Results 1 to 10 of about 1,215 (95)

Investigation of Recombinantly Produced Endolysins Reveals a Modular Enzyme Shared by Several Enterobacteria Phages to Exhibit Broad-Range Lytic Activity Against Different Orders of Gammaproteobacteria. [PDF]

Microbiologyopen
The recombinant endolysin El1 derived from Enterobacteria bacteriophages showed potent bactericidal activity against Gram‐negative bacteria when applied exogenously, even in the absence of membrane‐permeabilizing agents, such as ethylenediaminetetraacetic acid (EDTA).
Kazaka T, Zrelovs N, Akopjana I, Bogans J, Jansons J, Dislers A, Kazaks A.   +6 more
europepmc   +2 more sources

Modulation of Endolysin LysECD7 Bactericidal Activity by Different Peptide Tag Fusion

Biomolecules, 2020
The use of recombinant endolysins is a promising approach for antimicrobial therapy capable of counteracting the spread of antibiotic-resistant strains.
Nataliia P. Antonova, Daria V. Vasina, Evgeny O. Rubalsky, Mikhail V. Fursov, Alina S. Savinova, Igor V. Grigoriev, Evgeny V. Usachev, Natalia V. Shevlyagina, Vladimir G. Zhukhovitsky, Vadim U. Balabanyan, Vasiliy D. Potapov, Andrey V. Aleshkin, Valentine V. Makarov, Sergey M. Yudin, Alexander L. Gintsburg, Artem P. Tkachuk, Vladimir A. Gushchin   +16 more
doaj   +3 more sources

Antibacterial Activity of a Fused Endolysin ENDO-1252/KL9P Against Multiple Serovars of Salmonella enterica. [PDF]

Microb Biotechnol
Schematic overview of the construction of fusion endolysin ENDO‐1252/KL9P. (A) The gene for endolysin from the Salmonella phage‐1252 gene (GenBank ID: PP695294.1) was amplified by PCR from the genomic DNA, along with primers containing the endolysin‐linker residues. (B) The amplified endolysin‐linker gene was further re‐amplified using a forward primer
Tung CW, Thapa K, Phan A, Mohapatra A, Hashmi M, Bleich K, Biswas D.   +6 more
europepmc   +2 more sources

Molecular Cloning and Functional Analysis of Holin and Endolysin From Escherichia Phage UE-M6 as Potential Antibacterial Agents. [PDF]

Microbiologyopen
We isolated phage UE‐M6 from wastewater and subsequently cloned, expressed, and purified its encoded endolysin (UE‐lysin) and holin (UE‐holin). Our results demonstrate that the combined application of UE‐lysin and UE‐holin exhibits significantly greater antibacterial activity than either protein alone, indicating a synergistic interaction between the ...
Niaz H, Skurnik M, Adnan F.
europepmc   +2 more sources

Formation of therapeutic phage cocktail and endolysin to highly multi-drug resistant Acinetobacter baumannii: in vitro and in vivo study [PDF]

Iranian Journal of Basic Medical Sciences, 2018
Objective(s): Phage therapy is a potential alternative treatment for infections caused by Acinetobacter baumannii, a significant nosocomial pathogen, which has evolved resistance to almost all conventional antimicrobial drugs in poor hygiene and ...
Hayder Nsaif Jasim, Rand Riadh Hafidh, Ahmed Sahib Abdulamir   +2 more
doaj   +1 more source

Phage Endolysins: Advances in the World of Food Safety

Cells, 2023
As antimicrobial resistance continues to escalate, the exploration of alternative approaches to safeguard food safety becomes more crucial than ever. Phage endolysins are enzymes derived from phages that possess the ability to break down bacterial cell ...
Amina Nazir, Xiaohui Xu, Yuqing Liu, Yibao Chen   +3 more
doaj   +1 more source

2.PHAGE COCKTAILS AGAINST HIGHLY MULTI-DRUG RESISTANT ACINETOBACTER BAUMANII

The Iraqi Journal of Medical Sciences, 2019
Background:Phage therapy is a potential alternative treatment for infections caused by many bacterial species such as Acinetobacter baumannii (A.
Hayder N. Jasim, Ahmad S. Abdul-Ameer
doaj   +6 more sources

PHAGE COCKTAILS AGAINST HIGHLY MULTI-DRUG RESISTANT ACINETOBACTER BAUMANII

The Iraqi Journal of Medical Sciences, 2018
Background:Phage therapy is a potential alternative treatment for infections caused by many bacterial species such as Acinetobacter baumannii (A.
Hayder Jasim, Ahmad Abdul-Ameer
doaj   +1 more source

Lysis-deficient phages as novel therapeutic agents for controlling bacterial infection

BMC Microbiology, 2011
Background Interest in phage therapy has grown over the past decade due to the rapid emergence of antibiotic resistance in bacterial pathogens. However, the use of bacteriophages for therapeutic purposes has raised concerns over the potential for immune ...
Kempashanaiah Nanjundappa, Hariharan Sukumar, Rajagopalan Sanjeev, Sundarrajan Sudarson, Paul Vivek, Padmanabhan Sriram, Sriram Bharathi, Ramachandran Janakiraman   +7 more
doaj   +1 more source

Endolysin Expression, Purification and Activity Determination by Zymography

Bio-Protocol, 2014
Endolysins are peptidoglycan-degrading (muralytic) enzymes produced by many bacteriophages for cell lysis of the host bacterium. The enzymatic activity of muralytic enzymes can be assayed qualitatively using a zymogram containing incorporated ...
Alexander Westbye, Paul Fogg, John Beatty   +2 more
doaj   +1 more source