Results 291 to 300 of about 3,342,563 (345)

Colony PCR

2013
Colony PCR is a method for rapidly screening colonies of yeast or bacteria that have grown up on selective media following a transformation step, to verify that the desired genetic construct is present, or to amplify a portion of the construct.
Bergkessel, Megan, Guthrie, Christine
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Quantitative PCR

cclm, 2000
Abstract The classic molecular biology methods like Northern or Southern blot analyse non-amplified DNA or RNA, but need large amounts of nucleic acids, in many instances from tissues or cells that are heterogeneous. In contrast, polymerase chain reaction (PCR)-based techniques allow us to obtain genetic information through the specific ...
R, Jung, K, Soondrum, M, Neumaier
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Microchip PCR

Analytical and Bioanalytical Chemistry, 2003
Miniaturization of genetic tests has become an important goal. This review surveys the current progress towards the miniaturization of tests based on the polymerase chain reaction (PCR). It examines the different types of PCR microchip designs, fabrication methods,and the components of a microchip PCR device. It also discusses the problems attributable
L J, Kricka, P, Wilding
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Reverse-Transcription PCR (RT-PCR)

2013
RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also commonly used to clone cDNAs for further use with other molecular biology techniques (e.g.,
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