Domain 4 (D4) of Perfringolysin O to Visualize Cholesterol in Cellular Membranes—The Update [PDF]
Sensors, 2017 The cellular membrane of eukaryotes consists of phospholipids, sphingolipids, cholesterol and membrane proteins. Among them, cholesterol is crucial for various cellular events (e.g., signaling, viral/bacterial infection, and membrane trafficking) in ...
Masashi Maekawa
exaly +7 more sources
Perfringolysin O Theta Toxin as a Tool to Monitor the Distribution and Inhomogeneity of Cholesterol in Cellular Membranes [PDF]
Toxins, 2016 Cholesterol is an essential structural component of cellular membranes in eukaryotes. Cholesterol in the exofacial leaflet of the plasma membrane is thought to form membrane nanodomains with sphingolipids and specific proteins.
Gregory D Fairn +2 more
exaly +8 more sources
Piceatannol Alleviates Clostridium perfringens Virulence by Inhibiting Perfringolysin O [PDF]
Molecules, 2022 Clostridium perfringens (C. perfringens) is an important foodborne pathogen that can cause diseases such as gas gangrene and necrotizing enteritis in a variety of economic animals, seriously affecting public health and the economic benefits and healthy ...
Guizhen Wang +8 more
doaj +4 more sources
Single-molecule analysis of the entire perfringolysin O pore formation pathway [PDF]
eLife, 2022 The cholesterol-dependent cytolysin perfringolysin O (PFO) is secreted by Clostridium perfringens as a bacterial virulence factor able to form giant ring-shaped pores that perforate and ultimately lyse mammalian cell membranes. To resolve the kinetics of
Conall McGuinness +7 more
doaj +6 more sources
Perfringolysin O: The Underrated Clostridium perfringens Toxin? [PDF]
Toxins, 2015 The anaerobic bacterium Clostridium perfringens expresses multiple toxins that promote disease development in both humans and animals. One such toxin is perfringolysin O (PFO, classically referred to as θ toxin), a pore-forming cholesterol-dependent ...
Stefanie Verherstraeten +10 more
doaj +6 more sources
Interaction of Cholesterol with Perfringolysin O: What Have We Learned from Functional Analysis? [PDF]
Toxins, 2017 Cholesterol-dependent cytolysins (CDCs) constitute a family of pore-forming toxins secreted by Gram-positive bacteria. These toxins form transmembrane pores by inserting a large β-barrel into cholesterol-containing membranes.
Alejandro Heuck
exaly +4 more sources
Amentoflavone Attenuates Clostridium perfringens Gas Gangrene by Targeting Alpha-Toxin and Perfringolysin O [PDF]
Frontiers in Pharmacology, 2020 Clostridium perfringens (C. perfringens) type A strains are the main cause of gas gangrene in humans and animals. Treatment of this lethal disease is limited, and the prognosis is not good.
Shui Liu +14 more
doaj +5 more sources
Antibody-Mediated Neutralization of Perfringolysin O for Intracellular Protein Delivery [PDF]
Molecular Pharmaceutics, 2015 Perfringolysin O (PFO) is a member of the cholesterol-dependent cytolysin (CDC) family of bacterial pore-forming proteins, which are highly efficient in delivering exogenous proteins to the cytoplasm. However, the indiscriminate and potent cytotoxicity of PFO limits its practical use as an intracellular delivery system.
Matthew G Vander Heiden +2 more
exaly +7 more sources
Perfringolysin O-Induced Plasma Membrane Pores Trigger Actomyosin Remodeling and Endoplasmic Reticulum Redistribution [PDF]
Toxins, 2019 Clostridium perfringens produces an arsenal of toxins that act together to cause severe infections in humans and livestock animals. Perfringolysin O (PFO) is a cholesterol-dependent pore-forming toxin encoded in the chromosome of virtually all C ...
James Sellers +2 more
exaly +4 more sources
Single-molecule tracking of perfringolysin O assembly and membrane insertion uncoupling. [PDF]
FEBS J, 2023 We exploit single‐molecule tracking and optical single channel recording in droplet interface bilayers to resolve the assembly pathway and pore formation of the archetypical cholesterol‐dependent cytolysin nanopore, Perfringolysin O. We follow the stoichiometry and diffusion of Perfringolysin O complexes during assembly with 60 ms temporal resolution ...
Senior MJT +5 more
europepmc +4 more sources