Results 111 to 120 of about 33,711 (238)

Distribution of dwell times of a ribosome: effects of infidelity, kinetic proofreading and ribosome crowding

, 2011
Ribosome is a molecular machine that polymerizes a protein where the sequence of the amino acid residues, the monomers of the protein, is dictated by the sequence of codons (triplets of nucleotides) on a messenger RNA (mRNA) that serves as the template ...
Ajeet K Sharma, Basu M Mohanty P K, Debashish Chowdhury, Dixon M, Frank J, Garai A Chowdhury D, Hill T L, Lakatos G, Shaw L B, Spirin A S, Tinoco I   +10 more
core   +1 more source

Restriction Site-Free Insertion of PCR Products Directionally into Vectors

BioTechniques, 2000
A restriction site-free cloning method has been developed for inserting a PCR product into a vector flexibly and precisely at any desired location with high efficiency. The method uses a pair of DNA integration primers with two portions.
G.J. Chen, N. Qiu, C. Karrer, P. Caspers, M.G.P. Page   +4 more
doaj   +1 more source

Single 23S rRNA mutations at the ribosomal peptidyl transferase centre confer resistance to valnemulin and other antibiotics in Mycobacterium smegmatis by perturbation of the drug binding pocket [PDF]

, 2009
Katherine S. Long, Jacob Poehlsgaard, Lykke H. Hansen, Sven N. Hobbie, Erik C. Böttger, Birte Vester   +5 more
openalex   +1 more source

Structural and mutational analysis of the ribosome-arresting human XBP1u

eLife, 2019
XBP1u, a central component of the unfolded protein response (UPR), is a mammalian protein containing a functionally critical translational arrest peptide (AP). Here, we present a 3 Å cryo-EM structure of the stalled human XBP1u AP. It forms a unique turn
Vivekanandan Shanmuganathan, Nina Schiller, Anastasia Magoulopoulou, Jingdong Cheng, Katharina Braunger, Florian Cymer, Otto Berninghausen, Birgitta Beatrix, Kenji Kohno, Gunnar von Heijne, Roland Beckmann   +10 more
doaj   +1 more source

The DEAD-box protein Dbp6 is an ATPase and RNA annealase interacting with the peptidyl transferase center (PTC) of the ribosome. [PDF]

Nucleic Acids Res, 2023
Khreiss A, Capeyrou R, Lebaron S, Albert B, Bohnsack KE, Bohnsack MT, Henry Y, Henras AK, Humbert O.   +8 more
europepmc   +1 more source

Insights into the Chloramphenicol Inhibition Effect on Peptidyl Transferase Activity, Using Two New Analogs of the Drug [PDF]

, 2011
Ourania N. Kostopoulou
openalex   +1 more source

Mutations in ribosomal protein L3 and 23S ribosomal RNA at the peptidyl transferase centre are associated with reduced susceptibility to tiamulin in Brachyspira spp. isolates [PDF]

, 2004
Märit Pringle, Jacob Poehlsgaard, Birte Vester, Katherine S. Long   +3 more
openalex   +1 more source

Cryoelectron Microscopic Structures of Eukaryotic Translation Termination Complexes Containing eRF1-eRF3 or eRF1-ABCE1

Cell Reports, 2014
Termination and ribosome recycling are essential processes in translation. In eukaryotes, a stop codon in the ribosomal A site is decoded by a ternary complex consisting of release factors eRF1 and guanosine triphosphate (GTP)-bound eRF3.
Anne Preis, Andre Heuer, Clara Barrio-Garcia, Andreas Hauser, Daniel E. Eyler, Otto Berninghausen, Rachel Green, Thomas Becker, Roland Beckmann   +8 more
doaj   +1 more source

Structural basis for the context-specific action of the classic peptidyl transferase inhibitor chloramphenicol. [PDF]

Nat Struct Mol Biol, 2022
Syroegin EA, Flemmich L, Klepacki D, Vazquez-Laslop N, Micura R, Polikanov YS.   +5 more
europepmc   +1 more source

Die Funktion des Responseregulators ARR2 in der Entwicklung von Arabidopsis thaliana [PDF]

, 2004
In dieser Arbeit konnte mittels physiologischer Experimente, Expressionsanalysen, Phosphorylierungsassays und Transaktivierungsanalysen eine komplexe Funktion von ARR2 in verschiedenen Signaltransduktionswegen von Arabidopsis aufgedeckt werden.
Haß, Claudia
core