Results 101 to 110 of about 109,234 (306)

Peptide Coacervates Promote Cytosolic Delivery of STING Agonists for Cancer Immunotherapy

Vaccines
Background/Objectives: Cyclic dinucleotide stimulator of interferon genes (STING) agonists have emerged as potential agents in cancer immunotherapy, but their clinical applications are limited by relatively poor pharmacokinetic properties.
Wenlv Zheng, Wei Tang, Jianzheng Wang, Yurong Li, Shengnan Wang, Dan Wu, Xiaoquan Wang, Junmin Quan   +7 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Insights into How Cyclic Peptides Switch Conformations

, 2016
Cyclic peptides have recently emerged as promising modulators of protein–protein interactions. However, it is currently highly difficult to predict the structures of cyclic peptides owing to their rugged conformational free energy landscape, which ...
Hongtao Yu (43676), Yu-Shan Lin (519689), Sean M. McHugh (2607055), Julia R. Rogers (2607052)   +3 more
core   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Crystallization, sequence and preliminary crystallographic data for transmission-blocking anti-malaria Fab 4B7 with cyclic peptides from the Pfs25 protein of P. falciparum

, 1994
X-ray quality crystals of an Fab fragment from a transmission-blocking monoclonal antibody 4B7 (MAb 4B7) against a sexual stage protein Pfs25 of Plasmodium falciparum were grown as uncomplexed and peptide-complexed forms.
Kang, A.S., Stura, E.A., Satterthwait, A.C., Stefanko, R.S., Calvo, J.C., Kaslow, D.C.   +5 more
core   +1 more source

The occurrence of ansamers in the synthesis of cyclic peptides

, 2022
α-Amanitin is a bicyclic octapeptide composed of a macrolactam with a tryptathionine cross-link forming a handle. Previously, the occurrence of isomers of amanitin, termed atropisomers has been postulated.
Roderich, Süssmuth, Marius, Wenz, Elisabeth, Irran, Bettina, Keller, Guiyang, Yao, Simone, Kosol, Oliver , Trapp   +6 more
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

The Calbindin-D28k binding site on inositol monophosphatase may allow inhibition independent of the lithium site of action

, 2011
Among numerous reported biochemical effects the lithium-inhibitable enzyme inositol-monophosphatase (IMPase) remains a viable target for lithium's therapeutic mechanism of action.
Galila Agam, Itshak Levi, Orna Almog, Miriam Eisenstein, Joseph Fanous, Yael Askira, Amnon Hoffman, Yftah Talgan, Chaim Gilon   +8 more
core  

Synthesis of cyclic peptides as bioconjugation platforms

, 2021
Cyclic peptides are investigated as a platform to induce different orientations between various ligands. Through click chemistry, a variety of Toll-like receptor ligands can be attached to the cyclic peptides, which are based on gramicidin S.Bio-organic ...
Peterse, E.
core