Alterations at the peptidyl transferase centre of the ribosome induced by the synergistic action of the streptogramins dalfopristin and quinupristin [PDF]
BMC Biology, 2004 Background The bacterial ribosome is a primary target of several classes of antibiotics. Investigation of the structure of the ribosomal subunits in complex with different antibiotics can reveal the mode of inhibition of ribosomal protein synthesis ...
Fucini Paola +4 more
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The Peptidyl Transferase Center: a Window to the Past. [PDF]
Microbiol Mol Biol Rev, 2021 In his 2001 article, “Translation: in retrospect and prospect,” the late Carl Woese made a prescient observation that there was a need for the then-current view of translation to be “reformulated to become an all-embracing perspective about which 21st century Biology can develop” (RNA 7:1055–1067, 2001, https://doi.org/10.1017 ...
Tirumalai MR, Rivas M, Tran Q, Fox GE.
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The Bacillus subtilis ywbD gene encodes RlmQ, the 23S rRNA methyltransferase forming m7G2574 in the A-site of the peptidyl transferase center. [PDF]
RNA, 2023 Wolff P +8 more
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Substrate Specificity of Escherichia coli Peptidyl‐Transferase [PDF]
European Journal of Biochemistry, 1970 The kinetics of the puromycin interaction with different ribosomal bound peptidyl‐tRNAs were studied. The rates of the puromycin reaction with AA‐Phe‐tRNA and AA2‐Phe‐tRNA using three different amino acids (AA) were compared. At 4° all the three dipeptidyl‐tRNAsPhe reacted considerably slower than the three tripeptidyl‐tRNAsPhe. Acetyl‐Phe‐tRNA reacted
Amos Panet, Nathan de Groot, Y. Lapidot
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Hydrolysis of fMet-tRNA by Peptidyl Transferase [PDF]
Proceedings of the National Academy of Sciences, 1971 Escherichia coli and rabbit reticulocyte (f[ 3 H]Met-tRNA·AUG·ribosome) intermediates undergo hydrolysis, with release of f[ 3 H]methionine, upon addition of tRNA or CpCpA in the presence of acetone.
C. Thomas Caskey +3 more
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Structural insights into context-specific inhibition of bacterial translation by macrolides [PDF]
Nature CommunicationsThe ribosome’s peptidyl transferase center (PTC) catalyzes peptide bond formation during protein synthesis and is targeted by many antibiotic classes. Remarkably, macrolides that bind in the peptide exit tunnel some ~10 Å away from the PTC also remotely ...
Egor A. Syroegin +5 more
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Peptidyl transferase center activity observed in single ribosomes
Journal of Molecular Biology, 1999 We demonstrate the functional activity of single ribosomal complexes, opening the way for detailed studies of the trajectories of protein synthesis. Our approach employs a single-molecule detection system, capable of picoseconds to minutes resolution, to observe a growing peptide labeled at its N terminus with the fluorophore tetramethylrhodamine (TMR).
Alexander Sytnik +5 more
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Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome. [PDF]
Nat Struct Mol Biol, 2009 Protein synthesis is catalyzed in the peptidyl transferase center (PTC), located in the large (50S) subunit of the ribosome. No high-resolution structure of the intact ribosome has contained a complete active site including both A- and P-site tRNAs.
Voorhees RM +4 more
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Loss of Conserved rRNA Modifications in the Peptidyl Transferase Center Leads to Diminished Protein Synthesis and Cell Growth in Budding Yeast. [PDF]
Int J Mol SciLeppik M +5 more
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Antibiotic resistance ABCF proteins reset the peptidyl transferase centre of the ribosome to counter translational arrest. [PDF]
Nucleic Acids Res, 2018 Murina V +3 more
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