GST-Perfringolysin O production for the localization and quantification of membrane cholesterol in human and mouse brain and liver [PDF]
STAR Protocols, 2022 Summary: Abnormal cholesterol metabolism is linked to many neurodegenerative disorders. Here, we present a protocol for the production of a recombinant protein consisting of a Glutathione-S-Transferase tag fused with the Perfringolysin O (PFO).
Leire Goicoechea +6 more
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Preparation and utility of asymmetric lipid vesicles for studies of perfringolysin O-lipid interactions. [PDF]
Methods Enzymol, 2021 Studying the interaction of pore-forming toxins, including perfringolysin O (PFO), with lipid is crucial to understanding how they insert into membranes, assemble, and associate with membrane domains. In almost all past studies, symmetric lipid bilayers, i.e., bilayers having the same lipid composition in each monolayer (leaflet), have been used to ...
Kakuda S, Li B, London E.
europepmc +4 more sources
An anti-perfringolysin O monoclonal antibody cross-reactive with streptolysin O protects against streptococcal toxic shock syndrome [PDF]
BMC Research Notes, 2020 Objective Streptococcus pyogenes (Group A Streptococcus; GAS) causes a variety of infections that include life-threatening, severe invasive GAS infections, such as streptococcal toxic shock syndrome (STSS), with > 30% mortality rate, despite effective ...
Takayuki Matsumura +7 more
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Verbascoside Protects Mice From Clostridial Gas Gangrene by Inhibiting the Activity of Alpha Toxin and Perfringolysin O [PDF]
Frontiers in Microbiology, 2020 Gas gangrene, caused mainly by the anaerobic bacterium Clostridium perfringens (C. perfringens), causes death within 48 h of onset. Limited therapeutic strategies are available, and it is associated with extremely high mortality. Both C.
Jian Zhang +14 more
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Inflammasome Activation Induced by Perfringolysin O of Clostridium perfringens and Its Involvement in the Progression of Gas Gangrene [PDF]
Frontiers in Microbiology, 2019 Clostridium perfringens (C. perfringens) is Gram-positive anaerobic, spore-forming rod-shaped bacterial pathogen that is widely distributed in nature. This bacterium is known as the causative agent of a foodborne illness and of gas gangrene.
Kiyonobu Yamamura +8 more
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The Barrier Disruption and Pyroptosis of Intestinal Epithelial Cells Caused by Perfringolysin O (PFO) from Clostridium perfringens [PDF]
CellsClostridium perfringens (C. perfringens), a Gram-positive bacterium, produces a variety of toxins and extracellular enzymes that can lead to disease in both humans and animals.
Zhankui Liu +10 more
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Perfringolysin O as a useful tool to study human sperm physiology
Fertility and Sterility, 2013 To evaluate perfringolysin O, a cholesterol-dependent pore-forming cytolysin, as a tool to study several aspects of human sperm physiology.Prospective study.Basic research laboratory.Human semen samples with normal parameters obtained from healthy donors.Interaction of recombinant perfringolysin O with human spermatozoa.Assessment of perfringolysin O ...
Luis S Mayorga +2 more
exaly +4 more sources
Perfringolysin O structure and mechanism of pore formation as a paradigm for cholesterol-dependent cytolysins. [PDF]
Subcell Biochem, 2014 Cholesterol-dependent cytolysins (CDCs) constitute a family of pore forming toxins secreted by Gram-positive bacteria. These toxins form transmembrane pores by inserting a large β-barrel into cholesterol-containing membrane bilayers. Binding of water-soluble CDCs to the membrane triggers the formation of oligomers containing 35-50 monomers.
Johnson BB, Heuck AP.
europepmc +4 more sources
Structures of perfringolysin O suggest a pathway for activation of cholesterol-dependent cytolysins. [PDF]
J Mol Biol, 2007 Cholesterol-dependent cytolysins (CDCs), a large family of bacterial toxins, are secreted as water-soluble monomers and yet are capable of generating oligomeric pores in membranes. Previous work has demonstrated that large scale structural rearrangements occur during this transition but the detailed mechanism by which these changes take place remains a
Rossjohn J +5 more
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Cholesterol exposure at the membrane surface is necessary and sufficient to trigger perfringolysin O binding. [PDF]
Biochemistry, 2009 Perfringolysin O (PFO) is the prototype for the cholesterol-dependent cytolysins, a family of bacterial pore-forming toxins that act on eukaryotic membranes. The pore-forming mechanism of PFO exhibits an absolute requirement for membrane cholesterol, but the complex interplay between the structural arrangement of the PFO C-terminal domain and the ...
Flanagan JJ +3 more
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