Results 1 to 10 of about 6,966 (163)

Computational insights into the natural phage endolysin linker landscape [PDF]

BioData Mining
Phage endolysins are increasingly investigated as novel protein-based antibiotics, offering solutions to the antibiotic resistance crisis. Endolysins targeting Gram-positive bacteria come in a variety of modular architectures, combining domains that bind
Emma Cremelie, Alexandre Boulay, Roberto Vázquez, Yves Briers   +3 more
doaj   +3 more sources

Combination Treatment for Inhibition of the Growth of Staphylococcus aureus with Recombinant SAP8 Endolysin and Nisin

Antibiotics, 2022
Staphylococcus aureus, a pathogenic species of genus Staphylococcus involved in foodborne illness always remain among the top priorities of the world major concerns.
Seon-Gyu Kim, Shehzad Abid Khan, Young-Duck Lee, Jong-Hyun Park, Gi-Seong Moon   +4 more
doaj   +2 more sources

Biochemical Characterization and Validation of a Catalytic Site of a Highly Thermostable Ts2631 Endolysin from the Thermus scotoductus Phage vB_Tsc2631. [PDF]

PLoS ONE, 2015
Phage vB_Tsc2631 infects the extremophilic bacterium Thermus scotoductus MAT2631 and uses the Ts2631 endolysin for the release of its progeny. The Ts2631 endolysin is the first endolysin from thermophilic bacteriophage with an experimentally validated ...
Magdalena Plotka, Anna-Karina Kaczorowska, Agnieszka Morzywolek, Joanna Makowska, Lukasz P Kozlowski, Audur Thorisdottir, Sigurlaug Skírnisdottir, Sigridur Hjörleifsdottir, Olafur H Fridjonsson, Gudmundur O Hreggvidsson, Jakob K Kristjansson, Slawomir Dabrowski, Janusz M Bujnicki, Tadeusz Kaczorowski   +13 more
doaj   +2 more sources

Diversity of the lysozyme fold: structure of the catalytic domain from an unusual endolysin encoded by phage Enc34

Scientific Reports, 2022
Endolysins are bacteriophage-encoded peptidoglycan-degrading enzymes with potential applications for treatment of multidrug-resistant bacterial infections. Hafnia phage Enc34 encodes an unusual endolysin with an N-terminal enzymatically active domain and
Elina Cernooka, Janis Rumnieks, Nikita Zrelovs, Kaspars Tars, Andris Kazaks   +4 more
doaj   +2 more sources

Effect of different cloning strategies in pET-28a on solubility and functionality of a staphylococcal phage endolysin [PDF]

BioTechniques, 2020
Endolysins have been studied intensively as an alternative to antibiotics. In this study, endolysin derived from a phage which infects methicillin-resistant Staphylococcus aureus (MRSA) was cloned and expressed in Escherichia coli pET28a.
Hong Y Tham, Adelene A-L Song, Khatijah Yusoff, Geok H Tan   +3 more
doaj   +2 more sources

Development of sensitizer peptide-fused endolysin Lys1S-L9P acting against multidrug-resistant gram-negative bacteria [PDF]

Frontiers in Microbiology, 2023
The advent of multidrug-resistant (MDR) bacteria poses a major threat to public health, garnering attention to novel antibiotic replacements. Endolysin, a bacteriophage-derived cell wall-degrading enzyme, is a promising alternative to conventional ...
Su Min Son, Su Min Son, Su Min Son, Joonbeom Kim, Joonbeom Kim, Sangryeol Ryu, Sangryeol Ryu, Sangryeol Ryu   +7 more
doaj   +2 more sources

Potential Role of the Host-Derived Cell-Wall Binding Domain of Endolysin CD16/50L as a Molecular Anchor in Preservation of Uninfected Clostridioides difficile for New Rounds of Phage Infection

Microbiology Spectrum, 2022
Endolysin is a phage-encoded cell-wall hydrolase which degrades the peptidoglycan layer of the bacterial cell wall. The enzyme is often expressed at the late stage of the phage lytic cycle and is required for progeny escape.
Wichuda Phothichaisri, Surang Chankhamhaengdecha, Tavan Janvilisri, Jirayu Nuadthaisong, Tanaporn Phetruen, Robert P. Fagan, Sittinan Chanarat   +6 more
doaj   +1 more source

On the Occurrence and Multimerization of Two-Polypeptide Phage Endolysins Encoded in Single Genes

Microbiology Spectrum, 2022
Bacteriophages (phages) and other viruses are extremely efficient in packing their genetic information, with several described cases of overlapping genes encoded in different open reading frames (ORFs).
Daniela Pinto, Raquel Gonçalo, Mariana Louro, Marta Sousa Silva, Guillem Hernandez, Tiago N. Cordeiro, Carlos Cordeiro, Carlos São-José   +7 more
doaj   +1 more source

Characterization of Salmonella endolysin XFII produced by recombinant Escherichia coli and its application combined with chitosan in lysing Gram-negative bacteria

Microbial Cell Factories, 2022
Background   Salmonella is a common foodborne pathogen, which can cause intestinal diseases. In the last decades, the overuse of antibiotics has led to a pandemic of drug-resistant bacterial infections.
Shuhang Zhang, Yan Chang, Qing Zhang, Yingbo Yuan, Qingsheng Qi, Xuemei Lu   +5 more
doaj   +1 more source

Synthetic antimicrobial peptides as enhancers of the bacteriolytic action of staphylococcal phage endolysins

Scientific Reports, 2022
Bacteriophage endolysins degrade the bacterial cell wall and are therefore considered promising antimicrobial alternatives to fight pathogens resistant to conventional antibiotics. Gram-positive bacteria are usually considered easy targets to exogenously
Ana Gouveia, Daniela Pinto, Helena Veiga, Wilson Antunes, Mariana G. Pinho, Carlos São-José   +5 more
doaj   +1 more source