Results 41 to 50 of about 3,342,563 (345)
Positive RT-PCR Test Results in Patients Recovered From COVID-19.
This case series describes reverse transcriptase–polymerase chain reaction (RT-PCR) test results in 4 health professionals discharged from hospitalization or quarantine after 2 negative RT-PCR test results and resolution of clinical COVID-19 infection.
Lan Lan +6 more
semanticscholar +1 more source
Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation
A semiautomated extraction protocol of HCV-RNA using Favorgen RNA extraction kit has been developed. The kit provided protocol was modified by replacing manual spin steps with vacuum filtration. The assay performance was evaluated by real-time qPCR based
Imran Amin +3 more
doaj +1 more source
An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
wiley +1 more source
Background: Human herpesvirus 8 (HHV-8) stimulates lymphoproliferation, by activating the signaling pathway of the interleukin-6 receptor, along with several other regulatory mechanisms.
Ameer J Abbas Al-Jawdhari +1 more
doaj +1 more source
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
Salmonella enterica subsp. enterica serovar Typhimurium is an important foodborne pathogen, and poultry products are a major source of human infection.
Antonio Martínez-Murcia +6 more
doaj +1 more source
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang +5 more
wiley +1 more source
Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang +8 more
wiley +1 more source
Penerapan Metode UCD (User Centered Design) Pada E-Commerce Putri Intan Shop Berbasis Web
Pertumbuhan pesat pangsa pasar e-commerce di Indonesia memang sudah tidak bisa diragukan lagi. Dengan jumlah pengguna internet yang mencapai angka 82 juta orang atau sekitar 30% dari total penduduk di Indonesia, pasar e-commerce menjadi tambang emas yang
Intan Sandra Yatana Saputri +2 more
doaj +1 more source
Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data
Despite the central role of quantitative PCR (qPCR) in the quantification of mRNA transcripts, most analyses of qPCR data are still delegated to the software that comes with the qPCR apparatus. This is especially true for the handling of the fluorescence
J. Ruijter +6 more
semanticscholar +1 more source

