fibrosis Transmembrane conductance Regulator (CFTR). CFTR functions as a chloride channel and controls the regulation of different transport pathways.
Jacobsen, Rasmus B. +1 more
openaire +1 more source
The suitability of high throughput automated patch clamp for physiological applications [PDF]
The Journal of Physiology, 2021 AbstractAlthough automated patch clamp (APC) devices have been around for many years and have become an integral part of many aspects of drug discovery, high throughput instruments with gigaohm seal data quality are relatively new. Experiments where a large number of compounds are screened against ion channels are ideally suited to high throughput APC,
Alison Obergrussberger +14 more
openaire +4 more sources
Pharmaceuticals, 2022 Excessive Ca2+ currents via N-methyl-D-aspartate receptors (NMDARs) have been implicated in many disorders. Uncompetitive NMDAR channel blockers are an emerging class of drugs in clinical use for major depressive disorder (MDD) and other neuropsychiatric
Ezio Bettini +17 more
doaj +1 more source
Nanoscale-targeted patch-clamp recordings of functional presynaptic ion channels [PDF]
, 2013 Direct electrical access to presynaptic ion channels has hitherto been limited to large specialized terminals such as the calyx of Held or hippocampal mossy fiber bouton.
Rusakov, Dmitri A. +26 more
core +1 more source
Deep learning-based real-time detection of neurons in brain slices for in vitro physiology
Scientific Reports, 2021 A common electrophysiology technique used in neuroscience is patch clamp: a method in which a glass pipette electrode facilitates single cell electrical recordings from neurons.
Mighten C. Yip +4 more
doaj +1 more source
Membranes, 2022 Transient Receptor Potential Melastatin 8 (TRPM8) from the melastatin TRP channel subfamily is a non-selective Ca2+-permeable ion channel with multimodal gating which can be activated by low temperatures and cooling compounds, such as menthol and icilin.
Farhad Jahanfar +3 more
doaj +1 more source
Patch-clamp measurement of ICRAC and ORAI channel activity. [PDF]
, 2014 Depletion of internal Ca(2+) stores activates store-operated Ca(2+) channels. The most prominent members of this class of channels are Ca(2+) release-activated Ca(2+) (CRAC) channels, which are present in a variety of cell types including immune cells ...
Holzmann, Christian +5 more
core +1 more source
High-Throughput Automated Patch Clamp Investigations on Ion Channels in Erythrocytes [PDF]
, 2021 Trotz ihrer morphologischen Einfachheit ist die Membran der roten Blutkörperchen (Erythrozyten) mit einer Reihe von Transportern und Ionenkanälen ausgestattet, die bisher nicht vollständig charakterisiert sind und deren biologische Rolle noch wenig ...
Rotordam, Maria Giustina
core +1 more source
Setting up a new gold standard: automated temperature-controlled hERG test on Patchliner®
Frontiers in Pharmacology, 2012 In the present study, the Patchliner® temperature-controlled automated patch clamp system was evaluated for testing actions of drugs on K+ currents through hERG channels expressed in CHO cells at 35-37°C.
Liudmila ePolonchuk
doaj +1 more source
An efficient and scalable data analysis solution for automated electrophysiology platforms
SLAS Discovery, 2022 Ion channels are drug targets for neurologic, cardiac, and immunologic diseases. Many disease-associated mutations and drugs modulate voltage-gated ion channel activation and inactivation, suggesting that characterizing state-dependent effects of test ...
Tianbo Li +6 more
doaj +1 more source