Mechanistic Insights into the Cholesterol-dependent Binding of Perfringolysin O-based Probes and Cell Membranes. [PDF]
Sci Rep, 2017 AbstractCholesterol distribution in the cell is maintained by both vesicular and non-vesicular sterol transport. Non-vesicular transport is mediated by the interaction of membrane-embedded cholesterol and water-soluble proteins. Small changes to the lipid composition of the membrane that do not change the total cholesterol content, can significantly ...
Johnson BB +3 more
europepmc +5 more sources
Functional Dissection of Leishmania major Membrane Components in Resistance to Cholesterol-Dependent Cytolysins [PDF]
ToxinsBacteria use cholesterol-dependent cytolysins (CDCs) to damage eukaryotes. While well-studied in mammals, the mechanisms by which CDCs bind to and kill protozoans remain unclear.
Chaitanya S. Haram +5 more
doaj +2 more sources
The influence of natural lipid asymmetry upon the conformation of a membrane-inserted protein (perfringolysin O). [PDF]
J Biol Chem, 2014 Eukaryotic membrane proteins generally reside in membrane bilayers that have lipid asymmetry. However, in vitro studies of the impact of lipids upon membrane proteins are generally carried out in model membrane vesicles that lack lipid asymmetry. Our recently developed method to prepare lipid vesicles with asymmetry similar to that in plasma membranes ...
Lin Q, London E.
europepmc +4 more sources
The solution structure and oligomerization behavior of two bacterial toxins: pneumolysin and perfringolysin O. [PDF]
Biophys J, 2004 Pneumolysin (PLY), an important protein virulence factor of the human bacterial pathogen Streptococcus pneumoniae, could be a candidate for inclusion in a new anti-streptococcal vaccine. PLY solution species from monomer via multimeric intermediates to ring-shaped oligomers were studied with time-dependent sedimentation velocity in the analytical ...
Solovyova AS +3 more
europepmc +4 more sources
R468A mutation in perfringolysin O destabilizes toxin structure and induces membrane fusion
Biochimica Et Biophysica Acta - Biomembranes, 2017 Perfringolysin O (PFO) belongs to the family of cholesterol-dependent cytolysins. Upon binding to a cholesterol-containing membrane, PFO undergoes a series of structural changes that result in the formation of a β-barrel pore and cell lysis. Recognition and binding to cholesterol are mediated by the D4 domain, one of four domains of PFO.
Michał Dadlez +2 more
exaly +3 more sources
Perfringolysin O expression in Clostridium perfringens is independent of the upstream pfoR gene. [PDF]
J Bacteriol, 2002 ABSTRACT The pathogenesis of Clostridium perfringens -mediated gas gangrene or clostridial myonecrosis involves the extracellular toxins alpha-toxin and perfringolysin O. Previous studies (T. Shimizu, A. Okabe, J. Minami, and H. Hayashi, Infect. Immun.
Awad MM, Rood JI.
europepmc +4 more sources
Hexameric-Based Hierarchy in the Sizes of a Cytolysin Pore-Forming Complex [PDF]
BiomoleculesPerfringolysin O (PFO) is a prototypical member of a large family of pore-forming toxins (PFTs) that are potent virulence factors for many pathogenic bacteria.
Meijun Liu +7 more
doaj +2 more sources
An autopsy case of gas gangrene, massive intravascular hemolysis, and cytokine storm due to Clostridium perfringens type A infection [PDF]
IDCasesClostridium perfringens bacteremia is a rare but rapidly fatal condition, especially in patients exhibiting massive intravascular hemolysis (MIH), gas gangrene, and septic shock. Herein, we present an autopsy case of C.
Akane Hara +8 more
doaj +2 more sources
Journal of Lipid Research, 2023 Almost all the cholesterol in cellular membranes is associated with phospholipids in simple stoichiometric complexes. This limits the binding of sterol ligands such as filipin and perfringolysin O (PFO) to a small fraction of the total. We offer a simple
Yvonne Lange +2 more
doaj +1 more source
Imaging the Assembly of Perfringolysin O [PDF]
Biophysical Journal, 2015 Cholesterol-dependent cytolysins such as Perfringolysin O (PFO) lyse cells by forming large pores in the target cell membrane; they contribute to infections ranging from food poisoning to pneumonia and listeriosis. PFO monomers bind the target membrane and then oligomerize via a pre-pore intermediate to form pores of 20-50 subunits.
Senior, Michael J. +3 more
openaire +1 more source