Results 121 to 130 of about 4,982,154 (391)

Emerging role of ARHGAP29 in melanoma cell phenotype switching

Molecular Oncology, EarlyView.
This study gives first insights into the role of ARHGAP29 in malignant melanoma. ARHGAP29 was revealed to be connected to tumor cell plasticity, promoting a mesenchymal‐like, invasive phenotype and driving tumor progression. Further, it modulates cell spreading by influencing RhoA/ROCK signaling and affects SMAD2 activity. Rho GTPase‐activating protein
Beatrice Charlotte Tröster, Melanie Kappelmann‐Fenzl, Anja Katrin Bosserhoff, Nicole Rachinger   +3 more
wiley   +1 more source

Examining peak height ratios in low template DNA samples with and without sampling using a single-tube extraction protocol [PDF]

, 2015
The developments of the polymerase chain reaction (PCR) and the short tandem repeat multiplex kits increased the ease and lowered the time and sample quantity required for deoxyribonucleic acid (DNA) typing compared to previous methods.
Nguyen, Thutrang Thi
core   +1 more source

Service evaluation to establish the sensitivity, specificity and additional value of broad-range 16S rDNA PCR for the diagnosis of infective endocarditis from resected endocardial material in patients from eight UK and Ireland hospitals [PDF]

, 2014
Infective endocarditis (IE) can be diagnosed in the clinical microbiology laboratory by culturing explanted heart valve material. We present a service evaluation that examines the sensitivity and specificity of a broad-range 16S rDNA polymerase chain ...
Alshafi, K, Gouliouris, T, Harris, KA, Hartley, JC, Jalili, S, Munthali, P, NiRiain, U, Williams, OM, Yam, T   +8 more
core   +1 more source

[Reverse transcriptase PCR (RT-PCR) and quantitative-competitive PCR (QC-PCR)].

Experimental & molecular medicine, 2002
Due to its extraordinarily high sensitivity, polymerase chain reaction (PCR) is widely used for amplifying cDNA copies of low abundance mRNA. However, quantitation is unreliable because the amount of PCR product increases exponentially with each cycle of amplification; therefore, minute differences in any of the variables that affect the efficiency of ...
openaire   +1 more source

Gut microbiota diversity is prognostic in metastatic hormone receptor‐positive breast cancer patients receiving chemotherapy and immunotherapy

Molecular Oncology, EarlyView.
In this exploratory study, we investigated the relationship between the gut microbiota and outcome in patients with metastatic hormone receptor‐positive breast cancer, treated in a randomized clinical trial with chemotherapy alone or chemotherapy in combination with immune checkpoint blockade.
Andreas Ullern, Kristian Holm, Nikolai Kragøe Andresen, Andreas Hagen Røssevold, Corinna Bang, Bjørn Naume, Johannes R. Hov, Jon Amund Kyte   +7 more
wiley   +1 more source

Performance of chest X-ray with computer-aided detection powered by deep learning-based artificial intelligence for tuberculosis presumptive identification during case finding in the Philippines

BMC Global and Public Health
Background The Philippines’ high tuberculosis (TB) burden calls for effective point-of-care screening. Systematic TB case finding using chest X-ray (CXR) with computer-aided detection powered by deep learning-based artificial intelligence (AI-CAD ...
Nichel Marquez, Erwin John Carpio, Mary Rosary Santiago, Jeremiah Calderon, Ruth Orillaza-Chi, Shawnee Shaine Salanap, Lisa Stevens   +6 more
doaj   +1 more source

Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification

Scientific Reports, 2018
Single cell genome analysis methods are powerful tools to define features of single cells and to identify differences between them. Since the DNA amount of a single cell is very limited, cellular DNA usually needs to be amplified by whole-genome ...
Christiane Bäumer, Evelyn Fisch, Holger Wedler, Frank Reinecke, Christian Korfhage   +4 more
doaj   +1 more source

Selection and validation of reference genes for gene expression studies in Pseudomonas brassicacearum GS20 using real-time quantitative reverse transcription PCR [PDF]

, 2020
Bianxia Bai, Jiahong Ren, Fenling Bai, Hao Lin   +3 more
openalex   +1 more source

High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number

Analytical Chemistry, 2011
Digital PCR enables the absolute quantitation of nucleic acids in a sample. The lack of scalable and practical technologies for digital PCR implementation has hampered the widespread adoption of this inherently powerful technique. Here we describe a high-
B. Hindson, K. Ness, D. Masquelier, P. Belgrader, Nick Heredia, A. Makarewicz, Isaac J. Bright, M. Lucero, A. Hiddessen, T. Legler, Tyler K. Kitano, Michael R. Hodel, J. Petersen, Paul W Wyatt, Erin R. Steenblock, Pallavi H. Shah, L. Bousse, C. Troup, Jeff Mellen, Dean K. Wittmann, Nicholas G. Erndt, T. Cauley, R. Koehler, A. So, S. Dube, K. Rose, Luz Montesclaros, Shenglong Wang, D. Stumbo, Shawn Hodges, S. Romine, F. Milanovich, H. White, John F. Regan, G. Karlin-Neumann, Christopher M. Hindson, Serge Saxonov, B. Colston   +37 more
semanticscholar   +1 more source

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source