Investigation of Recombinantly Produced Endolysins Reveals a Modular Enzyme Shared by Several Enterobacteria Phages to Exhibit Broad-Range Lytic Activity Against Different Orders of Gammaproteobacteria. [PDF]
MicrobiologyopenThe recombinant endolysin El1 derived from Enterobacteria bacteriophages showed potent bactericidal activity against Gram‐negative bacteria when applied exogenously, even in the absence of membrane‐permeabilizing agents, such as ethylenediaminetetraacetic acid (EDTA).
Kazaka T +6 more
europepmc +2 more sources
Antibacterial Activity of a Fused Endolysin ENDO-1252/KL9P Against Multiple Serovars of Salmonella enterica. [PDF]
Microb BiotechnolSchematic overview of the construction of fusion endolysin ENDO‐1252/KL9P. (A) The gene for endolysin from the Salmonella phage‐1252 gene (GenBank ID: PP695294.1) was amplified by PCR from the genomic DNA, along with primers containing the endolysin‐linker residues. (B) The amplified endolysin‐linker gene was further re‐amplified using a forward primer
Tung CW +6 more
europepmc +2 more sources
Molecular Cloning and Functional Analysis of Holin and Endolysin From Escherichia Phage UE-M6 as Potential Antibacterial Agents. [PDF]
MicrobiologyopenWe isolated phage UE‐M6 from wastewater and subsequently cloned, expressed, and purified its encoded endolysin (UE‐lysin) and holin (UE‐holin). Our results demonstrate that the combined application of UE‐lysin and UE‐holin exhibits significantly greater antibacterial activity than either protein alone, indicating a synergistic interaction between the ...
Niaz H, Skurnik M, Adnan F.
europepmc +2 more sources
Phage-derived proteins: Advancing food safety through biocontrol and detection of foodborne pathogens. [PDF]
Compr Rev Food Sci Food SafAbstract The emergence of antimicrobial‐resistant foodborne pathogens poses a continuous health risk and economic burden as they can easily spread through contaminated food. Therefore, the demand for new antimicrobial agents to address this problem is steadily increasing.
Choi D, Ryu S, Kong M.
europepmc +2 more sources
Comparative Genomics of 9 Novel Paenibacillus Larvae Bacteriophages [PDF]
, 2016 American Foulbrood Disease, caused by the bacterium Paenibacillus larvae, is one of the most destructive diseases of the honeybee, Apis mellifera. Our group recently published the sequences of 9 new phages with the ability to infect and lyse P.
Amy, Penny S. +4 more
core +2 more sources
Endolysin Expression, Purification and Activity Determination by Zymography
Bio-Protocol, 2014 Endolysins are peptidoglycan-degrading (muralytic) enzymes produced by many bacteriophages for cell lysis of the host bacterium. The enzymatic activity of muralytic enzymes can be assayed qualitatively using a zymogram containing incorporated ...
Alexander Westbye +2 more
doaj +1 more source
The preclinical and clinical progress of bacteriophages and their lytic enzymes : the parts are easier than the whole [PDF]
, 2019 The therapeutic potential of phages has been considered since their first identification more than a century ago. The evident concept of using a natural predator to treat bacterial infections has, however, since then been challenged considerably ...
Abdelkader, Karim Abdelkader Soufi +4 more
core +1 more source
A bacteriophage endolysin that eliminates intracellular streptococci
eLife, 2016 PlyC, a bacteriophage-encoded endolysin, lyses Streptococcus pyogenes (Spy) on contact. Here, we demonstrate that PlyC is a potent agent for controlling intracellular Spy that often underlies refractory infections.
Yang Shen +13 more
doaj +1 more source
Enhancing Whole Phage Therapy and Their Derived Antimicrobial Enzymes through Complex Formulation [PDF]
, 2018 The resurgence of research into phage biology and therapy is, in part, due to the increasing need for novel agents to treat multidrug-resistant infections.
Cooper, Callum +2 more
core +1 more source
Saccharomyces cerevisiae secretion of recombinant bacteriophage endolysin LysKB317 inhibits Limosilactobacillus fermentum in corn mash fermentation [PDF]
Biofuel Research JournalThis study investigated the secretion of endolysin LysKB317 integrated into the HO locus of Saccharomyces cerevisiae strain NRRL Y-2034 to enable the yeast to simultaneously perform ethanol fermentation and control bacterial contaminants frequently ...
Shao-Yeh Lu +4 more
doaj +1 more source