Results 31 to 40 of about 1,044,066 (265)
Development of Automated Patch Clamp Technique to Investigate CFTR Chloride Channel Function. [PDF]
Front Pharmacol, 2017 International audienceThe chloride (Cl-) channel cystic fibrosis transmembrane conductance regulator (CFTR) is defective in cystic fibrosis (CF), and mutation of its encoding gene leads to various defects such as retention of the misfolded protein in the
Billet A, Froux L, Hanrahan JW, Becq F.
europepmc +2 more sources
Neuron Contact Detection Based on Pipette Precise Positioning for Robotic Brain-Slice Patch Clamps
Sensors, 2023 A patch clamp is the “gold standard” method for studying ion-channel biophysics and pharmacology. Due to the complexity of the operation and the heavy reliance on experimenter experience, more and more researchers are focusing on patch-clamp automation ...
Ke Li +6 more
doaj +1 more source
Wellcome Open Research, 2021 Automated patch-clamp platforms are widely used and vital tools in both academia and industry to enable high-throughput studies such as drug screening. A leak current to ground occurs whenever the seal between a pipette and cell (or internal solution and
Chon Lok Lei +7 more
doaj +1 more source
Automatic deep learning-driven label-free image-guided patch clamp system
Nature Communications, 2021 Patch clamp recording of neurons is slow and labor-intensive. Here the authors present a method for automated deep learning driven label-free image guided patch clamp physiology to perform measurements on hundreds of human and rodent neurons.
Krisztian Koos +13 more
doaj +1 more source
Force-controlled patch clamp of beating cardiac cells. [PDF]
, 2015 From its invention in the 1970s, the patch clamp technique is the gold standard in electrophysiology research and drug screening because it is the only tool enabling accurate investigation of voltage-gated ion channels, which are responsible for action ...
Mohamed-Yassine Amarouch (1640302) +18 more
core +2 more sources
Bio-Protocol, 2021 The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and ...
Corey Landry +5 more
doaj +1 more source
PLoS ONE, 2021 Chemical transfection is broadly used to transiently transfect mammalian cells, although often associated with cellular stress and membrane instability, which imposes challenges for most cellular assays, including high-throughput (HT) assays.
Md Mahadhi Hasan +3 more
doaj +1 more source
Pharmaceuticals, 2022 Excessive Ca2+ currents via N-methyl-D-aspartate receptors (NMDARs) have been implicated in many disorders. Uncompetitive NMDAR channel blockers are an emerging class of drugs in clinical use for major depressive disorder (MDD) and other neuropsychiatric
Ezio Bettini +17 more
doaj +1 more source
CFTR on an Automated Patch Clamp System [PDF]
Biophysical Journal, 2016 One of the significant challenges for Automated Patch Clamp (APC) instruments has been to measure leak ion channels such as Cystic Fibrosis Transmembrane conductance Regulator (CFTR). CFTR functions as a chloride channel and controls the regulation of different transport pathways.
Jacobsen, Rasmus B. +1 more
openaire +1 more source
Nanoscale-targeted patch-clamp recordings of functional presynaptic ion channels [PDF]
, 2013 Direct electrical access to presynaptic ion channels has hitherto been limited to large specialized terminals such as the calyx of Held or hippocampal mossy fiber bouton.
Rusakov, Dmitri A. +26 more
core +1 more source