Results 41 to 50 of about 1,044,066 (265)
Deep learning-based real-time detection of neurons in brain slices for in vitro physiology
Scientific Reports, 2021 A common electrophysiology technique used in neuroscience is patch clamp: a method in which a glass pipette electrode facilitates single cell electrical recordings from neurons.
Mighten C. Yip +4 more
doaj +1 more source
Characterization of iPS Derived Cardiomyocytes in Voltage Clamp and Current Clamp by Automated Patch Clamp [PDF]
Biophysical Journal, 2017 In recent years, human stem cell-derived cardiomyocytes have proven to recapitulate key features of human cardiac electrophysiology in vitro. Furthermore, it has become apparent that the intact ensemble of cardiac ion channels is necessary to determine proarrhythmic effects reliably.
Andrea Brüggemann +6 more
openaire +6 more sources
Membranes, 2022 Transient Receptor Potential Melastatin 8 (TRPM8) from the melastatin TRP channel subfamily is a non-selective Ca2+-permeable ion channel with multimodal gating which can be activated by low temperatures and cooling compounds, such as menthol and icilin.
Farhad Jahanfar +3 more
doaj +1 more source
Patch-clamp measurement of ICRAC and ORAI channel activity. [PDF]
, 2014 Depletion of internal Ca(2+) stores activates store-operated Ca(2+) channels. The most prominent members of this class of channels are Ca(2+) release-activated Ca(2+) (CRAC) channels, which are present in a variety of cell types including immune cells ...
Holzmann, Christian +5 more
core +1 more source
High-Throughput Automated Patch Clamp Investigations on Ion Channels in Erythrocytes [PDF]
, 2021 Trotz ihrer morphologischen Einfachheit ist die Membran der roten Blutkörperchen (Erythrozyten) mit einer Reihe von Transportern und Ionenkanälen ausgestattet, die bisher nicht vollständig charakterisiert sind und deren biologische Rolle noch wenig ...
Rotordam, Maria Giustina
core +1 more source
Setting up a new gold standard: automated temperature-controlled hERG test on Patchliner®
Frontiers in Pharmacology, 2012 In the present study, the Patchliner® temperature-controlled automated patch clamp system was evaluated for testing actions of drugs on K+ currents through hERG channels expressed in CHO cells at 35-37°C.
Liudmila ePolonchuk
doaj +1 more source
An efficient and scalable data analysis solution for automated electrophysiology platforms
SLAS Discovery, 2022 Ion channels are drug targets for neurologic, cardiac, and immunologic diseases. Many disease-associated mutations and drugs modulate voltage-gated ion channel activation and inactivation, suggesting that characterizing state-dependent effects of test ...
Tianbo Li +6 more
doaj +1 more source
, 2021 Standard high throughput screening projects using automated patch-clamp instruments often fail to grasp essential details of the mechanism of action, such as binding/unbinding dynamics and modulation of gating.
Mátyás C. Földi (11492557) +6 more
core +1 more source
Automated Electrophysiology Makes the Pace for Cardiac Ion Channel Safety Screening
Frontiers in Pharmacology, 2011 The field of automated patch-clamp electrophysiology has emerged from the tension between the pharmaceutical industry’s need for high-throughput compound screening versus its need to be conservative due to regulatory requirements.
Clemens eMoeller, Harry eWitchel
doaj +1 more source
Automated patch clamp supports functional
Blood Red Cells & Iron: Hereditary xerocytosis (HX) is a dominantly inherited hemolytic anemia caused by gain-of-function mutations in the mechanosensitive ion channel PIEZO1.
Kian Haseli +6 more
doaj +1 more source