Results 131 to 140 of about 6,961 (161)

How sensitive is PCR-SSCP?

Human Mutation, 1993
Single-strand conformation polymorphism analysis (SSCP) is a rapid method for detection of minor sequence changes in polymerase chain reaction-amplified DNA. Since the first reported use of SSCP in 1989 (Orita et al., 1989), this technique has been used widely to detect mutations in oncogenes, tumor suppressor genes, and genes responsible for genetic ...
K, Hayashi, D W, Yandell
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Extensive mtDNA diversity in horses revealed by PCR–SSCP analysis

Animal Genetics, 1995
SummaryThe hypervariable D‐loop region of mitochondrial DNA (mtDNA) was amplified with the polymerase chain reaction using total horse DNA samples. Analysis of single strand conformation polymorphism (SSCP) of denatured amplification products was carried out by native polyacrylamide (8%) gel electrophoresis followed by silver staining.
S, Marklund, R, Chaudhary, L, Marklund, K, Sandberg, L, Andersson   +4 more
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Microhaplotype identified and performed in genetic investigation using PCR-SSCP

Forensic Science International: Genetics, 2017
The recently introduced concept of microhaplotype loci has attracted attention in forensics. Previous studies estimated the allele frequencies generally through obtaining genotypic data on the individual SNPs from a larger set of unrelated individuals then phasing microhaplotypes by statistical and computational techniques.
Peng, Chen, Jing, Zhu, Yan, Pu, Youjing, Jiang, Dan, Chen, Hui, Wang, Jiong, Mao, Bin, Zhou, Linbo, Gao, Peng, Bai, Weibo, Liang, Lin, Zhang   +11 more
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Application of PCR-SSCP Technology in Microorganism Detection

Advanced Materials Research, 2014
PCR-SSCP is a technology based on the combination of PCR and SSCP, widely used in such research fields as microorganism identification and microorganism flora. In this paper, the application of PCR-SSCP in bacterial detection and microbial community monitoring is briefly introduced.
Li Hong Bian, Ya Jun Lang
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PCR-SSCP: A method for detection of mutations

Genetic Analysis: Biomolecular Engineering, 1992
PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) analysis is one of the simplest and perhaps one of the most sensitive methods for detection of mutations based on PCR technology. The principles of PCR-SSCP, guidelines for experiments, and applications of this technique in various fields are reviewed.
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Allelic Variation in the Porcine MYF5 Gene Detected by PCR–SSCP

Molecular Biotechnology, 2008
The MYF5 gene has been reported to be integral to muscle growth and development, and hence it has been considered as a candidate gene for meat selection programs in pig. To ascertain whether there was variation in the porcine MYF5 gene, we have developed a method of PCR-single-strand conformational polymorphism (PCR-SSCP) analysis.
Sajee, Kunhareang, Huitong, Zhou, Jon G H, Hickford   +2 more
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[AP-PCR and AP-PCR-SSCP analyses].

Nihon rinsho. Japanese journal of clinical medicine, 1996
DNA fingerprinting by arbitrarily primed polymerase chain reaction (AP-PCR) is a simple and informative method to investigate genetic changes in cancers. The discovery of a mutator phenotype in hereditary non-polypotic colon cancer by the AP-PCR fingerprinting demonstrated the usefulness of this unbiased method.
J, Yasuda, A, Okano, T, Sekiya
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Distribution of MN genotypes detected by PCR-SSCP analysis

International Congress Series, 2003
Abstract Genotyping of the MN blood system was performed by means of PCR-single strand conformation polymorphism (PCR-SSCP) analysis. Twelve band patterns corresponding to each MN genotype composed of alleles MG, MT, N1, N2 and NV were detected. In general, MG or N1>MT>N2 in order of decreasing frequency was observed as four common alleles in five ...
N. Nakayashiki, J. Kanetake, Y. Sasaki, I. Yuasa, A. Miyoshi, M. Hashiyada, Y. Aoki   +6 more
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Polymorphism in the human E-selectin gene detected by PCR-SSCP

Human Genetics, 1994
By using the non-isotopic single-strand conformation polymorphism (SSCP) technique to analyse products of the polymerase chain reaction (PCR), we detected a 561-adenine to cytosine substitution resulting in an amino acid exchange from serine to arginine at position 128 of the E-selectin gene.
K, Wenzel, R, Hanke, A, Speer
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No TP53 mutations in neuroblastomas detected by PCR‐SSCP analysis

Genes, Chromosomes and Cancer, 1994
AbstractWe have analysed 29 neuroblastomas for TP53 mutations in exons 5 to 8 by means of the polymerase chain reaction in combination with the single‐strand conformation polymorphism technique. We could not detect any mutation. These results indicate that, in contrast to the majority of tumors so far studied, TP53 mutations do not seem to be important
J S, Castresana, M J, Bello, J A, Rey, P, Nebreda, A, Queizán, P, García-Miguel, A, Pestaña   +6 more
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