Results 71 to 80 of about 11,551 (206)

结核分支杆菌链霉素耐药的快速检测

Zhongshan Daxue xuebao. Yixue kexue ban, 2002
【目的】探讨用聚合酶链反应-单链构象多态性( PCR-SSCP)方法快速诊断结核分支杆菌链霉素( Sm)耐药的临床意义。【方法】用 PCR-SSCP 技术检测了 30 株Sm 敏感的结核分支杆菌临床分离株, 30 株 Sm 耐药的临床分离株,以结核分支杆菌 H37RV 标准株作对照。先用 PCR 方法扩增 rpsL 基因,随后用SSCP 方法鉴定其扩增产物有无突变。【结果】所有临床分离株均观察到 rpsL 基因 PCR 扩增产物。30 株 Sm 敏感的临床分离株与 H37RV 标准株呈现一样 SSCP ...
吴为群, 谢灿茂
doaj  

DFNB59 Gene Mutation Screening Using PCR-SSCP/HA Technique in Non-syndromic Genetic Hearing Loss in Bushehr Province [PDF]

, 2010
Background: Hearing impairment (HI) is the most prevalent Neurosensory disorder which is heterogenous and can also occur due to environmental causes. The majority of hearing deficiencies are of genetic origin affecting about 60% of the HI cases.
Asgari, Azam., Azadegan-Dehkordi, Fatemeh., Banitalebi, Golandam., Banitalebi, Mehdi., Farrokhi, Effat., Hashemzadeh-Chaleshtori, Morteza., kasiri, mahboobeh., Mobini, Gholam Reza., Montazer Zohouri, Mostafa., Raiesi, Marzieh., Raiesi, Somayeh., safari, javad., Shirmardi, Seyed Abolfath., Soleimani, Mahshid., Taherzadeh Ghahfarrokhi, Maryam., Taji, Fatemeh.   +15 more
core  

Historical fragmentation and stepping‐stone gene flow led to population genetic differentiation in a coastal seabird

Ecology and Evolution, Volume 14, Issue 4, April 2024.
The relative influence of the microevolutionary influencing population genetic structure in highly mobile organisms is poorly understood. Here we analyze genetic variation in a coastal seabird from the northeast Pacific Ocean. We find that, unlike most high latitude, Northern Hemisphere seabirds, pigeon guillemots display significant population genetic
Bronwyn A. S. Harkness, Gabriela Ibarguchi, Veronica F. Poland, Vicki L. Friesen   +3 more
wiley   +1 more source

Single Strand Conformation Polymorphism analysis of PCR-amplified rDNA to differentiate medically important Aspergillus Species [PDF]

Iranian Journal of Public Health, 2008
"nBackground: Aspergillus species are associated with allergic bronchopulmonary disease, mycotic keratitis, otomycosis, na­sal sinusitis and invasive infection.
K Diba, SH Mirhendi, P Kordbacheh, N Jalalizand   +3 more
doaj   +2 more sources

Molecular characterization of Iranian patients with possible familial hypercholesterolemia. [PDF]

, 2011
Familial hypercholesterolemia (FH) is an autosomal dominant disorder of lipoprotein metabolism caused mainly by mutations in the low-density lipoprotein receptor (LDLR) and apolipoprotein B 100 (APOB) genes.
Asadi Mobarakeh, S., Farrokhi, Effat., Ghatreh Samani, Keihan., Hashemzadeh-Chaleshtori, Morteza., Roghani Dehkordi, F., Shayesteh, Fatemeh.   +5 more
core   +1 more source

[The application of PCR-SSCP in forensic mtDNA typing].

Fa yi xue za zhi, 2010
To study the application of PCR-SSCP in forensic mtDNA typing.Primers flanking the mtDNA HV-I and HV-II regions were designed. By PCR-SSCP techniques, 70 family trios and 140 unrelated Wuhan Han individuals were investigated and analyzed.In 70 family trios, the SSCP profiles in region HV-I and HV-II of children were not same to that of their fathers in
Hong-Wei, Ai, Chun-Ying, Yu, Gui-Sen, Zhao, Dai-Xin, Huang, Ji, Ke, Xia, Yue, Qing-En, Yang   +6 more
openaire   +2 more sources

Genetic variants in the TAC1 transcriptional regulatory region affect on trainability and excitability levels in Belgian Malinois dogs

Veterinary Medicine and Science, Volume 10, Issue 1, January 2024.
In the present study, we investigate three candidate genes for behavioural traits in the Belgian Malinois, aiming to identify genetic variation that influences behavioural traits, for example, trainability. Our finding suggests that the TAC1 gene variants could influence transcription factor binding and, subsequently, the trainability and excitability ...
Mohammad Fallahi, Ali Akbar Masoudi, Rasoul Vaez Torshizi   +2 more
wiley   +1 more source

Single strand conformation polymorphism (SSCP) detection in six genes in the Portuguese indigenous sheep breed "Churra da Terra Quente" [PDF]

, 2012
Evaluation of the genetic diversity for six genes in forty animals of the Portuguese indigenous sheep breed (Ovis aries) “Churra da Terra Quente” was done.
Azevedo, Jorge, Bastos, Estela, Cravador, A., Guedes-Pinto, Henrique   +3 more
core  

THE LACK OF CORRELATION BETWEEN TP53 MUTATIONS AND GASTRIC CANCER: A REPORT FROM A PROVINCE OF IRAN [PDF]

, 2017
Gastric cancer ranks second cause of cancer death worldwide after lung cancer. Its etiology is heterogeneous and genetic factors including protooncogenes and tumor suppressor genes always contribute to the progression of cancer. The TP53 tumor suppressor
Farokhi, Effat., Ghasemi-Dehkordi, Payam., Hashemzadeh-Chaleshtori, Morteza., Moradi, Mohammad-Taghi., Saffari-Chaleshtori, Javad., Tabatabaiefar, Mohammad Amin.   +5 more
core   +1 more source

Species Identification of Totoaba (Totoaba macdonaldi) From Swim Bladders by Real‐Time Fluorescence‐Based Recombinase‐Aided Amplification

Journal of Marine Sciences, Volume 2024, Issue 1, 2024.
The increased illegal fishing to supply swim bladders is the current threat to the Totoaba macdonaldi, due to the high commercial value of swim bladders. Species identification is the foundation of the endangered animal protection. Limited by the sophisticated thermal cycling, specialized machine, and sequencing costs, DNA barcoding might not better ...
Hao Xu, Songqi Zhu, Yun Huang, Yan Deng, Hong Liu, Xiaocong Zheng, Haoyun Tang   +6 more
wiley   +1 more source