Frontiers in Microbiology, 2021 Sepsis caused by Clostridium perfringens infection is rare but often fatal. The most serious complication leading to poor prognosis is massive intravascular hemolysis (MIH).
Ai Suzaki +7 more
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Lethal effects of Clostridium perfringens epsilon toxin are potentiated by alpha and perfringolysin-O toxins in a mouse model [PDF]
Veterinary Microbiology, 2008 Mariano Enrique Fernández-Miyakawa +2 more
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The projection structure of Perfringolysin O (Clostridium perfringens θ‐toxin) [PDF]
FEBS Letters, 1993 The cytolysin Perfringolysin O was applied to lipid layers and the obtained ring‐shaped oligomers analyzed by electron microscopy and image processing. The final result shows the periodic repeat of 2.4 nm along the outer rim of the ring. The asymmetric protein unit, corresponding to one monomer, spans the ring from the convex to the concave surface. It
Olofsson, Anders +2 more
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Microorganisms, 2020 Chlamydia trachomatis and Waddlia chondrophila are strict intracellular bacteria belonging to the Chlamydiales order. C. trachomatis is the most frequent bacterial cause of genital and ocular infections whereas W.
Carole Kebbi-Beghdadi +3 more
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Characterization of Listeria monocytogenes pathogenesis in a strain expressing perfringolysin O in place of listeriolysin O [PDF]
Infection and Immunity, 1994 Listeriolysin O (LLO) is a pore-forming cytolysin that enables Listeria monocytogenes to escape from a host cell vacuole. The structural gene for the related cytolysin perfringolysin O (pfo) was cloned downstream from the promoter for hly, the gene encoding LLO, both on a plasmid and on the L. monocytogenes chromosome. Both strains secreted active PFO,
S, Jones, D A, Portnoy
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Use of an EZ-Tn5-based random mutagenesis system to identify a novel toxin regulatory locus in Clostridium perfringens strain 13. [PDF]
PLoS ONE, 2009 BACKGROUND:Although useful for probing bacterial pathogenesis and physiology, current random mutagenesis systems suffer limitations for studying the toxin-producing bacterium Clostridium perfringens.
Jorge E Vidal +3 more
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PLoS Pathogens, 2008 Reduced tissue perfusion leading to tissue ischemia is a central component of the pathogenesis of myonecrosis caused by Clostridium perfringens. The C.
Michael J Hickey +9 more
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Selective binding of perfringolysin O derivative to cholesterol-rich membrane microdomains (rafts) [PDF]
Proceedings of the National Academy of Sciences, 2001 There is increasing evidence that sphingolipid- and cholesterol-rich microdomains (rafts) exist in the plasma membrane. Specific proteins assemble in these membrane domains and play a role in signal transduction and many other cellular events.
A A, Waheed +8 more
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Decreasing Transmembrane Segment Length Greatly Decreases Perfringolysin O Pore Size [PDF]
The Journal of Membrane Biology, 2015 Perfringolysin O (PFO) is a transmembrane (TM) β-barrel protein that inserts into mammalian cell membranes. Once inserted into membranes, PFO assembles into pore-forming oligomers containing 30-50 PFO monomers. These form a pore of up to 300 Å, far exceeding the size of most other proteinaceous pores.
Qingqing, Lin +3 more
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Single molecule tracking the uncoupling of assembly and membrane insertion in Perfringolysin O [PDF]
, 2021 Abstract We exploit single-molecule tracking and optical single channel recording in droplet interface bilayers to resolve the assembly pathway and pore-formation of the archetypical cholesterol-dependent cytolysin nanopore, Perfringolysin O. We follow the stoichiometry and diffusion of Perfringolysin O complexes during assembly with 60
Michael J T Senior +5 more
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