Results 161 to 170 of about 9,126 (180)

Measure of Peptidoglycan Hydrolase Activity

2017
Most gene clusters encoding multiprotein complexes of the bacterial cell envelope, such as conjugation and secretion systems, Type IV pili, and flagella, bear a gene encoding an enzyme with peptidoglycan hydrolase activity. These enzymes are usually glycoside hydrolases that cleave the glycan chains of the peptidoglycan.
Yoann G, Santin, Eric, Cascales
openaire   +2 more sources

Peptidoglycan Hydrolases of Bacillus subtilis 168

Microbial Drug Resistance, 1996
ABSTRACT There are multiple peptidoglycan hydrolases associated with Bacillus subtilis 168 and these potentially lethal enzymes have been implicated in a number of important cellular processes.
T J, Smith, S A, Blackman, S J, Foster
openaire   +2 more sources

Peptidoglycan hydrolases in an autolytic mutant of Salmonella typhimurium

Archives Internationales de Physiologie et de Biochimie, 1988
Two peptidoglycan hydrolases were isolated from the autolytic mutant Salmonella typhimurium DA361 (envD). One of them, resistant to penicillin, was found free in the supernatant of partially purified envelopes sedimented by ultracentrifugation, and the other bound to the envelopes proved to be sensitive to the antibiotic. Both were able to hydrolyse in
R A, Pizarro, R O, Fernández, L V, Orce
openaire   +2 more sources

From the regulation of peptidoglycan synthesis to bacterial growth and morphology

Nature Reviews Microbiology, 2011
How bacteria grow and divide while retaining a defined shape is a fundamental question in microbiology, but technological advances are now driving a new understanding of how the shape-maintaining bacterial peptidoglycan sacculus grows. In this Review, we
Athanasios Typas, Manuel Banzhaf, Carol A Gross   +2 more
exaly   +2 more sources

Biochemical analysis of NlpC/p60 peptidoglycan hydrolase activity

2020
The NlpC/p60-family of peptidoglycan hydrolases are key enzymes that facilitate bacterial cell division and also modulate microbe-host interactions. These endopeptidases utilize conserved Cys-His residues in their active site and are expressed in most bacterial species as well as some eukaryotes.
Byungchul, Kim, Juliel, Espinosa, Howard C, Hang   +2 more
openaire   +2 more sources

Identification of a novel peptidoglycan hydrolase CwlM in Mycobacterium tuberculosis

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2005
Mycobacterium tuberculosis is a major global pathogen whose threat has increased with the emergence of multidrug-resistant strains. The cell wall of M. tuberculosis is thick, rigid, and hydrophobic, which serves to protect the organism from the environment and makes it highly impermeable to conventional antimicrobial agents. There is little known about
Lingyi Lynn, Deng, Donald E, Humphries, Robert D, Arbeit, Laura E, Carlton, Sandra C, Smole, J David, Carroll   +5 more
openaire   +2 more sources

Discovery and Development of Antibacterial Peptidoglycan Hydrolase Inhibitors

ACS Infectious Diseases
The growing number of bacterial infections and the rise of antibiotic resistance require approaches for antimicrobial development. Peptidoglycan, essential for maintaining the integrity and shape of the bacterial cell wall, is regulated by the coordinated activity of peptidoglycan synthesis and remodeling enzymes.
Kyong Tkhe Fam, Pavan Kumar Chodisetti, Howard C. Hang   +2 more
openaire   +2 more sources

Structural Comparison and Simulation of Pneumococcal Peptidoglycan Hydrolase LytB

2016
Three-dimensional structural determination combined with comprehensive comparisons with the homologs is a straightforward strategy to decipher the molecular function of an enzyme. However, in many cases it's difficult to obtain the complex structure with the substrate/ligand.
Xiao-Hui, Bai, Qiong, Li, Yong-Liang, Jiang, Jing-Ren, Zhang, Yuxing, Chen, Cong-Zhao, Zhou   +5 more
openaire   +2 more sources

Characterization of peptidoglycan hydrolase in Cag pathogenicity island of Helicobacter pylori

Molecular Biology Reports, 2010
The Cag Type IV secretion apparatus proteins in Helicobacter pylori can mediate the injection of effector CagA protein into eukaryotic target cells. Although this apparatus forms an important pathway for bacterium-host interaction, its assembly process in vivo is poorly understood, and the proteins which contribute to break the bacterial cell walls in ...
Qiao, Zhong, Shihe, Shao, Runhong, Mu, Hua, Wang, Shiteng, Huang, Jun, Han, He, Huang, Shuwei, Tian   +7 more
openaire   +2 more sources

Crystal structure of the glycosidase family 73 peptidoglycan hydrolase FlgJ

Biochemical and Biophysical Research Communications, 2009
Glycoside hydrolase (GH) categorized into family 73 plays an important role in degrading bacterial cell wall peptidoglycan. The flagellar protein FlgJ contains N- and C-terminal domains responsible for flagellar rod assembly and peptidoglycan hydrolysis, respectively. A member of family GH-73, the C-terminal domain (SPH1045-C) of FlgJ from Sphingomonas
Wataru, Hashimoto, Akihito, Ochiai, Keiko, Momma, Takafumi, Itoh, Bunzo, Mikami, Yukie, Maruyama, Kousaku, Murata   +6 more
openaire   +2 more sources