Piceatannol Alleviates Clostridium perfringens Virulence by Inhibiting Perfringolysin O [PDF]
Molecules, 2022 Clostridium perfringens (C. perfringens) is an important foodborne pathogen that can cause diseases such as gas gangrene and necrotizing enteritis in a variety of economic animals, seriously affecting public health and the economic benefits and healthy ...
Guizhen Wang +8 more
doaj +4 more sources
Single-molecule analysis of the entire perfringolysin O pore formation pathway [PDF]
eLife, 2022 The cholesterol-dependent cytolysin perfringolysin O (PFO) is secreted by Clostridium perfringens as a bacterial virulence factor able to form giant ring-shaped pores that perforate and ultimately lyse mammalian cell membranes. To resolve the kinetics of
Conall McGuinness +7 more
doaj +6 more sources
Perfringolysin O: The Underrated Clostridium perfringens Toxin? [PDF]
Toxins, 2015 The anaerobic bacterium Clostridium perfringens expresses multiple toxins that promote disease development in both humans and animals. One such toxin is perfringolysin O (PFO, classically referred to as θ toxin), a pore-forming cholesterol-dependent ...
Stefanie Verherstraeten +10 more
doaj +6 more sources
Amentoflavone Attenuates Clostridium perfringens Gas Gangrene by Targeting Alpha-Toxin and Perfringolysin O [PDF]
Frontiers in Pharmacology, 2020 Clostridium perfringens (C. perfringens) type A strains are the main cause of gas gangrene in humans and animals. Treatment of this lethal disease is limited, and the prognosis is not good.
Shui Liu +14 more
doaj +5 more sources
Perfringolysin O-Induced Plasma Membrane Pores Trigger Actomyosin Remodeling and Endoplasmic Reticulum Redistribution [PDF]
Toxins, 2019 Clostridium perfringens produces an arsenal of toxins that act together to cause severe infections in humans and livestock animals. Perfringolysin O (PFO) is a cholesterol-dependent pore-forming toxin encoded in the chromosome of virtually all C ...
Cláudia Brito +5 more
doaj +6 more sources
An anti-perfringolysin O monoclonal antibody cross-reactive with streptolysin O protects against streptococcal toxic shock syndrome [PDF]
BMC Research Notes, 2020 Objective Streptococcus pyogenes (Group A Streptococcus; GAS) causes a variety of infections that include life-threatening, severe invasive GAS infections, such as streptococcal toxic shock syndrome (STSS), with > 30% mortality rate, despite effective ...
Takayuki Matsumura +7 more
doaj +5 more sources
Domain 4 (D4) of Perfringolysin O to Visualize Cholesterol in Cellular Membranes—The Update [PDF]
Sensors, 2017 The cellular membrane of eukaryotes consists of phospholipids, sphingolipids, cholesterol and membrane proteins. Among them, cholesterol is crucial for various cellular events (e.g., signaling, viral/bacterial infection, and membrane trafficking) in ...
Masashi Maekawa
doaj +5 more sources
Single-molecule tracking of perfringolysin O assembly and membrane insertion uncoupling. [PDF]
FEBS J, 2023 We exploit single‐molecule tracking and optical single channel recording in droplet interface bilayers to resolve the assembly pathway and pore formation of the archetypical cholesterol‐dependent cytolysin nanopore, Perfringolysin O. We follow the stoichiometry and diffusion of Perfringolysin O complexes during assembly with 60 ms temporal resolution ...
Senior MJT +5 more
europepmc +4 more sources
GST-Perfringolysin O production for the localization and quantification of membrane cholesterol in human and mouse brain and liver [PDF]
STAR Protocols, 2022 Summary: Abnormal cholesterol metabolism is linked to many neurodegenerative disorders. Here, we present a protocol for the production of a recombinant protein consisting of a Glutathione-S-Transferase tag fused with the Perfringolysin O (PFO).
Leire Goicoechea +6 more
doaj +2 more sources
Verbascoside Protects Mice From Clostridial Gas Gangrene by Inhibiting the Activity of Alpha Toxin and Perfringolysin O [PDF]
Frontiers in Microbiology, 2020 Gas gangrene, caused mainly by the anaerobic bacterium Clostridium perfringens (C. perfringens), causes death within 48 h of onset. Limited therapeutic strategies are available, and it is associated with extremely high mortality. Both C.
Jian Zhang +14 more
doaj +2 more sources