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Rat Pheochromocytoma PC12 Cells in Culture
2022PC12 cells serve as a secretory cell model, especially suitable for studying the molecular mechanisms underlying fusion pore kinetics in regulated exocytosis of dense-core vesicles (DCVs). In this chapter, we describe a series of PC12 cell culture procedures optimized for real-time functional assays such as single-vesicle amperometry.
Pin-Chun, Chen, Chih-Tien, Wang
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Uracil salvage pathway in PC12 cells
Biochimica et Biophysica Acta (BBA) - General Subjects, 2000The salvage anabolism of uracil to pyrimidine ribonucleosides and ribonucleotides was investigated in PC12 cells. Pyrimidine base phosphoribosyl transferase is absent in PC12 cells. As a consequence any uracil or cytosine salvage must be a 5-phosphoribosyl 1-pyrophosphate-independent process.
MASCIA L. +3 more
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Paraquat-Induced Cell Death in PC12 Cells
Neurochemical Research, 1998Paraquat was taken up by PC12 cells in a carrier-mediated, saturable manner. When PC12 cells were permeabilized with digitonin (50 microg/ml) lipid peroxidation was observed after paraquat treatment in the presence of NADPH and chelated iron. The fact that lipid peroxidation preceded the appearance of LDH release provides positive evidence that lipid ...
W L, Yang, A Y, Sun
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Delivery of antisense oligonucleotides to PC12 cells
Neuroscience Research, 2002Optimal experimental conditions for the delivery of phosphodiester or phosphorothioate antisense oligonucleotides (P-ASO/S-ASO) to PC12 cells were determined. Fluorescently labeled P-ASO or S-ASO were transfected to PC12 cells and the uptake of antisense, free or entrapped in liposomes, was monitored by confocal and fluorescent microscopy.
Rosalinda, Acosta +3 more
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The Action of Adenosine Analogs on PC12 Cells
Journal of Neurochemistry, 1981Abstract: PC12 cells, a nerve growth factor–responsive clone of rat pheochromocytoma, contain a membrane–bound adenylate cyclase, which can be activated by adenosine analogs. The characteristics of the cyclase response indicate the presence of stimulatory adenosine receptors.
G, Guroff, G, Dickens, D, End, C, Londos
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Somatostatin enhances neurite outgrowth in PC12 cells
Developmental Brain Research, 1998The rat pheochromocytoma cell line PC12 forms neurites in response to nerve growth factor (NGF), and it was also reported to extend processes in the presence of somatostatin (somatotropin release-inhibiting factor, SRIF), a neuroactive peptide that seems to act as a morphogenetic factor in the developing nervous system.
TRAINA, Giovanna +3 more
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Manganese-induced apoptosis in PC12 cells
Neurotoxicology and Teratology, 2002Manganese has been known to induce neurological disorders similar to parkinsonisms for a long time. Dopamine deficiency has been demonstrated in Parkinson's disease and in chronic manganese poisoning, suggesting that the mechanisms underlying the neurotoxic effects of the metal ion are related to dysfunction of the extrapyramidal system.
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Clustering-Induced Signaling of CEACAM1 in PC12 Cells
Biological Chemistry, 2002Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), an Ig-like transmembrane protein, functions in cell adhesion, angiogenesis and epithelial cell morphogenesis, and has been identified as a tumor suppressor. For all of these functions, CEACAM1 requires signaling capabilities.
Matthias, Budt +3 more
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Cytotoxic effects of glutamic acid on PC12 cells☆
Neurochemistry International, 1994In order to investigate the biochemical mechanisms responsible for glutamate-induced cell death, we have tested the effect of this excitatory amino acid on the growth and survival of several cell lines of neural origin. Most of the cell lines studied were insensitive to glutamate, but we observed in PC12 cells that addition of glutamate (1-10 mmol/l ...
P, Froissard, D, Duval
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A Biochemical Assay for Acetylcholinesterase Activity in PC12 Cells
Science's STKE, 2007This lab describes two biochemical assays: One for measuring acetylcholinesterase activity and one for measuring protein concentration. Students learn how to manipulate small-volume samples, use a standard spectrophotometer or a microplate reader spectrophotometer, construct a standard curve, and normalize data.
Paul J, Schwartz +2 more
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