Results 141 to 150 of about 6,961 (161)
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The application of asymmetric PCR-SSCP in gene mutation detecting
Frontiers of Agriculture in China, 2008The advantages and disadvantages of asymmetric PCR-SSCP and the traditional PCR-SSCP were compared in this study. The mutations in 3′UTR of Smad4 gene of Luxi cattle and the Holstein cow were analyzed by asymmetric PCR-SSCP and one insert “T” mutation and one G/A mutation in this region were found.
Xiaohui Zhang +5 more
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Efficient detection of alport syndromeCOL4a5 mutations with multiplex genomic PCR-SSCP
American Journal of Medical Genetics, 2001We have performed effective mutation screening of COL4A5 with a new method of direct, multiplex genomic amplification that employs a single buffer condition and PCR profile. Application of the method to a consecutive series of 46 United States patients with diverse indications of Alport syndrome resulted in detection of mutations in 31 cases and of ...
D F, Barker +3 more
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[HLA-DQB2 genotypes analyzed by PCR/SSCP method].
Nihon hoigaku zasshi = The Japanese journal of legal medicine, 1996When the PCR products amplified by the primers prepared at the 11th HLA Workshop (DQBAMP-A, DQBAMP-B) were analyzed directly by the SSCP method, one or two pairs of characteristic bands were detected other than those attributed to DQB1, and a total of three kind of paired bands were detected.
K, Akiyama +3 more
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Mutation Screening Using PCR-SSCP: Silver Staining and Isotopic Protocols
2003Screening for mutations prior to sequencing can reduce the time and costs of identifying mutations. When the DNA sequence is known, the technique of detecting mutations as single-stranded conformational polymorphisms (SSCP) is a convenient method of screening for possible mutations. SSCP was originally developed by Orita et al. (1).
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DLA‐DRB1 and DLA‐DQB1 histocompatibility typing by PCR‐SSCP and sequencing
Tissue Antigens, 1998Abstract: The dog has been an important model for solid organ and hematopoietic stem cell transplantation for over 30 years. Fundamental to the continuing usage of the model is the development of molecular‐based histocompatibility typing of donors and recipients.
J L, Wagner, J D, Works, R, Storb
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[PCR SSCP study of an echovirus 30 meningitis outbreak].
Pathologie-biologie, 1999Between April and October 1997, 21 children of 4 days to 13 years old were admitted to the Pedatric Unit of Aulnay Sous Bois's Hospital for viral meningitidis. The number of white blood cells in the cerebrospinal fluid (CSF) was between 1 and 612 cells/mm3, with, on an average, 56% of segmented cells, 34% lymphocytes and 34% monocytes.
L, Maisonneuve +6 more
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Analiza mutacija u ALG6 genu PCR-SSCP metodom
2006THE ANALYSIS OF THE MUTATIONS IN ALG6 GENE BY PCR-SSCP METHOD S. Šupraha and J. Dumić Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, Zagreb, Croatia ; e-mail: jdumic@pharma.hr ; sandras@pharma.hr Congenital disorders of glycosylation (CDGs) are inherited autosomal recessive ...
Šupraha, Sandra, Dumić Jerka
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Detection of Mutations in Mycobacteria by PCR-SSCP (Single-Strand Conformation Polymorphism)
2003Keywords: Mutation ; Polymorphism ; Single-Stranded Conformational Reference EPFL-CHAPTER-151281doi:10.1385/0-89603-471-2:423View record in PubMed Record created on 2010-09-07, modified on 2017-05 ...
A, Telenti, N, Honoré, S T, Cole
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PCR‐SSCP in the ovine calpastatin gene
Animal Genetics, 1996N, Roberts +3 more
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Genotipizacija CYP2D6 *3 i *4 mutacija metodom PCR-SSCP
1999Single-Strand Conformation Polymorphism (SSCP) je metoda detekcije poznatih i nepoznatih mutacija koja se temelji na promjeni sekundarne strukture definiranog fragmenta jednolančane DNA. Cilj ovog rada je bio optimirati PCR-SSCP metodu detekcije CYP2D6 *3 and *4 mutacija korištenjem GMA gelova u Elchrom Scientific SEA 2000 elektroforetskom sustavu ...
Ivanišević, Ana-Maria +2 more
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