Results 41 to 50 of about 3,284,156 (346)

Positive RT-PCR Test Results in Patients Recovered From COVID-19.

Journal of the American Medical Association (JAMA), 2020
This case series describes reverse transcriptase–polymerase chain reaction (RT-PCR) test results in 4 health professionals discharged from hospitalization or quarantine after 2 negative RT-PCR test results and resolution of clinical COVID-19 infection.
Lan Lan, Dan Xu, Guangming Ye, Chen Xia, Shaokang Wang, Yirong Li, Haibo Xu   +6 more
semanticscholar   +1 more source

Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation

International Journal of Analytical Chemistry, 2015
A semiautomated extraction protocol of HCV-RNA using Favorgen RNA extraction kit has been developed. The kit provided protocol was modified by replacing manual spin steps with vacuum filtration. The assay performance was evaluated by real-time qPCR based
Imran Amin, Tania Jabbar, Fawad Niazi, Muhammad Saeed Akhtar   +3 more
doaj   +1 more source

ERBIN limits epithelial cell plasticity via suppression of TGF‐β signaling

FEBS Letters, EarlyView.
In breast and lung cancer patients, low ERBIN expression correlates with poor clinical outcomes. Here, we show that ERBIN inhibits TGF‐β‐induced epithelial‐to‐mesenchymal transition in NMuMG breast and A549 lung adenocarcinoma cell lines. ERBIN suppresses TGF‐β/SMAD signaling and reduces TGF‐β‐induced ERK phosphorylation.
Chao Li, Gerard van der Zon, Peter ten Dijke, Tong Shen   +3 more
wiley   +1 more source

Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons.

Genome Research, 2011
Bacterial diversity among environmental samples is commonly assessed with PCR-amplified 16S rRNA gene (16S) sequences. Perceived diversity, however, can be influenced by sample preparation, primer selection, and formation of chimeric 16S amplification ...
B. Haas, D. Gevers, A. Earl, M. Feldgarden, D. Ward, G. Giannoukos, Dawn M. Ciulla, D. Tabbaa, S. Highlander, E. Sodergren, B. Methé, T. DeSantis, J. Petrosino, R. Knight, B. Birren   +14 more
semanticscholar   +1 more source

Real-time RT-PCR in COVID-19 detection: issues affecting the results

Expert Review of Molecular Diagnostics, 2020
Due to the rapid spread and increasing number of coronavirus disease 19 (COVID-19) cases caused by a new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), rapid and accurat...
A. Tahamtan, A. Ardebili
semanticscholar   +1 more source

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

FEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield, Torsten Kleffmann, David J. Saul, Sigurd M. Wilbanks   +3 more
wiley   +1 more source

Validation of a DIVA qPCR Duplex Assay to Differentiate Primun Salmonella T Vaccine from Salmonella enterica subsp. enterica Serovar Typhimurium Wild Strains

Applied Sciences
Salmonella enterica subsp. enterica serovar Typhimurium is an important foodborne pathogen, and poultry products are a major source of human infection.
Antonio Martínez-Murcia, Aaron Navarro, Adrián García-Sirera, Paloma Romero, Caridad Miró-Pina, Jesús María Rubio, Ante Blazevic   +6 more
doaj   +1 more source

PCR Detection of Mimivirus [PDF]

Emerging Infectious Diseases, 2017
In an epidemiological study, mimivirus was reported as an unlikely cause of human respiratory infections in China. Our analysis revealed the nonsensitivity of the PCR method, which detected less than 10% of the current known mimivirus. We conclude that epidemiologic studies must use accurate and sensitive laboratory test methods.
Raoult, Didier, Levasseur, Anthony, La Scola, Bernard   +2 more
openaire   +4 more sources

Stability issues of RT‐PCR testing of SARS‐CoV‐2 for hospitalized patients clinically diagnosed with COVID‐19

Journal of Medical Virology, 2020
In this study, we collected a total of 610 hospitalized patients from Wuhan between February 2, 2020, and February 17, 2020. We reported a potentially high false negative rate of real‐time reverse‐transcriptase polymerase chain reaction (RT‐PCR) testing ...
Yafang Li, L. Yao, Jiawei Li, Lei Chen, Yiyan Song, Z. Cai, Chunhua Yang   +6 more
semanticscholar   +1 more source

C‐mannosylation promotes ADAMTS1 activation and secretion in human testicular germ cell tumor NEC8 cells

FEBS Letters, EarlyView.
C‐mannosylation is a unique form of protein glycosylation. In this study, we demonstrated that ADAMTS1 is C‐mannosylated at Trp562 and Trp565 in human testicular germ cell tumor NEC8 cells. We found that C‐mannosylation of ADAMTS1 is essential for its secretion, processing, enzymatic activity, and ability to promote vasculogenic mimicry. These findings
Takato Kobayashi, Takehiro Suzuki, Ryota Kawahara, Natsumi Harai, Naoshi Dohmae, Siro Simizu   +5 more
wiley   +1 more source